The electroplating industry generates wastewater containing a variety of heavy metals which potentially contaminate water ecosystems. The available and well-known electroplating wastewater treatments are considered as an expensive and less effective method, therefore phytoremediation was used as an alternative friendly solution. This study aims to evaluate the uptake and elimination rate of heavy metals by vetiver (Chrysopogon zizanoides L.) on metal-polluted water. Vetiver was planted in artificial electroplating wastewater containing different levels (low, medium, high) of chromium (Cr) and nickel (Ni). Water, roots, and shoots were collected periodically to determine Cr and Ni contents using Atomic Absorption Spectrometry (AAS). Metal accumulation and elimination rate, Bioconcentration Factor (BCF), Biological Absorption Coefficient (BAC), and Translocation Factor (TF) were calculated to evaluate plant’s effectiveness in metal remediation processes. The results showed that vetiver (C. zizanoides L.) was able to remove 61.10% Cr and 95.65% Ni on metal-contaminated water. The highest uptake rates for Cr and Ni are 127.21 mg/kg/day and 15.60 mg/kg/day respectively, while the elimination rates for Cr and Ni tend to slow 1.09 mg/kg/day and 12.24 mg/kg/day respectively. Vetiver BCF, BAC, and TF values on Cr and Ni contaminated water were greater than 1, which indicates that vetiver work through phytoextraction and phytostabilization to treat metals. The findings showed that vetiver has promise as a phytoremediation agent thus providing implication for electroplating wastewater treatment.

Chitosan is a derivative compound of chitin which has a linear polysaccharide composed of β-(1-4)-linked d-glucosamine and N-acetyl-d-glucosamine. This compound is found in shrimp shell. In nanoparticle form, chitosan has a great antibacterial activity and adsorption ability rather than normal form. The aims of this study are to study the effect of concentration chitosan nanoparticle to adsorpt cadmium and its antibacterial effect on coliform. This research was started with synthesis of chitosan nanoparticles using acetic acid 2% and TPP 0,1 %, then the sampel was dried by spray dryer. For cadmium adsorption test was conducted by giving chitosan nanoparticle of 0.1, 0.2, 0.3, 0.4, and 0.5 g, respectively, into Cd solution with the concentration of 7 ppm and was filtered with theWhatman paper number 42 (n=3). Cadmium contents in the filtrates and pellets were analyzed by using AAS. For antibacterial test with Salmonella typhimurium andEscherichia coliwere conducted by disc diffusion method, contained of 0.1, 0.2, 0.3, 0.4, and 0.5 g, respectively, were dissolved in 50 ml of 1% acetic acid. Data were analyzed by using oneway ANOVA followed by LSD. Based on the results, the optimum adsorption of cadmium present in addition of 0.4 g/ 50mL, can reduce Cd concentration by 98,7%. For the antibacterial test, the addition of 0.4 g/50mL is the largest zone of inhibition Salmonella thypimuriumand Escherichia coli.Keywords: antibacterial coliform, cadmium adsorbent, chitosan nanoparticle INTISARI Kitosan merupakan senyawa derivat dari kitin yang memiliki linear polisakarida yang tersusun dari β-(1-4)-linked d-glucosamine dan N-acetyl-d-glucosamine. Senyawa ini banyak terkandung dalam kulit udang. Dalam bentuk nanopartikel, kitosan memiliki aktivitas antibakteri dan adsorpsi yang lebih baik jika dibandingkan dalam bentuk biasa. Tujuan penelitian ini adalah untuk mempelajari pengaruh konsentrasi kitosan nanopartikel terhadap pengikatan cadmium (Cd) dan efeknya pada aktivitas antibakteri koliform. Penelitian ini dimulai dengan sintesis senyawa nanopartikel kitosan dengan larutan asam cuka 2% dan TPP 0,1, kemudian larutan dikeringkan dengan spray drier. Uji adsorpsi logam cadmium dilakukan dengan menambahkan 0,1; 0,2; 0,3; 0,4 dan 0,5 gram nanopartikel kitosan ke dalam larutan cadmium dengan konsentrasi 7 ppm,dan disaring dengan kertas Whatman no 42 (n=3). Kandungan Cd dalam filtrat dan residu ditentukan dengan AAS. Uji antibakteri terhadap Salmonella typhimurium dan Escherichia coli dilakukan dengan metode cakram, yang mengandung 0,1; 0,2; 0,3; 0,4 dan 0,5 gram nanopartikel kitosan dalam 50 ml asam cuka 1%. Data kemudian dianalisis dengan oneway ANOVA dilanjutkan dengan LSD, apabila ditemukan beda nyata. Hasil yang diperoleh menunjukkan bahwa pengikatan optimum Cd terjadi pada penambahan kitosan sebanyak 0,4 g/50 mL asam cuka, dengan penurunan sebesar 98,7%. Pada uji antibakteri, penambahan kitosan 0,4 g/50 mL asam cuka menunjukkan penghambatan tertinggi terhadap pertumbuhan S. typhimurium dan E. coli.Kata kunci: adsor...

The effectiveness of using Vetiver grass (Chrysopogon zizanioides) in phytoremediation of wastewater has been proven. In this study, the phytoremediation potential of C. zizanioides planted in Cr- and Ni-contaminated soil was evaluated through investigating the behaviors on uptake and release of metals. Three treatments: control, Cr, and Ni, with three concentrations (50, 150, and 300 ppm), were applied. The potential of C. zizanioides is assessed by the determination of metal uptake rate, metal release rate, bioconcentration factor (BCF), biological absorption coefficient (BAC), and translocation factor (TF). The experiment showed that Cr uptake was higher than release rate and on the other hand low in uptake and release of Ni. Accumulation of Cr and Ni was 167.8 mg kg−1 and 66.3 mg kg−1, respectively. Excess of Cr in the soil was absorbed in high uptake rate making vetiver grass suitable for Cr phytoremediation. During 28-day uptake and 28-day release periods, it was found that BCF, BAC, and TF values in some treatments showed greater than 1 (one) and Ni-treated plants were able to translocate Ni to aerial plant parts supported by its high TF value. Low acidity of soil causes low solubility and low mobility of metals, resulting in low metal absorption. C. zizanioides has shown the potential as a heavy metal-tolerant species and could be potentially used as phytoremediation alternative species at least in lightly polluted areas.

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Media and pH are two crucial factors in microalgal cultivation. Industrial wastewater such as tofu wastewater can be utilized as alternative media for growing microalgae like Euglena sp. to produce biomass as feedstock in biorefinery activities. Here, we evaluated combinations of tofu wastewater (L) consisting of 0% (L1), 75% (L2), and 100% (L3) with pH (P) levels consisting of 5.0 (P1), 5.5 (P2), 6.0 (P3), 6.5 (P4), and 7.0 (P5). The analyses were carried out on the growth kinetics, biomass, primary metabolite compounds, and pigments of Euglena sp. Based on the study, the combinations with the highest cell density, biomass, maximum carbohydrate content, maximum lipid content, and protein content were L2P2 (23.13x105 cells/ml), L2P1 (4.53±0.17 mg/ml), L1P5 (0.93±0.02 mg/ml), L2P1 (1.27±0.11 mg/ml), and L3P4 (256±26.86 ppm), respectively. Moreover, the combinations with the highest chlorophyll-a, chlorophyll-b, and carotenoid were L2P4 (33.53±0.13 mg/l), L2P2 (17.73±0.50 mg/l), and L2P2 (11.65±0.00 mg/l), respectively. The addition of tofu wastewater combined with specific pH level enhanced the growth and biomass composition of Euglena sp. (P<0.05), with the exception of carbohydrate content. Additionally, each biochemical component of Euglena sp. had a different optimum combination of tofu wastewater and pH level. However, this wastewater can potentially be used as an alternative medium for cultivating this microalga in order to cut the production costs of biorefinery activity.

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