Hydroxyapatite nucleation and growth on collagen electrospun fibers controlled with different mineralization conditions and phosvitin

Jie, Yilin; Cai, Zhaoxia; Li, Shanshan; Xie, Zhuqing; Ma, Meihu; Huang, Xi

doi:10.1007/s13233-017-5091-z

Cited by 17 publications

(8 citation statements)

References 66 publications

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“…Besides the narrow diameter distributions, nanofibrous scaffolds possess large surface area for cell adhesion and interconnected pore structure, which provides nutrient supply to the cells [12]. There are various methods to fabricate nanofibrous scaffolds for bone regeneration applications; such as selfassembly [13], phase separation [8], template synthesis [14], and electrospinning [15,16]. Among them, electrospinning is regarded as a simple, economical and versatile method capable of providing continuous ultrafine fibers, which can replicate the nanoscale properties of the natural extracellular matrix (ECM) [17,18].…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Hokmabad

Davaran

Aghazadeh

et al. 2018

Tissue Eng Regen Med

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BACKGROUND: The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(e-caprolactone)poly(ethylene glycol)-poly(e-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica (n-SiO 2) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering. METHODS: We evaluated the effect of n-HA and n-SiO 2 incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay. RESULTS: All the samples demonstrated bead-less morphologies with an average diameter in the range of 190-260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with n-SiO 2. While the hydrophilicity of n-SiO 2 incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to n-SiO 2 incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and n-SiO 2. CONCLUSION: Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Hokmabad

Davaran

Aghazadeh

et al. 2018

Tissue Eng Regen Med

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“…Inclusion was determined by relevance to the topic (i.e., must examine collagen-containing electrospun materials for tissue engineering/regenerative medicine applications) ( Figure 1 ). After assessment [ 16 , 17 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ,…”

Section: Methodsmentioning

confidence: 99%

Collagen-Based Electrospun Materials for Tissue Engineering: A Systematic Review

Blackstone

Powell

2021

Bioengineering

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Collagen is a key component of the extracellular matrix (ECM) in organs and tissues throughout the body and is used for many tissue engineering applications. Electrospinning of collagen can produce scaffolds in a wide variety of shapes, fiber diameters and porosities to match that of the native ECM. This systematic review aims to pool data from available manuscripts on electrospun collagen and tissue engineering to provide insight into the connection between source material, solvent, crosslinking method and functional outcomes. D-banding was most often observed in electrospun collagen formed using collagen type I isolated from calfskin, often isolated within the laboratory, with short solution solubilization times. All physical and chemical methods of crosslinking utilized imparted resistance to degradation and increased strength. Cytotoxicity was observed at high concentrations of crosslinking agents and when abbreviated rinsing protocols were utilized. Collagen and collagen-based scaffolds were capable of forming engineered tissues in vitro and in vivo with high similarity to the native structures.

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“…The carboxylic groups (-COO − ) of glutamic (Glu), aspartic (Asp), and glucuronic (Glc) acid sidechains attract and bind two Ca 2+ ions from solution and further attract PO 4 3− ions so as to form a connective CaP network with consequential nucleation [38,39]. A large number of NCPs and phosphorylated modifications thereof [40,41] are strongly involved in the matrix vesicle-mediated mineralisation process and play essential roles in the regulation of bone biomineralisation, such as via the initiation of the formation of ACP, apatite nucleation, and crystal growth, as well as via inhibition [42]. It is generally assumed that NCPs regulate solution crystal growth via some sort of 'epitaxial' relationship between specific crystallographic faces and specific protein conformers [43].…”

Section: Biomineralisationmentioning

confidence: 99%

“…(1) The direct, in-situ mineralisation of COL(GEL) [56,[58][59][60][61][62][63][64][65][66][67][68], (2) The biomimetic deposition of CaPs on pre-arranged COL(GEL) scaffolds [41,[69][70][71][72][73] from Ca 2+ and PO 4 3− ion-containing solutions, e.g., simulated body fluids.…”

Section: Collagen and Gelatin/cap Systemsmentioning

confidence: 99%

The Significance and Utilisation of Biomimetic and Bioinspired Strategies in the Field of Biomedical Material Engineering: The Case of Calcium Phosphat—Protein Template Constructs

Šupová

2020

Materials

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This review provides a summary of recent research on biomimetic and bioinspired strategies applied in the field of biomedical material engineering and focusing particularly on calcium phosphate—protein template constructs inspired by biomineralisation. A description of and discussion on the biomineralisation process is followed by a general summary of the application of the biomimetic and bioinspired strategies in the fields of biomedical material engineering and regenerative medicine. Particular attention is devoted to the description of individual peptides and proteins that serve as templates for the biomimetic mineralisation of calcium phosphate. Moreover, the review also presents a description of smart devices including delivery systems and constructs with specific functions. The paper concludes with a summary of and discussion on potential future developments in this field.

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Hydroxyapatite nucleation and growth on collagen electrospun fibers controlled with different mineralization conditions and phosvitin

Cited by 17 publications

References 66 publications

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Collagen-Based Electrospun Materials for Tissue Engineering: A Systematic Review

The Significance and Utilisation of Biomimetic and Bioinspired Strategies in the Field of Biomedical Material Engineering: The Case of Calcium Phosphat—Protein Template Constructs

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