Hyperin Extracted from Manchurian Rhododendron Leaf Induces Apoptosis in Human Endometrial Cancer Cells Through a Mitochondrial Pathway

Abstract

Background: A number of effective prevention measures have been introduced in attempts to substantially reduce both the incidence and mortality due to many kinds of cancer. The search for new anti-cancer compounds in foods or in plant medicines is one realistic and promising approach to prevention. Chinese medicines provide a rich pool of novel and efficacious agents for cancer prevention and treatment. Previously it was demonstratrated that hyperin extracted from the Manchurian rhododendron leaf reduces the proliferation of many cancer cells. The present study was carried out to evaluate its effects on human endometrial cancer cell viability and apoptosis and to investigate its mechanisms of action in RL952 cells. Methods: Cell viability was measured using the MTT assay. Intracellular calcium ions were detected using laser-scanning confocal microscopy. The effects of hyperin on apoptosis related proteins in RL952 cells were examined using Western blot analysis. Results: The growth of RL952 cells was inhibited by treatment with hyperin. OD values of caspase-3 and caspase-9 were increased and expression of bcl-2 was increased and bax was decreased in protein levels in RL952 cells after 24 h of hyperin treatment, Moreover, intracellular calcium accumulation occurred in hyperin-treated cells. Conclusions: These results suggest that hyperin may play an important role in tumor growth suppression by inducing apoptosis in human endometrial cells via a Ca 2+ -related mitochondrion apoptotic pathway in RL952 cells.

Hyperoside exerts anti-inflammatory and anti-arthritic effects in LPS-stimulated human fibroblast-like synoviocytes in vitro and in mice with collagen-induced arthritis

“…Hyperoside is a flavonol glycoside mainly found in plants of the genera Hypericum and Crataegus [29] . Pharmacological investigations have demonstrated that hyperoside has anti-oxidant [30] , anti-cancer [31] and anti-inflammatory activities [32][33][34] . This compound exerts anti-inflammatory effects by suppressing the production of proinflammatory cytokines through inhibiting the activation of the NF-κB signaling pathway in human endothelial cells [33] .…”

PaCaHa inhibits proliferation of human cancer cells in vitro

“…The 2008 World Health Organization (WHO) cancer report estimated that worldwide deaths are likely to rise to over 11 million in 2030; therefore, new, safe, and effective anticancer agents are needed (5). In recent years, the anticancer effects of synthetic chemicals and natural extracts of plants have gained prominence (9).…”

“…All these factors have led scientists to continue to develop alternative cancer treatment strategies (5). In this context, a number of synthetic compounds and natural products were evaluated for their efficacy.…”

Hyperoside induces both autophagy and apoptosis in non-small cell lung cancer cells in vitro

“…Quercetin-3-O-β-D-galactopyranoside, also known as hyperoside, is a flavonol glycoside mainly found in plants of the genera Hypericum and Crataegus [20] . Previous studies have shown that hyperoside has anti-oxidant [21] , anticancer [22,23] and anti-inflammatory activities [24][25][26] . Furthermore, hyperoside produced anti-cancer effects through inducing apoptosis [22] and inhibiting of oncogenic microRNA-27a [27] .…”

“…Previous studies have shown that hyperoside has anti-oxidant [21] , anticancer [22,23] and anti-inflammatory activities [24][25][26] . Furthermore, hyperoside produced anti-cancer effects through inducing apoptosis [22] and inhibiting of oncogenic microRNA-27a [27] . However, whether hyperoside induces autophagy, and the role it plays in cell death in human lung cancer cells, remain unclear.…”