Hyper-osmotic condition enhances protein tyrosine phosphorylation and zona pellucida binding capacity of human sperm

Liu, D.Y.; Clarke, Gary N.; Baker, H. W. G.

doi:10.1093/humrep/dei364

Cited by 18 publications

(19 citation statements)

References 26 publications

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“…Colone et al show that MYO administration could help to reduce the presence of this amorphous material [37]. MYO has also a crucial role in the osmoregulation of seminal fluid and as a consequence in sperm progressive motility and velocities [38]. Gulino et al investigate the effect of MYO administration on semen parameters of male patients undergoing IVF cycles.…”

Section: Inositol In Sperm Cellmentioning

confidence: 99%

Inositol and In Vitro Fertilization with Embryo Transfer

Simi

Genazzani

Obino

et al. 2017

International Journal of Endocrinology

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Recently, studies on inositol supplementation during in vitro fertilization program (IVF) have gained particular importance due to the effect of this molecule on reducing insulin resistance improving ovarian function, oocyte quality, and embryo and pregnancy rates and reducing gonadotropin amount during stimulation. Inositol and its isoforms, especially myoinositol (MYO), are often used as prestimulation therapy in infertile patients undergoing IVF cycle. Inositol supplementation started three months before ovarian stimulation, resulting in significant improvements in hormonal responses, reducing the amount of FSH necessary for optimal follicle development and serum levels of 17beta-estradiol measured the day of hCG injection. As shown by growing number of trials, MYO supplementation improves oocyte quality by reducing the number of degenerated and immature oocytes, in this way increasing the quality of embryos produced. Inositol can also improve the quality of sperm parameters in those patients affected by oligoasthenoteratozoospermia.

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Section: Inositol In Sperm Cellmentioning

confidence: 99%

Inositol and In Vitro Fertilization with Embryo Transfer

Simi

Genazzani

Obino

et al. 2017

International Journal of Endocrinology

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“…It is known that hyper-osmotic medium can inhibit the A23187-induced AR of human sperm (Bielfeld et al, 1993). Our preliminary tests have shown that hyperosmotic medium may also block the ZP-induced AR of sperm bound to the ZP (Liu et al, 2005). Motile sperm from 10 normospermic men were incubated for 2 h with 4 oocytes in hyper-osmotic medium (400 mOsm/kg, AR AI 10 µm adjusted by addition of extra of NaCl to HTF medium) and control (iso-osmotic, 280 mOsm/L) medium.…”

Section: Experiments III -Hyper-osmotic Mediummentioning

confidence: 68%

“…, 1993). Our preliminary tests have shown that hyper‐osmotic medium may also block the ZP‐induced AR of sperm bound to the ZP (Liu et al. , 2005).…”

Section: Methodsmentioning

confidence: 97%

Acrosome‐reacted human sperm in insemination medium do not bind to the zona pellucida of human oocytes

2006

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In the literature there is still confusion whether acrosome-reacted sperm in medium can initiate primary binding to human zona pellucida (ZP). The ability of acrosome-reacted sperm to bind to ZP in vitro can be deduced by measuring the acrosome reaction (AR) of ZP-bound sperm compared with sperm in medium after incubation under different conditions inhibiting the ZP-induced AR. Motile sperm from fertile men, normospermic men and infertile men diagnosed with disordered ZP-induced AR (DZPIAR) were selected by swim-up (2 x 10(6) in 1 mL medium) and incubated for 1-2 h with four oocytes from failed in vitro fertilization (IVF). The acrosome status of sperm was assessed using pisum sativum agglutinin labelled with fluorescein. The ZP-induced AR was inhibited in experiments using sperm from DZPIAR patients, hyper-osmotic medium (400 mOsm/kg) and medium containing soybean trypsin inhibitor (SBTI; 4 mg/mL). Pre-treatment with calcium ionophore was used to create a sperm population with elevated AR. In all experiments with factors inhibiting the ZP-induced AR, the AR was significantly lower for ZP-bound sperm compared with sperm in medium: DZPIAR patients 4% vs. 15%, hyper-osmotic medium 3% vs. 12%, SBTI 2% vs. 12% and SBTI 3% vs. 23% after treatment with calcium ionophore. In conclusion, acrosome-reacted sperm in vitro have significantly reduced, in fact probably zero ability to bind to the ZP.

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“…Known effects of osmotic stress conditions on sperm include the production of oxygen free radicals, which leads to the peroxidation of membrane lipids (Alvarez and Storey, 1992; McCarthy et al, 2010), a decrease in progressive (forward) and total motility, an increase in the tyrosine phosphorylation of tail proteins (Liu et al, 2006), and a change in the actin cytoskeleton (Correa et al, 2007). All of these changes, to some degree, are also altered during the membrane changes that occur during capacitation.…”

Section: Discussionmentioning

confidence: 99%

Osmotic Stress Stimulates Phosphorylation and Cellular Expression of Heat Shock Proteins in Rhesus Macaque Sperm

Cole

Meyers

2010

Journal of Andrology

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The cryosurvival of sperm requires cell signaling mechanisms to adapt to anisotonic conditions during the freezing and thawing process. Chaperone proteins heat shock protein 70 (HSP 70) and heat shock protein 90 (HSP 90; recently renamed HSPA and HSPC, respectively) facilitate some of these cell signaling events in somatic cells. Sperm were evaluated for their cellular expression and levels of phosphorylation of both HSP 70 and HSP 90 under anisotonic conditions as a potential model for cell signaling during the cryopreservation of macaque spermatozoa. In order to monitor the level of stress, the motility and viability parameters were evaluated at various time points. Cells were then either prepared for phosphoprotein enrichment or indirect immunocytochemistry. As controls, the phosphoserine, phosphothreonine, and phosphotyrosine levels were measured under capacitation and cryopreservation conditions and were compared with the phosphoprotein levels expressed under osmotic conditions. As expected, there was an increase in the level of tyrosine phosphorylation under capacitation and cryopreservation conditions. There was also a significant increase in the level of all phosphoproteins under hyperosmotic conditions. There was no change in the level of expression of HSP 70 or 90 under osmotic stress conditions as measured by Western blot. The enrichment of phosphoproteins followed by Western immunoblotting revealed an increase in the phosphorylation of HSP 70 but not HSP 90 under osmotic stress conditions. Indirect immunofluorescence localized HSP 70 to the postacrosomal region of sperm, and the level of membrane expression of HSP 70 was significantly affected by anisotonic conditions, as measured by flow cytometry. Taken together, these results suggest a differential role for HSP 70 and HSP 90 during osmotic stress conditions in rhesus macaque sperm.

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Hyper-osmotic condition enhances protein tyrosine phosphorylation and zona pellucida binding capacity of human sperm

Abstract: BACKGROUND: The aim of this study was to determine the effect of culture medium osmolality, in the range known to occur in the male and female reproductive tracts, on human sperm tyrosine phosphorylation and sperm-zona pellucida (ZP) interaction in vitro. METHODS: Motile sperm (2ϫ10

Cited by 18 publications

References 26 publications

Inositol and In Vitro Fertilization with Embryo Transfer

Inositol and In Vitro Fertilization with Embryo Transfer

Acrosome‐reacted human sperm in insemination medium do not bind to the zona pellucida of human oocytes

Osmotic Stress Stimulates Phosphorylation and Cellular Expression of Heat Shock Proteins in Rhesus Macaque Sperm

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