Development of Novel Analytical Methods Based on Liquid Chromatography and Electrophoresis

Kitagawa, Shinya

doi:10.15583/jpchrom.2022.022

Cited by 5 publications

(3 citation statements)

References 72 publications

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“…The important role of multidimensional separations of biopeptides, peptide fragments of proteins and proteins in proteomic, peptidomic, and metabolomic studies was highlighted in the recent reviews [13,39,41]. Two-or multidimensional peptide separations are based on the combination of two or more separation methods with different separation principles, for example, LC and electrophoresis [181,182]. If these principles are independent, the methods are classified as "orthogonal," and the peak capacity of this 2D separation is approximately equal to the product of the individual peak capacities of each dimension [183].…”

Section: Multidimensional Separationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Multidimensional Separationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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“…In macromolecular porous media, there is porous polymers (monoliths) of which frames and sizes can be controlled individually with high strength as monoliths involve steric entanglement of thin frames in 3D networks and comparatively large voids (through-pores) as well as consecutive and cyclic structures. The typical polymer monoliths are fabricated by copolymerizing with hydrophobic cross-linkers and monofunctional monomers in mixed porogens in the presence of initiators [1][2][3][4]. The radicals that are generated by heat or irradiation with UV light lead fabrication of polymers of which specific morphology forms via complex relations between polymerization and phase separation processes.…”

Section: Introductionmentioning

confidence: 99%

Antimold Activities Using Quaternized Epoxy-Based Polymer Monoliths

KUBO,

NAKAMICHI,

K-YAMADA

et al. 2024

Chromatography

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Monolithic materials having 3D-network structure are now widely used for the separation such in liquid chromatography and solid-phase extraction. Additionally, monolithic materials showed several functionalities, we previously discovered that epoxy monoliths, which were prepared with epoxy compounds and amine curing agents using suitable porogens, showed antibacterial potency when quaternized by hydrogen halides and alkyl halides and immersed in coli suspension. Antibacterial activity was enhanced by lowering pH of the aqueous phase with the former and cell membrane disruption triggered by cell contact with the latter. As well as the epoxy monoliths with nitrogen (N) at the high content rate enhance antibacterial activity. We consider that these actions may be effective for molds. In this study, we synthesize epoxy monoliths, conducting quaternization with hydrochloric acid and butyl bromide. Alternaria alternate, one of the genera in sooty mold, is employed for an evaluation. We examine effects of the epoxy monoliths on spores and hyphal growth. With the effects on spores, unquaternized monoliths and ones quaternized with hydrochloric acid and/or butyl bromide were immersed in coli suspension, and after predetermined time, the suspension was cultured in potato dextrose agar. Consequently, the monoliths quaternized with butyl bromide inhibited the growth of the spores. With the effects on the hyphal growth, we observed mold growth by placing the fabricated epoxy monolith in PDA and injecting molds to the surface of the monolith. The monoliths quaternized with hydrochloric acid inhibited the hyphal growth most by lowering pH of the culture medium. The quaternized monoliths with butyl bromide could inhibit the hyphal growth due to the positively charged surface of the monolith. Besides, we successfully achieved antimold with inhibiting the fungal growth by raising the N content rate and increasing degree of quaternization.

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“…Consequently, its use as a stationary phase in LC presents inevitable limitations, including the available temperature range and mobile phase solvents, because of the heat and/or chemical resistance of silicagel particles. Many types of novel stationary phases with retention trends different from those of conventional stationary phases have been developed to achieve an improved separation performance for certain complex samples in LC [20][21][22][23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Fibrous Polyimide Stationary Phase for the Separation of Aromatic Compounds in Reversed-Phase Liquid Chromatography

NAKAGAMI,

YAMAGUCHI,

KONO

et al. 2024

Chromatography

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A novel fiber-packed column was developed with a fibrous polyimide (PI) as the separation medium in liquid chromatography (LC), and the retention behavior for various aromatic compounds on the stationary phase was investigated in reversed-phase conditions. A retention trend was observed on the PI phase, which strongly retained certain types of analytes with long alkyl chain(s) in the chemical structure. However, the retention factor of analytes with short alkyl chains exceeded that expected from hydrophobic interaction between the analytes and stationary phase. Several solute pairs with and without polar functional groups were employed as the sample probe. The separation factor for these analyte pairs on the PI phase was significantly better than that on typical conventional stationary phases such as octadecylsilica phases. The fibrous PI stationary phase exhibited a unique retention tendency in that the retention time of toluene increased with an increase in the column temperature, and the trends differed from that observed on typical LC stationary phases.

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Development of Novel Analytical Methods Based on Liquid Chromatography and Electrophoresis

Cited by 5 publications

References 72 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Antimold Activities Using Quaternized Epoxy-Based Polymer Monoliths

Fibrous Polyimide Stationary Phase for the Separation of Aromatic Compounds in Reversed-Phase Liquid Chromatography

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