Hyoscyamine 6β-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root

Hashimoto, Takashi; Hayashi, Asami; Amano, Y.; Kohno, Junko; Iwanari, Hiroko; Usuda, Sadakazu; Yamada, Yasuyuki

doi:10.1016/s0021-9258(20)64371-x

Cited by 171 publications

(23 citation statements)

References 11 publications

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“…The content of secondary metabolites in the different organs showed some positive correlation. The high TAs content in roots and its positive correlation is in agreement with Hashimoto, who found the biosynthesis of TAs takes place in the roots, from where they are transported to the aerial parts of the plants [20].…”

Section: Concentrations Of Tas From Different Geographic Originssupporting

confidence: 89%

Multivariate statistical analysis of tropane alkaloids in Anisodus tanguticus (Maxim.) Pascher from different regions to trace geographical origins

Chen

Xiong

et al. 2022

AChrom

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Anisodus tanguticus (Maxim.) Pascher is an important Tibetan folk medicine and the source of tropane alkaloids (TAs) grown in Qinghai-Tibet Plateau. There are marked differences in quality of A. tanguticus from geographic areas. The aim of present research was to establish a method for the quantitative analysis of TAs coupled with chemometrics analysis to trace geographical origins. Qualitative analysis of TAs in A. tanguticus was carried out using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and quantitative analysis of TAs in different plant organs from different geographical origin was achieved. Contents of TAs were subjected to the principal component analysis, and orthogonal partial least-squares discriminant analysis. The contents of the three marker compounds (anisodamine, anisodine and atropine) in the roots and acrial parts of A. tanguticus were positive correlated and varied significantly from different geographical origins. Principal component analysis, and orthogonal partial least-squares discriminant analysis results showed excellent discrimination between different geographical origin of A. tanguticus. This study could provide comprehensive evaluation and further utilization of A. tanguticus resources.

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Section: Concentrations Of Tas From Different Geographic Originssupporting

confidence: 89%

Multivariate statistical analysis of tropane alkaloids in Anisodus tanguticus (Maxim.) Pascher from different regions to trace geographical origins

Chen

Xiong

et al. 2022

AChrom

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“…As an example, Hyoscyamine biosynthesis in Hyoscyamus niger takes place in the root pericycle. Through immunohistochemical assay, the accumulation of 6β-hydroxylase (H6H) that catalyzes the reaction of hyoscyamine to scopolamine is found only in intact root and root culture; from this root, the product scopolamine is transported to the aerial part via xylem or phloem (Hashimoto et al 1991).…”

Section: Sites Of Secondary Metabolites Biosynthesis and Accumulationmentioning

confidence: 99%

Localization of Alkaloid and Other Secondary Metabolites in Cinchona ledgeriana Moens: Anatomical and Histochemical Studies on Fresh Tissues and Cultured Cells

2020

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Cinchona ledgeriana produces several secondary metabolites. The main quinoline alkaloid, quinine that is widely used as an antimalarial drug, is most commonly extracted from the bark of Cinchona, and its leaves contain several other metabolites. Many studies have revealed that cell culture of Cinchona also produces quinine. Nevertheless, the sites of secondary metabolites accumulation are still elusive. This study is aimed at describing specific anatomical structures where alkaloids and some other secondary metabolites are accumulated as well as their localization in leaves and barks of C. ledgeriana, compared to those found in cultured cells. Fresh leaves and barks, and cells of C. ledgeriana were used for anatomical observation and histochemical tests. It was found that these plant parts have specialized structures, idioblast cells with elliptical- and spherical-shapes, scattered in leaf hypodermis, stem cortex, and secondary phloem. Unspecialized structures such as epidermis and palisade mesophyll tissues were also found accumulating some metabolites. Histochemical tests showed that bark and leaves contained alkaloids, terpenoids, phenolic, and lipophilic compounds. Cultured cells presented positive results for alkaloids and terpenoids.

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“…In some polygonaceous species, such as Rheum emodi , anthranoids were also detected in leaves ( Pandith et al 2014 ). In scopolamine producing Atropa belladonna and Hyoscyamus niger , putrescine N -methyltransferase and hyoscyamine 6 � -hydroxylase, which catalyze the first and last biosynthetic steps, respectively, were localized to the pericycle, mainly to the cells adjacent to the primary xylem poles ( Hashimoto et al 1991 , Kanegae et al 1994 , Suzuki et al 1999a , Suzuki et al 1999b ). The xylem was reported to translocate tropane alkaloids, such as scopolamine, from the roots to the shoot ( Neumann 1985 ).…”

Section: Discussionmentioning

confidence: 99%

Octaketide Synthase from Polygonum cuspidatum Implements Emodin Biosynthesis in Arabidopsis thaliana

Guo

Nassar

et al. 2021

Plant and Cell Physiology

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Hyoscyamine 6β-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root

Cited by 171 publications

References 11 publications

Multivariate statistical analysis of tropane alkaloids in Anisodus tanguticus (Maxim.) Pascher from different regions to trace geographical origins

Multivariate statistical analysis of tropane alkaloids in Anisodus tanguticus (Maxim.) Pascher from different regions to trace geographical origins

Localization of Alkaloid and Other Secondary Metabolites in Cinchona ledgeriana Moens: Anatomical and Histochemical Studies on Fresh Tissues and Cultured Cells

Octaketide Synthase from Polygonum cuspidatum Implements Emodin Biosynthesis in Arabidopsis thaliana

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