Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells

Iida, Kei; Takeda-Kawaguchi, Tomoko; Tezuka, Yoshihiko; Kunisada, Takahiro; Shibata, Toshiyuki; Tezuka, Kenichi

doi:10.1016/j.archoralbio.2010.06.005

Cited by 85 publications

(64 citation statements)

References 22 publications

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“…Our results are in agreement with an earlier report wherein hypoxia was shown to amplify the proliferation of DPSC [Sakdee et al, 2009]. The hypoxia condition was expected to be suitable for the establishment and maintenance of human dental pulp stem cells because they reside in a low-oxygen environment in vivo [Iida et al, 2010]. Apart from enhancing cell proliferation, hypoxia has been claimed to enhance the angiogen- ic potential of human DPSC.…”

Section: Discussionsupporting

confidence: 83%

Influence of Hypoxia, High Glucose, and Low Serum on the Growth Kinetics of Mesenchymal Stem Cells from Deciduous and Permanent Teeth

Kanafi

Ramesh

Gupta

et al. 2013

Cells Tissues Organs

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The therapeutic potential of mesenchymal stromal cells depends on their ability to survive and proliferate under adverse in vivo scenarios in a particular disease. In most of the sites of injury, especially in diabetic wounds, there can be hypoxia, hyperglycemia, and ischemia, leading to a lack of nutrients. Hence, the aim of our present study was to investigate the influence of hypoxia, high glucose, and low serum concentrations on the growth kinetics and proliferative potential of human dental pulp stem cells from exfoliated deciduous teeth (SHED) and permanent teeth (DPSC). In this study we isolated two types of specialized stem cells from human dental pulp tissues, which were supposedly of neural crest origin, and cultured them in KO-DMEM medium supplemented with 10% fetal bovine serum (FBS). Both SHED and DPSC were characterized for standard CD surface markers, and their ability to differentiate into adipogenic and osteogenic lineages was tested. SHED and DPSC were exposed to either hypoxia or high glucose or low serum conditions, and their growth kinetics and differentiation potentials were compared with those of normal culture conditions. We found that SHED retained their phenotypic expression and differentiation potential under hypoxia, high-glucose, and low-serum conditions and exhibited a higher proliferation in terms of cell yield and a reduced doubling time compared to DPSC. Our findings clearly demonstrate for the first time that SHED are superior to DPSC as evidenced by their enhanced proliferation under adverse culture conditions.

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Section: Discussionsupporting

confidence: 83%

Influence of Hypoxia, High Glucose, and Low Serum on the Growth Kinetics of Mesenchymal Stem Cells from Deciduous and Permanent Teeth

Kanafi

Ramesh

Gupta

et al. 2013

Cells Tissues Organs

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“…Osteogenic differentiation was confirmed by Alizarin red S staining and RT-PCR for Osterix gene. Densitometry analysis of PCR amplified cDNA of osterix from two groups revealed marginally lower expression of this osteogenic marker gene in induced hypoxic culture [7,31]. Similar observation has been recently reported in case of Adipose Stem Cells, where hypoxia was found to inhibit osteogenic differentiation [32,33].…”

Section: Effect Of Hypoxia On Stemness and Differentiation Of Dental supporting

confidence: 67%

Effect of Hypoxia on Stemness and Differentiation of Dental Pulp Derived Stem Cells

Kakkar¹,

Sharma²,

Sankar³

et al. 2016

IOSR

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“…30 Thus, older patients’ teeth contain fewer DPSC colonies than the teeth of younger patients. 31 There is some conflicting evidence, as some groups have reported no such age-related change, but the majority of studies find an age-related decrease in differentiation/regeneration capacity of cells derived from dental pulp. Human DPSCs obtained from older donors are thought to lose their proliferative activity and differentiation capability, and become senescent, after fewer passages than cells derived from younger donors.…”

Section: Discussionmentioning

confidence: 99%

Retrieval of a periodontally compromised tooth by allogeneic grafting of mesenchymal stem cells from dental pulp: A case report

et al. 2018

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ObjectiveTo report a case of successful allogeneic grafting of mesenchymal dental pulp stem cells (DPSCs) as preliminary findings in a patient with periodontal disease enrolled into clinical trial ISRCTN12831118.MethodsMesenchymal stem cells from the dental pulp of a deciduous tooth from a 7-year-old donor were separated from the pulp chamber and processed via enzymatic digestion and centrifugation. DPSCs were passaged and cultured on a 35 × 13 mm culture dish in minimum essential medium-alpha, without supplementation. After reaching 80% confluency, 5 x 106 allogeneic DPSCs in 250 µl phosphate buffered saline were seeded onto a dry scaffold of lyophilized collagen-polyvinylpyrrolidone sponge placed in the left lower premolar area of a 61-year-old patient with periodontal disease. Surgical access to the lower premolar area was achieved using the flap technique.ResultsAt 3 and 6 months following allogeneic graft, the patient showed no sign of rejection and exhibited decreases in tooth mobility, periodontal pocket depth and bone defect area. Bone mineral density had increased at the graft site.ConclusionsRegenerative periodontal therapy using DPSCs of allogeneic origin may be a promising treatment for periodontal disease-induced bone defects.

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Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells

Cited by 85 publications

References 22 publications

Influence of Hypoxia, High Glucose, and Low Serum on the Growth Kinetics of Mesenchymal Stem Cells from Deciduous and Permanent Teeth

Influence of Hypoxia, High Glucose, and Low Serum on the Growth Kinetics of Mesenchymal Stem Cells from Deciduous and Permanent Teeth

Effect of Hypoxia on Stemness and Differentiation of Dental Pulp Derived Stem Cells

Retrieval of a periodontally compromised tooth by allogeneic grafting of mesenchymal stem cells from dental pulp: A case report

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