<i>Bean dwarf mosaic virus</i>: a model system for the study of viral movement

Levy, Avner; Tzfira, Tzvi

doi:10.1111/j.1364-3703.2010.00619.x

Cited by 17 publications

(10 citation statements)

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“…Their genome consists of two components, one is called DNA-A and the other is DNA-B, both are ~2.7 kb in size (Lazarowitz, 1992). Both DNA-A and DNA-B perform different vital roles like viral DNA replication, encapsidation and transcription by DNA-A, while two movement proteins (BV1 and BC1) for translocation of virus by DNA-B (Levy and Tzfira, 2010). New world viruses related to the central and southern American strains such as Tomato leaf deformation virus is a new world virus.…”

Section: Geminivirusesmentioning

confidence: 99%

Transmission Specificity and Coinfection of Mastrevirus with Begomovirus

Khalid¹,

Ur-Rehman²,

Hameed³

et al. 2017

IJAB

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Coat protein (CP) of geminivirus is not requisite for replication of viral DNA yet it is multifunctional and have crucial role in virus transmission by insect vector, systemic infection and virion development. Although in geminivirus, systemic infection rely both on function of CP and specific host-geminivirus-vector interaction but in bipartite begomoviruses CP is dispensable for systemic infection and progression of symptoms. Nevertheless its role in spreading systemic infection cannot be neglected in monopartite viruses' viz. mastrevirus, begomoviruses and curtoviruses, therefore any alteration in CP gene upshot in a new epidemiological adaptation hence worth to study. So the study was designed to present a better picture to view regarding coinfection within different Geminiviruses belonging to mastrevirus and begomovirus particularly due to vector inspecificity consequently resulted in revolutionary recombination events, which ultimately yield new viruses, have more drastic affects on crops. Moreover, co-evolution of both genera may open new horizons in understanding the complexity of cotton leaf curl disease and then to chalk out strategies to counter this emerging threat in Pakistan.

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Section: Geminivirusesmentioning

confidence: 99%

Transmission Specificity and Coinfection of Mastrevirus with Begomovirus

Khalid¹,

Ur-Rehman²,

Hameed³

et al. 2017

IJAB

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“…For some bipartite begomoviruses, such as BDMV, the move-ment complex is not the virion, as capsid protein (CP) mutants are highly infectious (12,27,39,52).…”

mentioning

confidence: 99%

“…Geminiviruses possess small circular single-stranded DNA (ssDNA) genomes encapsidated within twinned icosahedral virions, and they replicate in the nucleus via double-stranded DNA (dsDNA) forms (13,45). To cross nuclear and plasmodesmal boundaries, the bipartite geminiviruses of the genus Begomovirus, such as Bean dwarf mosaic virus (BDMV) (27), encode two proteins from the DNA-B component (35,45,52). The product of the BV1 gene is a nuclear shuttle protein (NSP), whereas that of the BC1 gene is an MP that mediates cell-to-cell trafficking of the infectious DNA through PD (9,24,27,35,45).…”

mentioning

confidence: 99%

“…The product of the BV1 gene is a nuclear shuttle protein (NSP), whereas that of the BC1 gene is an MP that mediates cell-to-cell trafficking of the infectious DNA through PD (9,24,27,35,45). The NSP and MP bind DNA on the basis of size and form, rather than sequence, and this provides specificity for viral DNA forms as well as maintenance of viral genome size (12,14,27,40,43,45). In the current model for cell-to-cell movement of bipartite begomoviruses, the NSP binds to and mediates export of nascent viral DNA from the nucleus.…”

mentioning

confidence: 99%

“…To cross nuclear and plasmodesmal boundaries, the bipartite geminiviruses of the genus Begomovirus, such as Bean dwarf mosaic virus (BDMV) (27), encode two proteins from the DNA-B component (35,45,52). The product of the BV1 gene is a nuclear shuttle protein (NSP), whereas that of the BC1 gene is an MP that mediates cell-to-cell trafficking of the infectious DNA through PD (9,24,27,35,45). The NSP and MP bind DNA on the basis of size and form, rather than sequence, and this provides specificity for viral DNA forms as well as maintenance of viral genome size (12,14,27,40,43,45).…”

mentioning

confidence: 99%

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Histone H3 Interacts and Colocalizes with the Nuclear Shuttle Protein and the Movement Protein of a Geminivirus

Zhou

Rojas

Park

et al. 2011

J Virol

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Geminiviruses are plant-infecting viruses with small circular single-stranded DNA genomes. These viruses utilize nuclear shuttle proteins (NSPs) and movement proteins (MPs) for trafficking of infectious DNA through the nuclear pore complex and plasmodesmata, respectively. Here, a biochemical approach was used to identify host factors interacting with the NSP and MP of the geminivirus Bean dwarf mosaic virus (BDMV). Based on these studies, we identified and characterized a host nucleoprotein, histone H3, which interacts with both the NSP and MP. The specific nature of the interaction of histone H3 with these viral proteins was established by gel overlay and in vitro and in vivo coimmunoprecipitation (co-IP) assays. The NSP and MP interaction domains were mapped to the N-terminal region of histone H3. These experiments also revealed a direct interaction between the BDMV NSP and MP, as well as interactions between histone H3 and the capsid proteins of various geminiviruses. Transient-expression assays revealed the colocalization of histone H3 and NSP in the nucleus and nucleolus and of histone H3 and MP in the cell periphery and plasmodesmata. Finally, using in vivo co-IP assays with a Myc-tagged histone H3, a complex composed of histone H3, NSP, MP, and viral DNA was recovered. Taken together, these findings implicate the host factor histone H3 in the process by which an infectious geminiviral DNA complex forms within the nucleus for export to the cell periphery and cell-to-cell movement through plasmodesmata.Viruses are obligate parasites that utilize host cellular machinery to mediate their replication and infection processes. Plant-infecting viruses encode movement proteins (MPs) that facilitate the cell-to-cell trafficking of their infectious nucleic acids along endogenous pathways, such as plasmodesmata, during the process of systemic infection (11,30,47). Although it is widely recognized that the interactions between virusencoded MPs and host proteins are essential for a viable systemic infection, much remains to be elucidated in terms of the actual pathways involved. Indeed, although a number of host proteins involved in the viral systemic infection process have been identified (47), no consensus pathway has emerged. This likely reflects the diversity of plant viruses and the different cellular pathways utilized.Plant viruses with DNA genomes, such as members of the family Geminiviridae, must utilize cellular components to cross both the nuclear pore and plasmodesmata (PD) for cell-to-cell trafficking of an infectious form of the virus. Geminiviruses possess small circular single-stranded DNA (ssDNA) genomes encapsidated within twinned icosahedral virions, and they replicate in the nucleus via double-stranded DNA (dsDNA) forms (13, 45). To cross nuclear and plasmodesmal boundaries, the bipartite geminiviruses of the genus Begomovirus, such as Bean dwarf mosaic virus (BDMV) (27), encode two proteins from the DNA-B component (35,45,52). The product of the BV1 gene is a nuclear shuttle protein (NSP), whereas t...

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