The effective, energy-saving and green subcritical fluid extraction (SFE) technology was applied to obtain the oil from Lycium ruthenicum seeds (LRSO). The optimal conditions of extraction parameters were found using response surface methodology with Box-Behnken experimental design. The maximum extraction yield of 21.20% was achieved at raw material particle size of 0.60 mm, extraction pressure of 0.63 MPa, temperature of 50°C and time of 48 min. Other traditional extraction technologies were comparatively used. The physicochemical property of LRSO was analysed and the chemical compositions indicated that they were rich in unsaturated fatty acid, b-carotene, tocopherols and total phenolics. Furthermore, the antioxidant activity of LRSO was evaluated by scavenging activity of three kinds of radicals (DPPHÁ, ÁOH and O 2 -Á) and lipid peroxidation in vitro. And its results showed the oil had the potential to be a novel antioxidant agent for using in the field of food, pharmaceuticals and cosmetics. The study of Lycium ruthenicum seeds oil Z. Liu et al. The study of Lycium ruthenicum seeds oil Z. Liu et al. † The LRSO was obtained by subcritical fluid extraction. The values of contents are means AE SD (n = 3). Cele, Xinjiang Province versus other regions. * P < 0.05. ** P < 0.01.The study of Lycium ruthenicum seeds oil Z. Liu et al.
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