Hyperoside protects against cerebral ischemia-reperfusion injury by alleviating oxidative stress, inflammation and apoptosis in rats

Abstract

This study investigated the effects of hyperoside pre-treatment on the oxidative stress, inflammatory response and apoptosis in rats with cerebral ischemia-reperfusion (I/R) injury. SD rats were randomly divided into sham, I/R, hyperoside (I/R þ Hyp) and nimodipine (I/R þ Nim) groups. The rats in the hyperoside group and the nimodipine group were intra-gastrically given hyperoside (50 mg/kg/day) and nimodipine (15 mg/kg/day), respectively, 15 days before operation. A rat cerebral ischemia model was established by suture-occlusion 1 h after the last administration. The thread embolus was removed 2 h after the ischemia for the 24 h reperfusion. The results showed that compared with that in the I/R model group, the neurologic function of the hyperoside group rats was significantly improved and the cerebral infarction volume ratio decreased significantly. The total antioxidant capacity, the activity of superoxide dismutase and glutathione peroxidase in the brain tissue of the hyperoside group rats were significantly higher than those in the I/R model group. The content of malondialdehyde, interleukin-1b and tumour necrosis factor-a in the brain tissue of the hyperoside group rats was significantly lower than that in the I/R model group. Compared with that in the I/R model group, the expression of Bcl-2 mRNA, and p-PI3K and p-AKT proteins in the hyperoside group increased significantly, whereas that of Bax and caspase-3 mRNA decreased significantly. These results suggest that hyperoside had a significant protective effect against cerebral I/R injury in rats, which is related to its inhibiting the oxidative stress, alleviating the inflammatory response and having anti-apoptotic effects.

Phenolic profile, antioxidant and enzyme inhibitory activity of the ethyl acetate, methanol and water extracts of Capparis spinosa L.

“…However, as mentioned above, the presence of hesperidin and hyperoside in this plant was brought to the literature for the first time with this study. There are some literature data on the contribution of these compounds to antioxidant activity with some other plants or with these compounds themselves (Ku et al, 2014;Hao et al, 2016;Yatao et al, 2018;Gao et al, 2019;He et al, 2019;Kim et al, 2019;Musa et al, 2019;Aggarwal et al, 2020;Huang et al, 2020). These findings support the correlation coefficient data obtained from the present study.…”

<p>MicroRNA-579-3p Exerts Neuroprotective Effects Against Ischemic Stroke via Anti-Inflammation and Anti-Apoptosis</p>

“…And the reperfusion injury caused by cerebral I/R, including inflammation, apoptosis and so on, plays a key role in the process of I/R injury. 34 MicroRNA (miRNA) is involved in almost all known biological regulatory processes, such as cell differentiation, apoptosis, etc., and its effect is very potent. 35,36 It can be highly expressed as a biological marker in the human circulating blood system.…”

Changes in Growth and Photosynthetic Parameters and Medicinal Compounds in Eleutherococcus senticosus Harms under Drought Stress

“…This could be explained by the fact that phenylpropanoids and shikimate pathway enzymes reduce their activity under drought stress, as has been demonstrated in other plants (Mewis et al, 2012;Sanchez-Rodriguez et al, 2011). Previous studies (He et al, 2019;Liu et al, 2019;Qian et al, 2019) have shown that these compounds have antiinfective, immunomodulatory, and other pharmacological effects. Our results show that Eleuthero leaves have good medicinal value.…”