Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study)

Abstract

Aim:To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro.Materials and Methods:Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p<0.05).Results:The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p<0.05).Conclusion:Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression.

Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells

“…The results showed that 1% hypoxic preconditioning treatment significantly increased CXCR4 and SDF-1 expression, which play an important role in increasing the ability of MSCs to migrate to the damaged area and induce endogenous stem cells to proliferate and differentiate into the desired cells. 9 Thus, it is expected that there will be a process of improving the microenvironment of the resident stem cells, which will eventually lead to the regeneration process. In this study, transplantation of adaptive MSCs was administered by direct injection into salivary glands of Wistar rats damaged by exposure to 15 Gy of single dose ionizing radiations.…”

Advances in Acute Myeloid Leukemia Stem Cells

“…The specific ligand for the CXCR4 chemokine receptor is termed matrix-derived factor-1 (SDF-1, also known as CXCL12). Both CXCR4 and SDF-1 are expressed in various tissues and cell types and regulate cell migration [47]. The SDF-1/CXCR4 axis is also involved in the migration of CSCs [48].…”