HyPRP1 performs a role in negatively regulating cotton resistance to V. dahliae via the thickening of cell walls and ROS accumulation

Abstract

BackgroundDeveloping tolerant cultivars by incorporating resistant genes is regarded as a potential strategy for controlling Verticillium wilt that causes severe losses in the yield and fiber quality of cotton.ResultsHere, we identified the gene GbHyPRP1 in Gossypium barbadense, which encodes a protein containing both proline-rich repetitive and Pollen Ole e I domains. GbHyPRP1 is located in the cell wall. The transcription of this gene mainly occurs in cotton roots and stems, and is drastically down-regulated upon infection with Verticillium dahliae. Silencing HyPRP1 dramatically enhanced cotton resistance to V. dahliae. Over-expression of HyPRP1 significantly compromised the resistance of transgenic Arabidopsis plants to V. dahliae. The GbHyPRP1 promoter region contained several putative phytohormone-responsive elements, of which SA was associated with gene down-regulation. We compared the mRNA expression patterns of HyPRP1-silenced plants and the control at the global level by RNA-Seq. A total of 1735 unique genes exhibited significant differential expression. Of these, 79 DEGs involved in cell wall biogenesis and 43 DEGs associated with the production of ROS were identified. Further, we observed a dramatic thickening of interfascicular fibers and vessel walls and an increase in lignin in the HyPRP1-silenced cotton plants compared with the control after inoculation with V. dahliae. Additionally, silencing of HyPRP1 markedly enhanced ROS accumulation in the root tips of cotton inoculated with V. dahliae.ConclusionsTaken together, our results suggest that HyPRP1 performs a role in the negative regulation of cotton resistance to V. dahliae via the thickening of cell walls and ROS accumulation.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1565-1) contains supplementary material, which is available to authorized users.

Genome-Wide Analysis and Expression Profiling of Rice Hybrid Proline-Rich Proteins in Response to Biotic and Abiotic Stresses, and Hormone Treatment

“…In addition, a few other studies have also reported negative effects of HyPRPs on plant’s resistance against biotic stress. In one recent study, using a gain- and loss-of-function approach, Yang et al [40] suggested the role of GbHyPRP1 as a negative regulator of disease resistance in cotton against pathogenic fungi Verticillium dahlia . Using a similar approach, Yoem et al, [39] characterized two HyPRP genes; CaHyPRP1 from C. annuum (pepper) and NbHyPRP1 from Nicotiana benthamiana .…”

“…We also observed the induced expression of OsHyPRP16 under biotic stress and hormone treatments, including ABA, SA, and MeJA (Figure 5D–F). In a recent study, the expression of cotton GbHyPRP was strongly down-regulated in response to SA treatment, while it was significantly up-regulated in each ABA, JA, and ethylene treatment [40]. Based on the similarity in the expression profile of GbHyPRP in response to SA and V. dahlia inoculation, Yang et al [40] proposed a negative role of GbHyPRP towards V. dahlia resistance in cotton via a SA-mediated signaling pathway [40].…”

“…In a recent study, the expression of cotton GbHyPRP was strongly down-regulated in response to SA treatment, while it was significantly up-regulated in each ABA, JA, and ethylene treatment [40]. Based on the similarity in the expression profile of GbHyPRP in response to SA and V. dahlia inoculation, Yang et al [40] proposed a negative role of GbHyPRP towards V. dahlia resistance in cotton via a SA-mediated signaling pathway [40]. Intriguingly, in the present study, the up-regulation of OsHyPRP16 also follows a similar expression pattern in SA-treated and M. oryzae inoculated PB1+ Pi9 (Figure 4 and Figure 5E) [41,42].…”

“…Similarly, tobacco overexpressing JsPRP1 exhibited strong resistance against Colletotrichum gloeosporioides infection [24]. Conversely, the CaHyPRP1 ( Capsicum annuum ) and GbHyPRP1 ( Gossypium barbadense ) were found to negatively regulate the basal defense during biotic stresses, possibly through suppression of ROS [39,40]. All the above studies highlighted the importance of HyPRPs in biotic and abiotic responses, and therefore, it is of great significance to identify and characterize the HyPRPs in plants.…”

Detoxifying processes during kanamycin-induced stress to Arabidopsis thaliana seedling growth

“…Third, oxidative damage after antibiotic-induced oxidative burst stimulates the over-expression of antioxidants. It was reported that ROS balance has been associated with resistance to pathogens in cotton [22,23]. A total of 15 DEGs as key genes of oxidative stress response were involved in antioxidant defense mechanisms (Table 1).…”