Genome-Wide Analysis and Expression Profiling of Rice Hybrid Proline-Rich Proteins in Response to Biotic and Abiotic Stresses, and Hormone Treatment

Abstract

Hybrid proline-rich proteins (HyPRPs) belong to the family of 8-cysteine motif (8CM) containing proteins that play important roles in plant development processes, and tolerance to biotic and abiotic stresses. To gain insight into the rice HyPRPs, we performed a systematic genome-wide analysis and identified 45 OsHyPRP genes encoding 46 OsHyPRP proteins. The phylogenetic relationships of OsHyPRP proteins with monocots (maize, sorghum, and Brachypodium) and a dicot (Arabidopsis) showed clustering of the majority of OsHyPRPs along with those from other monocots, which suggests lineage-specific evolution of monocots HyPRPs. Based on our previous RNA-Seq study, we selected differentially expressed OsHyPRPs genes and used quantitative real-time-PCR (qRT-PCR) to measure their transcriptional responses to biotic (Magnaporthe oryzae) and abiotic (heat, cold, and salt) stresses and hormone treatment (Abscisic acid; ABA, Methyl-Jasmonate; MeJA, and Salicylic acid; SA) in rice blast susceptible Pusa Basmati-1 (PB1) and blast-resistant near-isogenic line PB1+Pi9. The induction of OsHyPRP16 expression in response to the majority of stresses and hormonal treatments was highly correlated with the number of cis-regulatory elements present in its promoter region. In silico docking analysis of OsHyPRP16 showed its interaction with sterols of fungal/protozoan origin. The characterization of the OsHyPRP gene family enables us to recognize the plausible role of OsHyPRP16 in stress tolerance.

SlHyPRP1 and DEA1, the multiple stress responsive eight-cysteine motif family genes of tomato (Solanum lycopersicum L.) are expressed tissue specifically, localize and interact at cytoplasm and plasma membrane in vivo

“…S1). Therefore, the presence of these unique regions confirms that both SlHyPRP1 and DEA1 proteins belong to members of 8CM family proteins (Weyman et al 2006b;Kapoor et al 2019). The phylogenetic analysis of SlHyPRP1 showed 96% similarity between S. lycopersicum and S. pennellii (Fig.…”

“…The new finding from our in vivo interaction assay is the strong interaction between SlHyPRP1 and DEA1. The role of HyPRPs and 8CM family proteins in response to multiple abiotic and biotic stresses have been marked primarily from their expression studies (Kapoor et al 2019). All the HyPRPs reported so far are known to be upregulated by abiotic stresses and down-regulated in response to various biotic stresses (Yang et al 2018).…”

“…HyPRPs present in seed plants belongs to the family of 8CM containing PRPs (Li et al 2016). Apart from plant developmental processes, HyPRPs play important roles in response to different abiotic and biotic stresses (Yang et al 2018;Kapoor et al 2019;Saikia et al 2020). The N-terminal domain of HyPRPs resembles the structural cell wall PRPs but lacks the characteristic amino acid motifs of PRPs (José-Estanyol et al 2004;Dvořáková Lenka 2007).…”

“…Recently, the SlHyPRP1 (S. lycopersicum) and SpHyPRP1 (S. pennellii) genes of tomato were found to be negative regulators of different abiotic stresses such as drought, salinity, cold, oxidative stress, and phytohormone ABA (Li et al 2016;Yang et al 2018). Moreover, SlHyPRP1 has been reported to play a direct or indirect role in defense-related signaling pathways (Kapoor et al 2019).…”

Multivariate Analysis of Amino Acids and Health Beneficial Properties of Cantaloupe Varieties Grown in Six Locations in the United States

“…The amino acids citrulline and proline accumulate in different parts of the plant under stress conditions such as high light intensity or drought, and prevent oxidative injuries. Therefore, these amino acids can be used as biomarkers for selecting drought-tolerant cultivars [ 13 , 14 ]. Amino acids such as alanine, methionine, tryptophan, and phenylalanine are also involved in protein biosynthesis and the formation of secondary metabolites [ 15 ].…”