Recent Applications of Dynamic On-Line Sample Preconcentration Techniques in Capillary Electrophoresis

Kitagawa, Fumihiko; SOMA, Yuta

doi:10.15583/jpchrom.2022.012

Cited by 8 publications

(5 citation statements)

References 86 publications

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“…In addition to the above chromatographic sorptionbased principles, peptide preseparation and preconcentration can be achieved by online electro-driven samplestacking techniques [87,88]. They include field amplified sample stacking [89], field-enhanced or field-amplified sample injection (FESI or FASI) [77,90,91], large volume sample stacking (LVSS) [92][93][94], transient-ITP (t-ITP) [95,96], electrokinetic supercharging [97,98], and dynamic pH barrage junction and pH-mediated stacking [93,99].…”

Section: Preconcentration and Preseparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Preconcentration and Preseparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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“…On-line preconcentration strategies are simpler, more economical, and can reduce manipulation with samples, lowering the chance of errors compared to offline preconcentration methods. Therefore, on-line sample preconcentration strategies could be considered the most advantageous to overcome the sensitivity limitation of CE [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Sensitivity enhancement of capillary electrophoresis‐frontal analysis‐based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Bržezická,

Glatz,

Kohútová

2023

J of Separation Science

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Capillary electrophoresis-frontal analysis is one of the most frequently used approaches for the study of plasma protein-drug interactions as a substantial part of new drug development. However, the capillary electrophoresis-frontal analysis typically combined with ultraviolet-visible detection suffers from insufficient concentration sensitivity, particularly for substances with limited solubility and low molar absorption coefficient. The sensitivity problem has been solved in this work by its combination with an on-line sample preconcentration. According to the knowledge of the authors this combination has never been used to characterize plasma protein-drug binding. It resulted in a fully automated and versatile methodology for the characterization of binding interactions. Further, the validated method minimalizes the experimental errors due to a reduction in the manipulation of samples. Moreover, employing an on-line preconcentration strategy with capillary electrophoresis-frontal analysis using human serum albumin-salicylic acid as a model system improves the drug concentration sensitivity 17-fold compared to the conventional method. The value of binding constant (1.51 ± 0.63) ⋅ 10 4 L/mol obtained by this new capillary electrophoresisfrontal analysis modification is in agreement with the value (1.13 ± 0.28) ⋅10 4 L/mol estimated by a conventional variant of capillary electrophoresis-frontal analysis without the preconcentration step, as well as with literature data obtained using different techniques.

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“…Although their origins are sufficiently old, the development of novel separation methods based on HPLC and electrophoresis are maintained. Both fundamental and applicational studies are still progressing to analyze a wide variety of compounds [1][2][3][4][5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Development of Novel Analytical Methods Based on Liquid Chromatography and Electrophoresis

Kitagawa

2023

Chromatography

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High-performance liquid chromatography (HPLC) and electrophoresis are liquid phase separation methods and are frequently used in various research fields. Although their origins are sufficiently old, the development of novel separation methods based on HPLC and electrophoresis are still required for both fundamental and applicational functions. In this paper, we introduced our fundamental studies of the development of novel analytical methods based on HPLC and electrophoresis toward enhancing separation efficiency. The contents can be categorized as follows: (1) enhancement of separation in pressured flow-driven capillary electrochromatography, (2) orthogonal electrochromatography for simultaneous two-dimensional separation, (3) development of new stationary phases/columns in HPLC, (4) development of low and ultra-low-temperature HPLC using liquefied gas mobile phase, (5) development of nonaqueous capillary electrophoresis for analyzing water-insoluble synthetic polymers, and (6) development of frequency division multiplex HPLC-MS.

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Recent Applications of Dynamic On-Line Sample Preconcentration Techniques in Capillary Electrophoresis

Cited by 8 publications

References 86 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Sensitivity enhancement of capillary electrophoresis‐frontal analysis‐based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Development of Novel Analytical Methods Based on Liquid Chromatography and Electrophoresis

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