Hyperoside induces both autophagy and apoptosis in non-small cell lung cancer cells in vitro

Fu, Ting; Wang, Ling; Jin, Xiangnan; Sui, Hai-juan; Liu, Zhou; Jin, Ying

doi:10.1038/aps.2015.148

Cited by 51 publications

(35 citation statements)

References 55 publications

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“…In another study, hyperoside dose-dependently (0.5, 1, 2 mmol/L) increased the expression of LC3-II and autophagosome numbers in human non-small cell lung cancer cell line A549 cells; however, such effects in human bronchial epithelial cell line BEAS-2B cells were not observed. Moreover, hyperoside inhibited the phosphorylation of Akt, mTOR, p70S6K and 4E-BP1, but increased the phosphorylation of ERK1/2 in A549 cells (Fu et al, 2016).…”

Section: Venskutonismentioning

confidence: 86%

Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): review of recent research advances

Venskutonis¹

2018

JFB

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Hawthorn (Crataegus spp.) is one of the most famous plants which has been used as natural medicine and nutraceutical. Its phytochemical composition, bioactive compounds and health benefits have been intensively studied and hawthorn preparations may be recognized as classical natural products for cardiovascular health. Polyphenolic compounds of different hawthorn anatomical parts as well as their extracts have been the focus of a majority of these studies, although various other classes of natural health promoting constituents have also been isolated, identified and characterized. Regardless, numerous published reports have particularly focused on the activity mechanisms which are very important for supporting various health benefits. This review summarizes the most recent studies on hawthorn, mainly published since 2015. Search of different databases indicates that approximately 200 publications, which are relevant to phytochemistry and health benefits of Crataegus spp., have appeared since then, most of them have not been included in the existing reviews.

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Section: Venskutonismentioning

confidence: 86%

Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): review of recent research advances

Venskutonis¹

2018

JFB

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“…Since PI3K/Akt/mTOR and MEK/Erk signal pathways are the key axis involved in autophagy regulation by anticancer drugs (Ciuffreda et al , 2010, we further investigated the effects of ADM on the activation of the signal molecules Akt and p70S6K in PI3K/Akt/mTOR cascade and Erk1/2 in MEK/Erk cascade (Aoki et al , 2007, Fu et al , 2016, Hennessy et al , 2005, Jung et al , 2010, Laplante and Sabatini, 2009. As shown in Figure 2B，ADM dose-dependently inhibited the basal activation levels of Akt (T308) and p70S6K (T389) in TRAMP-C2 and LNCaP cells, whereas no alteration of p-Erk1/2 (T202/Y204) level was found in cells treated with ADM.…”

Section: Adm Induces Autophagy Via Pi3k/akt(t308)/mtor Cascadementioning

confidence: 99%

Inhibition of autophagy promotes apoptosis and enhances anticancer efficacy of adriamycin via augmented ROS generation in prostate cancer cells

Wang

Tan

Yang

et al. 2016

The International Journal of Biochemistry & Cell Biology

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The interplay between autophagy and apoptosis response to chemotherapy is still a subject of intense debate in recent years. More efforts have focused on the regulation effects of apoptosis on autophagy, whereas how autophagy affects apoptosis remains poorly understood. In this study performed on prostate cancer cells, we investigated the role of autophagy in adriamycin-induced apoptosis, as well as the mechanisms mediating the effects of autophagy on apoptosis response to adriamycin (ADM). The results show that ADM not only inhibited cell viability and enhanced apoptosis, but also promoted autophagy via PI3K/Akt(T308)/mTOR signal pathway. Inhibition of autophagy by either pharmacological inhibitor chloroquine (CQ) or RNA interference of Atg5 increased ADM-induced apoptosis and enhanced the chemosensitivity of prostate cancer cells. Moreover, blockade of autophagy augmented reactive oxygen species (ROS) generation induced by ADM. Scavenging of ROS by antioxidant N-acetyl-cysteine (NAC) reversed the strengthened effects of CQ on ADM-induced apoptosis and rescued the cells from apoptosis. The results identified ROS as a potential mediator directing the modulation effects of the protective autophagy on apoptosis response to ADM. Suppression of the protective autophagy might provide a promising strategy to increase the anticancer efficacy of agents in the treatment of prostate cancer.

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“…Flavonoids are the most common and abundant polyphenols humans are typically exposed to in the course of normal daily life and have a wide range of pharmacological effects (Fu et al, 2016). In vitro studies have shown that anti-cancer effects of flavonoids may be related to their induction of inhibition of: cell proliferation, cell adhesion, cell invasion, cell differentiation, cell cycle phases, and cell apoptosis (Yan et al, 2017;Zhang et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

Sotetsuflavone Induces Autophagy in Non-Small Cell Lung Cancer Through Blocking PI3K/Akt/mTOR Signaling Pathway in Vivo and in Vitro

Wang

et al. 2019

Front. Pharmacol.

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Non-small cell lung cancer (NSCLC) is a globally scaled disease with a high incidence and high associated mortality rate. Autophagy is one of the important physiological activities that helps to control cell survival, influences the dynamics of cell death, and which plays a crucial role in the pathophysiology of NSCLC. Sotetsuflavone is a naturally derived and occurring flavonoid, and previous studies have demonstrated that sotetsuflavone possesses potential anti-cancer activities. However, whether or not sotetsuflavone induces autophagy, as well as has effects and influences cell death in NSCLC cells remains unclear. Thus, in our study, we examined and elucidated the roles and underlying mechanisms of sotetsuflavone upon the dynamics of autophagy in NSCLC in vivo and in vitro. The results indicated that sotetsuflavone was able to inhibit proliferation, migration, and invasion of NSCLC cells. Mechanistically, sotetsuflavone was able to induce apoptosis by increasing the levels of expression of cytochrome C, cleaved-caspase 3, cleaved-caspase 9, and Bax, and contrastingly decreased levels of expression of Bcl-2. In addition, we also found that decreased levels of expression of cyclin D1 and CDK4 caused arrest of the G0/G1 phases of the cell cycle. Furthermore, we also found that sotetsuflavone could induce autophagy which in turn can play a cytoprotective effect on apoptosis in NSCLC. Sotetsuflavone-induced autophagy appeared related to the blocking of the PI3K/Akt/ mTOR pathway. Our in vivo study demonstrated that sotetsuflavone significantly inhibited the growth of xenograft model inoculated A549 tumor with high a degree of safety. Taken together, these findings suggest that sotetsuflavone induces autophagy in NSCLC cells through its effects upon blocking of the PI3K/Akt/mTOR signaling pathways. Our study may provide a theoretical basis for future clinical applications of sotetsuflavone and its use as a chemotherapeutic agent for treatment of NSCLC.

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Hyperoside induces both autophagy and apoptosis in non-small cell lung cancer cells in vitro

Cited by 51 publications

References 55 publications

Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): review of recent research advances

Phytochemical composition and bioactivities of hawthorn (Crataegus spp.): review of recent research advances

Inhibition of autophagy promotes apoptosis and enhances anticancer efficacy of adriamycin via augmented ROS generation in prostate cancer cells

Sotetsuflavone Induces Autophagy in Non-Small Cell Lung Cancer Through Blocking PI3K/Akt/mTOR Signaling Pathway in Vivo and in Vitro

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