Localization of Alkaloid and Other Secondary Metabolites in Cinchona ledgeriana Moens: Anatomical and Histochemical Studies on Fresh Tissues and Cultured Cells

Pratiwi, Dian Rahma; Sulistyaningsih, Yohana Caecilia; Ratnadewi, Diah

doi:10.4308/hjb.27.1.1

Cited by 4 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to being found in the cell walls of the xylem constituents in roots and leaves, terpenoid compounds in roots are more commonly found in the form of idioblast structures distributed in the root cortex tissue. The same finding was also found in undifferentiated cells in Cinchona ledgeriana Moens culture which was observed for terpenoid compounds using 5% CuSO4 reagent (Pratiwi et al, 2020). In leaves, terpenoids are also found in the leaf cuticle because the cuticle is composed of terpenoid and flavonoid compounds that are involved in defense mechanisms from biotic and abiotic (Singh et al, 2018;Ziv et al, 2018).…”

Section: Histochemical Observationsupporting

confidence: 54%

Anatomical and Histochemical Responses of Vetiver Grass (Chrysopogon zizanioides L. Roberty) to Phytoremediation Ability of Liquid Batik Waste

Linggawati¹,

Maryani²,

Nugroho³

et al. 2022

Environ. Nat. Resour. J.

View full text Add to dashboard Cite

Due to poor management of Indonesian batik waste, pollutants are discharged directly into rivers and absorbed from soil, causing environmental pollution. A phytoremediation strategy was chosen as one of the environmentally friendly and community-implementable solutions. Vetiver grass (Chrysopogon zizanioides L. Roberty) is a type of Poaceae plant that is suitable for the phytoremediation process of batik waste. This study analyzed the anatomical responses, distribution of secondary metabolites as defense compounds, and the ability to absorb heavy metals contained in liquid batik waste. Liquid batik waste was applied as plants irrigation at various concentration (0%, 25%, 50%, 75%, and 100%) for 60 days. Vetiver grass was able to grow well in the applied concentrations range. The results showed that vetiver grass roots could absorb Cu metal better than in the leaves. This plant can be stated to be able to absorb Cu better than Al. Liquid batik waste significantly (p<0.05) affected most of the observed anatomical parameters, where concentrations of 75% and 100% were the most influential concentrations according to the DMRT test with a 95% confidence level. The histochemical analysis found that there was an increase in the distribution of lignin, phenolic compound, and terpenoids in the tissues composed of roots and leaves along with the increase in the concentration of the waste applied.

show abstract

Section: Histochemical Observationsupporting

confidence: 54%

Anatomical and Histochemical Responses of Vetiver Grass (Chrysopogon zizanioides L. Roberty) to Phytoremediation Ability of Liquid Batik Waste

Linggawati¹,

Maryani²,

Nugroho³

et al. 2022

Environ. Nat. Resour. J.

View full text Add to dashboard Cite

show abstract

“…In both the leaf lamina and petioles, parenchyma cells that took up the stain were present in palisade and spongy parenchyma cells, the cortex and pith. In the same manner, localization of alkaloids in Cinchona ledgeriana was shown in the idioblasts in the leaf hypodermis and leaf palisade [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Seasonal and Geographic Variation in Alkaloid Content of Kratom (Mitragyna speciosa (Korth.) Havil.) from Thailand

Sengnon

Vonghirundecha

Chaichan³

et al. 2023

Plants

View full text Add to dashboard Cite

The objective of this study was to obtain data on the distribution of alkaloids in kratom plants grown in Thailand. Two collections were performed, covering the southern, central, and northern regions of Thailand and different seasons. The contents of alkaloids, including mitragynine (MG), paynantheine (PAY), and speciogynine (SG), were determined using the validated HPLC method. The 134 samples in the first collection were collected from Nam Phu subdistrict, Ban Na San, Surat Thani, Thailand, during June and October 2019 and January 2020. The maximum mitragynine content was 4.94% w/w in June (late summer), and the minimum content was 0.74% w/w in October (rainy season). To expand the study area after kratom decriminalization, 611 samples were collected in June–August 2021, October–December 2021, and January–April 2022. The accumulation of MG ranged from 0.35 to 3.46% w/w, 0.31 to 2.54% w/w, and 0.48 to 2.81% w/w, respectively. The meteorological data supported the climate’s effect on alkaloid production. Soil analysis revealed the importance of Ca and Mg in promoting alkaloid production. Geographical locations played a role in the variation of MG in kratom leaves, but did not affect the color of leaf veins. In conclusion, the present study suggested that the alkaloid content in kratom diverges based on seasonal and geographical origin.

show abstract

“…Each segment was soaked in citric acid:boric (Prolabo) (5:5 w/w) in 100 mL absolute ethanol for 15 minutes, mounted in glycerine water and inspected under a light microscope (Olympus, Tokyo, Japan). A yellow color signifies the presence of flavonoids (Pratiwi et al 2020). With 5% copper acetate (Cu2(CH3COO)4), terpenoids stained yellow-brown, indicating their presence in cells or tissues (Pratiwi et al 2020).…”

Section: Histochemical Testmentioning

confidence: 99%

“…A yellow color signifies the presence of flavonoids (Pratiwi et al 2020). With 5% copper acetate (Cu2(CH3COO)4), terpenoids stained yellow-brown, indicating their presence in cells or tissues (Pratiwi et al 2020). FeCl3 (0.5% -1% FeCl3 in 0.1 N HCL) stained tannins blackish green, suggesting their existence (Robil and Tolentino 2015).…”

Section: Histochemical Testmentioning

confidence: 99%

“…FeCl3 (0.5% -1% FeCl3 in 0.1 N HCL) stained tannins blackish green, suggesting their existence (Robil and Tolentino 2015). Wagner's reagent was used to detect alkaloids, and any red-brown staining of tissues or cells indicated the presence of alkaloid compounds (Pratiwi et al 2020). The material was photographed using OptiLab and a light microscope (Olympus, Tokyo, Japan).…”

Section: Histochemical Testmentioning

confidence: 99%

See 1 more Smart Citation

The morphoanatomy, histochemistry, and phytochemistry of the leaves and fruits of Rhodomyrtus tomentosa

2023

View full text Add to dashboard Cite

Abstract. Kuntorini EM, Sari SG, Fariani R. 2023. The morphoanatomy, histochemistry, and phytochemistry of the leaves and fruits of Rhodomyrtus tomentosa. Biodiversitas 24: 98-105. Rhodomyrtus tomentosa (Ait.) Hassk is native to Southeast Asia. The entire plant has been used in traditional Vietnamese, Chinese, and Malaysian medicine for a long time. Bioactive substances are abundant in ripe fruits. However, few studies have examined phytochemical changes and distribution throughout leaf and fruit growth. This study evaluated the morphoanatomy, histochemical, and phytochemical properties of R. tomentosa leaves and fruits. The cross-sectional anatomical structures observed in the leaves included the epidermis, mesophyll, and carrier bundle, while the fruit structures observed included a pericarp layer with an exocarp/outer layer, mesocarp, and endocarp. Flavonoids, tannins, terpenoids, and alkaloids were found in the leaf's adaxial and abaxial epidermis, mesophyll, xylem, phloem, midrib parenchyma, secretory cavities, and trichomes. They were also distributed throughout the fruit's exocarp, mesocarp, endocarp, secretory cavities, xylem, phloem, trichomes, and seeds. Young leaves contained the highest total flavonoid concentration (196 ± 1.77 mg QE/g), green fruit had the highest total phenol concentration (97.70 ± 18.15 mg GAE/g), old leaves contained the highest total alkaloid concentration (13.22 ± 0.98%), and red fruit had the highest total tannin concentration (1.66 ± 0.15 mg GAE/g).

show abstract

Localization of Alkaloid and Other Secondary Metabolites in Cinchona ledgeriana Moens: Anatomical and Histochemical Studies on Fresh Tissues and Cultured Cells

Cited by 4 publications

References 23 publications

Anatomical and Histochemical Responses of Vetiver Grass (Chrysopogon zizanioides L. Roberty) to Phytoremediation Ability of Liquid Batik Waste

Anatomical and Histochemical Responses of Vetiver Grass (Chrysopogon zizanioides L. Roberty) to Phytoremediation Ability of Liquid Batik Waste

Seasonal and Geographic Variation in Alkaloid Content of Kratom (Mitragyna speciosa (Korth.) Havil.) from Thailand

The morphoanatomy, histochemistry, and phytochemistry of the leaves and fruits of Rhodomyrtus tomentosa

Contact Info

Product

Resources

About