Hydroxypropylmethyl cellulose (HPMC) crosslinked keratin/hydroxyapatite (HA) scaffold fabrication, characterization and in vitro biocompatibility assessment as a bone graft for alveolar bone regeneration

Feroz, Sandleen; Dias, George J.

doi:10.1016/j.heliyon.2021.e08294

Cited by 42 publications

(33 citation statements)

References 60 publications

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“…The mechanical properties of the 3-dimensional layered scaffold are mainly governed by the inherent compressive modulus of the organic component, the presence of a crosslinker, and interfacial interaction of the organic and inorganic phases. Previous studies have suggested that the biomechanics of scaffolds is an important factor from a clinical perspective 14,53,54 . These scaffolds should provide stable xation for e cient regeneration of new bony tissues.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

“…These aligned with the previous studies that have reported decreased cellular proliferation due to the osteogenic differentiation of cells by hydroxyapatite. 13,34,54,64 . A recent study conducted by our group also observed an increased cell number in keratin compared to HPMC crosslinked keratin/HA scaffolds at 48 and 72 h 2 .…”

Section: Cell Proliferationmentioning

confidence: 99%

“…The topographic features of the surface of the scaffold play a crucial role in cell attachment and the observed results further signify the fact that the cells showed strong a nity to the surface of both keratin and keratin/HA scaffolds. The appearance of this sheet-like morphology involving multiple surrounding cells is the initiation of the formation of a multilayer structure 54 .…”

Section: Evaluation Of Cell Adhesion/morphologymentioning

confidence: 99%

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WITHDRAWN: Biomimetic dual layered keratin/ hydroxyapatite scaffolds for alveolar bone regeneration

Feroz

Muhammad

Cathro

et al. 2022

Preprint

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A new biomimetic dual layered keratin/hydroxyapatite scaffold was designed using the iterative freeze-drying technique. The physical, structural, and chemical properties of the keratin/hydroxyapatite (HA) scaffolds were studied using several analytical techniques. The developed multilayered, interconnected, porous keratin scaffold with different HA content in the outer and inner layer, mimics the inherent gradient structure of alveolar bone. The FT-IR analysis confirmed that the secondary structure of keratin was not destroyed with the successful incorporation of hydroxyapatite particles during the process of scaffold fabrication. X-ray diffraction analysis showed that the keratin and hydroxyapatite retained their crystalline structures in the prepared keratin/HA scaffolds. SEM studies showed an interconnected porous architecture of the prepared scaffolds with seamless integration between the upper and lower layers. EDX analysis showed that the inorganic phases of both the upper and lower layers of the keratin/HA scaffold mainly consist of calcium and phosphorous ions. EDX mapping confirmed the even distribution of calcium (Ca) and phosphorous (P) in both the upper and lower layers of the scaffold. The prepared scaffolds showed a gradual degradation during the 28-day incubation period in PBS. The incorporation of HA improved the mechanical properties of keratin/HA scaffolds. The keratin/HA scaffolds exhibited superior mechanical properties in terms of Young’s modulus and compressive strength in comparison to pure keratin scaffolds. The biocompatibility studies suggested that both keratin and keratin/HA scaffolds were cytocompatible, in terms of cell viability and proliferation. Furthermore, it showed that both the tested materials can be served as an ideal substrate for the differentiation of Saos-2 cells, leading to mineralization of the extracellular matrix. In summary, the development of a non-toxic green method was investigated for keratin extraction to fabricate keratin/HA scaffolds and our results showed great potential for the use of these scaffolds to regenerate alveolar bone due to their structural similarity and excellent in vitro biocompatibility.

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Section: Mechanical Propertiesmentioning

confidence: 99%

Section: Cell Proliferationmentioning

confidence: 99%

Section: Evaluation Of Cell Adhesion/morphologymentioning

confidence: 99%

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WITHDRAWN: Biomimetic dual layered keratin/ hydroxyapatite scaffolds for alveolar bone regeneration

Feroz

Muhammad

Cathro

et al. 2022

Preprint

Self Cite

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“…Other stretching vibration bands related to C-H and C-O were observed at 2926 cm −1 and 1058 cm −1 , respectively. The characteristic vibration peaks associated with HPMC appeared at 1455 cm −1 related to the methoxy (-OCH 3 ) group and at 946 cm −1 , corresponding to the pyranose ring [ 66 ].…”

Section: Resultsmentioning

confidence: 99%

Design, Characterization, and Anticancer and Antimicrobial Activities of Mucoadhesive Oral Patches Loaded with Usnea barbata (L.) F. H. Wigg Ethanol Extract F-UBE-HPMC

et al. 2022

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The oral cavity’s common pathologies are tooth decay, periodontal disease, and oral cancer; oral squamous cell carcinoma (OSCC) is the most frequent oral malignancy, with a high mortality rate. Our study aims to formulate, develop, characterize, and pharmacologically investigate the oral mucoadhesive patches (F-UBE-HPMC) loaded with Usnea barbata (L.) F.H. Wigg dry ethanol extract (UBE), using HPMC K100 as a film-forming polymer. Each patch contains 312 µg UBE, with a total phenolic content (TPC) of 178.849 µg and 33.924 µg usnic acid. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were performed for their morphological characterization, followed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). Pharmacotechnical evaluation involved the measurement of the specific parameters for mucoadhesive oral patches as follows: weight uniformity, thickness, folding endurance, tensile strength, elongation, moisture content, pH, disintegration time, swelling rate, and ex vivo mucoadhesion time. Thus, each F-UBE-HPMC has 104 ± 4.31 mg, a pH = 7.05 ± 0.04, a disintegration time of 130 ± 4.14 s, a swelling ratio of 272 ± 6.31% after 6 h, and a mucoadhesion time of 102 ± 3.22 min. Then, F-UBE-HPMCs pharmacological effects were investigated using brine shrimp lethality assay (BSL assay) as a cytotoxicity prescreening test, followed by complex flow cytometry analyses on blood cell cultures and oral epithelial squamous cell carcinoma CLS-354 cell line. The results revealed significant anticancer effects by considerably increasing oxidative stress and blocking DNA synthesis in CLS-354 cancer cells. The antimicrobial potential against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27353, Candida albicans ATCC 10231, and Candida parapsilosis ATCC 22019 was assessed by a Resazurin-based 96-well plate microdilution method. The patches moderately inhibited both bacteria strains growing and displayed a significant antifungal effect, higher on C. albicans than on C. parapsilosis. All these properties lead to considering F-UBE-HPMC suitable for oral disease prevention and therapy.

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“…Brahimi (2022) et al prepared highly porous chitosan/HA scaffolds through freeze gelation by varying the HA content [ 88 ]. Feroz (2021) et al developed a novel hydroxypropylmethyl cellulose (HPMC) crosslinked keratin scaffold with HA as the main inorganic component for alveolar bone regeneration by freeze-drying technology [ 89 ]. By incorporating HANPs into the polymer matrix of dextran/chitosan, El-Meliegy (2018) et al realized a novel composite scaffold by freeze-drying technique and determined the effect of HANPs on scaffold morphology and mechanical properties [ 90 ].…”

Section: Recent Strategies For Compounding Natural and Synthetic Poly...mentioning

confidence: 99%

Advances of Hydroxyapatite Hybrid Organic Composite Used as Drug or Protein Carriers for Biomedical Applications: A Review

et al. 2022

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Hydroxyapatite (HA), especially in the form of HA nanoparticles (HANPs), has excellent bioactivity, biodegradability, and osteoconductivity and therefore has been widely used as a template or additives for drug delivery in clinical applications, such as dentistry and orthopedic repair. Due to the atomically anisotropic distribution on the preferred growth of HA crystals, especially the nanoscale rod-/whisker-like morphology, HA can generally be a good candidate for carrying a variety of substances. HA is biocompatible and suitable for medical applications, but most drugs carried by HANPs have an initial burst release. In the adsorption mechanism of HA as a carrier, specific surface area, pore size, and porosity are important factors that mainly affect the adsorption and release amounts. At present, many studies have developed HA as a drug carrier with targeted effect, porous structure, and high porosity. This review mainly discusses the influence of HA structures as a carrier on the adsorption and release of active molecules. It then focuses on the benefits and effects of different types of polymer-HA composites to re-examine the proteins/drugs carry and release behavior and related potential clinical applications. This literature survey can be divided into three main parts: 1. interaction and adsorption mechanism of HA and drugs; 2. advantages and application fields of HA/organic composites; 3. loading and drug release behavior of multifunctional HA composites in different environments. This work also presents the latest development and future prospects of HA as a drug carrier.

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Hydroxypropylmethyl cellulose (HPMC) crosslinked keratin/hydroxyapatite (HA) scaffold fabrication, characterization and in vitro biocompatibility assessment as a bone graft for alveolar bone regeneration

Cited by 42 publications

References 60 publications

WITHDRAWN: Biomimetic dual layered keratin/ hydroxyapatite scaffolds for alveolar bone regeneration

WITHDRAWN: Biomimetic dual layered keratin/ hydroxyapatite scaffolds for alveolar bone regeneration

Design, Characterization, and Anticancer and Antimicrobial Activities of Mucoadhesive Oral Patches Loaded with Usnea barbata (L.) F. H. Wigg Ethanol Extract F-UBE-HPMC

Advances of Hydroxyapatite Hybrid Organic Composite Used as Drug or Protein Carriers for Biomedical Applications: A Review

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