Objectives The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O2 concentration.
Materials and Methods Stem cell culture was performed under normoxic (O2: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N2, 5% CO2, and 1% O2. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation.
Statistical Analysis Statistical analysis was done using normality test, followed by MANOVA test (p < 0.05).
Results There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (p < 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia.
Conclusion BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation.
Aim:To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro.Materials and Methods:Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p<0.05).Results:The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p<0.05).Conclusion:Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression.
Objectives: This study is aimed to find out the distribution of DBI in the jaw based on the classification of radiopaque areas and their location using panoramic radiographs at RSKGMP Universitas Airlangga Surabaya.
Materials and Methods: This research was a descriptive observational study with total sampling method. The study used secondary data from panoramic radiographs at the RSKGMP Airlangga University Surabaya during 2018–2021, which had a DBI appearance, and analyzed them based on the classification of radiopaque areas and locations. The results of the analysis are then presented in the form of tables and pie charts.
Results: Classification of DBI based on radiopaque areas was found in type 5 separate (47.81%), type 4 apical (29.82%), type 3 apical and interradicular (17.54%), type 1 interradicular (3.07%), and the least in type 2 interradicular and separate (1.75%). The most common locations of the lesions were in the premolar region (42.54%), the molar region (27.63%), the canine region (12.28%), the canine-premolar region (8.33%), the premolar-molar region (6.58%), and the least common location in the incisor and incisor-canine regions (1,32%).
Conclusion: Classification of DBI based on the most common radiopaque area was found to be type 5 separate, with the most lesion locations in the premolar region. While the least classification was found in type 2 interradicular and separate, the incisive and incisive-canine regions had the fewest lesion locations.
Objectives: Panoramic radiographs are used as a diagnostic tool adjunctive to oral examinations, treatment plans, and evaluation of treatment outcomes. This study was aimed to determine the distribution of Dense Bone Island (DBI) in the jaws through panoramic radiography based on gender and age.
Materials and Methods: The method used for this research is descriptive-observational. The research data used is secondary data with a total of 422 panoramic radiographic data of RSKGMP Airlangga University patients.
Results: From a study of 422 secondary data found 253 (60%) patients with Dense Bone Island in the jaw, more commonly found in female, which is 162 (38.4%) in female patients and 91 (21.5%) in male patients. It was also found mostly in the age range of 17–25 years.
Conclusion: Dense Bone Island was found more in female than in male. It was also found to be more common in patients aged 17–25 years.
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