Hyphenation of capillary zone electrophoresis with mass spectrometry for proteomic analysis: Optimization and comparison of two coupling interfaces

Gou, Marie-Jia; Nys, Gwenaël; Cobraiville, Gaël; Demelenne, Alice; Servais, Anne-Catherine; Fillet, Marianne

doi:10.1016/j.chroma.2020.460873

Cited by 14 publications

(17 citation statements)

References 35 publications

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“…To enrich weakly acidic and basic analytes, the difference in the pH between the sample matrix and BGE can be employed, which is known as the dynamic pH junction or the pH-mediated stacking. During the year 2019-2021, several reports on the pH-mediated stacking techniques have appeared for the CE analyses of p-cresols [62], peptides [63], kynurenine enantiomers [64], and sulfur-containing amino acids [65] as summarized in Table 5. Zhao et al reported a specific pH-mediated stacking technique with multisegmented injection to avoid ion suppression in CE-MS [66] for the determination of sulfur metabolites [65].…”

Section: Application Of Ph-mediated Stackingmentioning

confidence: 99%

Recent Applications of Dynamic On-Line Sample Preconcentration Techniques in Capillary Electrophoresis

Kitagawa

SOMA

2022

Chromatography

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This review highlights recent applications of on-line sample preconcentration techniques in capillary electrophoresis (CE) from 2019 to 2021. Various preconcentration techniques based on the analyte velocity change in two or three discontinuous solutions system including field-amplified sample stacking, transient isotachophoresis, pH-mediated stacking, sweeping, and their modified and combined techniques have been employed to enrich and separate biological, environmental, food samples, and so on, in CE. More than 60 published research articles collected from Scopus databases described the dynamic on-line sample preconcentration techniques. This review provides comprehensive tables listing the applications of the on-line sample preconcentration techniques.

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Section: Application Of Ph-mediated Stackingmentioning

confidence: 99%

Recent Applications of Dynamic On-Line Sample Preconcentration Techniques in Capillary Electrophoresis

Kitagawa

SOMA

2022

Chromatography

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“…However, despite the significant gain in sensitivity, the practical use is still impaired due to technical difficulties in assembling the interface, blocked emitter tips by particles and limited flushing routines of background electrolyte and sheath liquid. Also, manual replenishment of sheath liquid can be tedious and lead to uncontrolled electrolyte and sheath liquid compositions in the emitter tip by accident, as described in a recent publication [16]. These drawbacks prevent routine application of these nanointerfaces.…”

Section: Figurementioning

confidence: 99%

Two capillary approach for a multifunctional nanoflow sheath liquid interface for capillary electrophoresis‐mass spectrometry

et al. 2020

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CE hyphenated to ESI‐MS (CE‐ESI‐MS) is a well‐established technique to analyze charged analytes in complex samples. Although various interfaces for CE‐MS coupling are commercially available, the development of alternatives which combine sensitivity, simplicity, and robustness remains a topic of research. In this work, a nanoflow sheath liquid CE‐MS interface with two movable capillaries inside a glass emitter is described. The setup enables a separation mode and a conditioning mode to guide the separation capillary effluent either into the electrospray or to the waste, respectively. This enables to exclude parts of the analysis from MS detection and unwanted matrix components reaching the mass spectrometer, comparable to divert valves in LC‐MS coupling. Also, this function improves the overall robustness of the system by reduction of particles blocking the emitter. Preconditioning with electrospray interfering substances and even the application of coating materials for every analysis is enabled, even while the separation capillary is built into the interface with running electrospray. The functionality is demonstrated by analyses of heavy matrix bioreactor samples. Overall, this innovation offers a more convenient installation of the interface, improved handling with an extended lifetime of the emitter tips and additional functions compared to previous approaches, while keeping the higher sensitivity of nanoflow CE‐MS‐coupling.

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“…9−11 Other interfaces employ electrospray from the tip of the stainless steel or glass emitter. 7,12−14 The value of nanoflow SL interfaces to increase sensitivity in CE-MS has been proven several times recently, 7,10,15,16 and the commercialization of the interface developed by the Dovichi group as the EMASS-II interface (CMP Scientific) enabled access to this technique to a broader scientific community. 13 However, the setup and operation of these interfaces are laborious and need some experience and expertise.…”

Section: ■ Introductionmentioning

confidence: 99%

“…13 However, the setup and operation of these interfaces are laborious and need some experience and expertise. 16,17 Additionally, problems with bubble formation and emitter clogging hamper the analytical ruggedness.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The value of nanoflow SL interfaces to increase sensitivity in CE-MS has been proven several times recently, ,,, and the commercialization of the interface developed by the Dovichi group as the EMASS-II interface (CMP Scientific) enabled access to this technique to a broader scientific community . However, the setup and operation of these interfaces are laborious and need some experience and expertise. , Additionally, problems with bubble formation and emitter clogging hamper the analytical ruggedness.…”

Section: Introductionmentioning

confidence: 99%

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nanoCEasy: An Easy, Flexible, and Robust Nanoflow Sheath Liquid Capillary Electrophoresis-Mass Spectrometry Interface Based on 3D Printed Parts

et al. 2021

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Capillary electrophoresis-mass spectrometry (CE-MS) is a powerful tool in various fields including proteomics, metabolomics, and biopharmaceutical and environmental analysis. Nanoflow sheath liquid (SL) CE-MS interfaces provide sensitive ionization, required in these fields, but are still limited to a few research laboratories as handling is difficult and expertise is necessary. Here, we introduce nanoCEasy, a novel nanoflow SL interface based on 3D printed parts, including our previously reported two capillary approach. The customized plug-and-play design enables the introduction of capillaries and an emitter without any fittings in less than a minute. The transparency of the polymer enables visual inspection of the liquid flow inside the interface. Robust operation was systematically demonstrated regarding the electrospray voltage, the distance between the emitter and MS orifice, the distance between the separation capillary and emitter tip, and different individual emitters of the same type. For the first time, we evaluated the influence of high electroosmotic flow (EOF) separation conditions on a nanoflow SL interface. A high flow from the separation capillary can be outbalanced by increasing the electrospray voltage, leading to an overall increased electrospray flow, which enables stable operation under high-EOF conditions. Overall, the nanoCEasy interface allows easy, sensitive, and robust coupling of CE-MS. We aspire the use of this sensitive, easy-to-use interface in large-scale studies and by nonexperts.

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Hyphenation of capillary zone electrophoresis with mass spectrometry for proteomic analysis: Optimization and comparison of two coupling interfaces

Cited by 14 publications

References 35 publications

Recent Applications of Dynamic On-Line Sample Preconcentration Techniques in Capillary Electrophoresis

Recent Applications of Dynamic On-Line Sample Preconcentration Techniques in Capillary Electrophoresis

Two capillary approach for a multifunctional nanoflow sheath liquid interface for capillary electrophoresis‐mass spectrometry

nanoCEasy: An Easy, Flexible, and Robust Nanoflow Sheath Liquid Capillary Electrophoresis-Mass Spectrometry Interface Based on 3D Printed Parts

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