Uptake, distribution, and bioaccumulation of copper in the freshwater mussel<i>Anodonta anatina</i>

Nugroho, Andhika Puspito; Frank, Hartmut

doi:10.1080/02772248.2011.582989

Cited by 11 publications

(7 citation statements)

References 19 publications

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“…Different patterns of Cu uptake and tissue distribution have been reported for various bivalve species and exposure conditions (Canesi et al 1999;García-Navarro et al 2017;Nugroho and Frank 2011;Sakellari et al 2013;Serafim and Bebianno 2009;Won et al 2016). Whole-body BCFs of 3300 L/kg WW (Potipat et al 2015) and 576 to approximately Fig.…”

Section: Cu Exposure and Uptakementioning

confidence: 99%

“…Gills constitute a first-line defense against harmful toxicants, and gill uptake might be of importance for bivalve regulation of reactive oxygen species (ROS) under Cu stress (Won et al 2016). However, fecal elimination, via the digestive gland, has been proposed as a major route for metal elimination in A. anatina (Nugroho and Frank 2011). At low exposure concentrations, an efficient Cu elimination might thus result in no net uptake to digestive glands.…”

Section: Cu Exposure and Uptakementioning

confidence: 99%

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Transcriptional and biochemical biomarker responses in a freshwater mussel (Anodonta anatina) under environmentally relevant Cu exposure

Ugge

Jonsson

Olsson

et al. 2020

Environ Sci Pollut Res

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Molecular biomarkers, like gene transcripts or enzyme activities, are potentially powerful tools for early warning assessment of pollution. However, a thorough understanding of response and baseline variation is required to distinguish actual effects from pollution. Here, we assess the freshwater mussel Anodonta anatina as a biomarker model species for freshwater ecosystems, by testing responses of six transcriptional (cat, gst, hsp70, hsp90, mt, and sod) and two biochemical (AChE and GST) biomarkers to environmentally relevant Cu water concentrations. Mussels (n = 20), collected from a stream free from point source pollution, were exposed in the laboratory, for 96 h, to Cu treatments (< 0.2 μg/L, 0.77 ± 0.87 μg/L, and 6.3 ± 5.4 μg/L). Gills and digestive glands were extracted and analyzed for transcriptional and biochemical responses. Biological and statistical effect sizes from Cu treatments were in general small (mean log 2 fold-change ≤ 0.80 and Cohen's f ≤ 0.69, respectively), and no significant treatment effects were observed. In contrast, four out of eight biomarkers (cat, gst, hsp70, and GST) showed a significant sex:tissue interaction, and additionally one (sod) showed significant overall effects from sex. Specifically, three markers in gills (cat, mt, GST) and one in digestive gland (AChE) displayed significant sex differences, independent of treatment. Results suggest that sex or tissue effects might obscure low-magnitude biomarker responses and potential early warnings. Thus, variation in biomarker baselines and response patterns needs to be further addressed for the future use of A. anatina as a biomarker model species.

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Section: Cu Exposure and Uptakementioning

confidence: 99%

Section: Cu Exposure and Uptakementioning

confidence: 99%

Transcriptional and biochemical biomarker responses in a freshwater mussel (Anodonta anatina) under environmentally relevant Cu exposure

Ugge

Jonsson

Olsson

et al. 2020

Environ Sci Pollut Res

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“…Filter feeding would thus be a potential exposure route of metals associated with algal cells or present in particulate forms (Hull et al 2013 ; Lee et al 2015 ). Considering the static exposure and moderate concentrations of each separate metal, elimination rates after 96 h might have been high enough to balance potential uptake (King et al 2005 ; Nugroho and Frank 2011 ). Another potential explanation is that the effluent might trigger avoidance behavior to reduce the actual exposure (Hartmann et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…Bivalves are likely exposed to toxicants occurring in their (natural or laboratory) environment due to sessility and filtration feeding, and A. anatina could serve as an ecologically relevant freshwater model in ecotoxicology. Previous studies cover, e.g., pollutant uptake (Berglund et al 2019 ; Nugroho and Frank 2011 ), molecular and behavioral biomarkers (Bielen et al 2016 ; Falfushynska et al 2013 ; Hartmann et al 2016 ; Oliviera et al 2015 ), and mortality (Kováts et al 2010 ; Oliviera et al 2015 ). The reproductive cycle of A. anatina includes a gravid stage during autumn/winter (Aldridge 1999 ; Hinzmann et al 2013 ), potentially increasing variability in transcriptional and biochemical biomarkers (Ekelund Ugge et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

Ugge

Jonsson

Berglund

2021

Environ Sci Pollut Res

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Using a selection of molecular biomarkers, we evaluated responses in freshwater mussels (Anodonta anatina) exposed to effluent from an industrial wastewater treatment facility. The aims of this work were to (1) assess biomarkers of general toxicity under sublethal exposure to an anthropogenic mixture of chemicals, represented by an arbitrary effluent, and (2) evaluate the potential of A. anatina as a bioindicator of pollution. Adult mussels (n = in total 32; 24 males and 8 females) were exposed (96 h) in the laboratory to a fixed dilution of effluent or to a control treatment of standardized freshwater. Metal concentrations were in general higher in the effluent, by an order of magnitude or more, compared to the control. Toxic unit estimates were used as proxies of chemical stress, and Cu, Ni, and Zn were identified as potential major contributors (Cu> Ni > Zn). Six transcriptional (cat, gst, hsp70, hsp90, mt, sod) and two biochemical (AChE, GST) biomarkers were analyzed in two tissues, gills, and digestive glands. Out of the 16 responses (eight biomarkers × two tissues), 14 effect sizes were small (within ± 28 % of control) and differences non-significant (p > 0.05). Results did however show that (1) AChE activity increased by 40% in gills of exposed mussels compared to control, (2) hsp90 expression was 100% higher in exposed female gills compared to control, and (3) three marker signals (AChE in both tissues, and hsp70 in gills) differed between sexes, independent of treatment. Results highlight a need for further investigation of molecular biomarker variability and robustness in A. anatina.

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“…On the seventh day of the experiment, gills contained elevated amounts of total lipids, chiefly membrane fractions (sterols and PLs), which was likely due to intensive formation of vesicles (metal-containing granules and autophagosomes) participating in compartmentalisation of the metals and their detoxification in the mussels [ 33 , 34 ]. The incomplete excretion of copper from mussels reportedly takes 10 days [ 40 ]. Active synthesis of membrane lipids apparently facilitates copper detoxification in mussels.…”

Section: Resultsmentioning

confidence: 99%

Copper and Nickel Induce Changes in the Lipid and Fatty Acid Composition of Anodonta cygnea

Fokina

2023

JoX

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The effect of copper and nickel ions on the lipid composition of freshwater mussels Anodonta cygnea was investigated using an aquarium experiment. The contents of the main lipid classes were determined using thin layer chromatography and spectrophotometry, and the fatty acid composition was analysed using gas–liquid chromatography. The results indicated that copper and nickel had different effects on the mussels’ lipid composition, with copper producing less effect on the composition of lipids and fatty acids than nickel. On the first experiment day, excessive copper content in the organism caused oxidative stress and modifications in membrane lipids, which returned to their initial level by the end of the experiment. Nickel accumulated predominantly in gills; however, significant modifications in lipids and fatty acids were seen also in the digestive gland from the first day of the experiment. This indicated the activation of nickel-induced lipid peroxidation processes. Moreover, this study revealed a dose-dependent effect of nickel on lipid composition, which was likely related to the development of compensatory biochemical mechanisms in response to nickel-induced oxidative stress. A comparative study of the lipid composition alteration in mussels in response to copper and nickel action revealed the consequences of the toxic impact of metal ions and the defensive mechanisms that organisms employ to detoxify and remove xenobiotics.

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Uptake, distribution, and bioaccumulation of copper in the freshwater musselAnodonta anatina

Cited by 11 publications

References 19 publications

Transcriptional and biochemical biomarker responses in a freshwater mussel (Anodonta anatina) under environmentally relevant Cu exposure

Transcriptional and biochemical biomarker responses in a freshwater mussel (Anodonta anatina) under environmentally relevant Cu exposure

Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

Copper and Nickel Induce Changes in the Lipid and Fatty Acid Composition of Anodonta cygnea

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