Hydroxyapatite nanoparticles in situ grown on carbon nanotube as a reinforcement for poly (ε-caprolactone) bone scaffold

Feng, Pei; Wang, Kai; Shuai, Yang; Peng, Shuping; Hu, Yongbin; Shuai, Cijun

doi:10.1016/j.mtadv.2022.100272

Cited by 33 publications

(22 citation statements)

References 77 publications

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“…These results con rmed that heat treatment at 1,000°C completely transformed the DB into HA. The atomic ratio of Ca to P in the HA was 1.63 (Table S2), which is similar to that (1.60-1.70) in the human skeleton [27]. Hence, the HA obtained in our experiments has a suitable composition for bones.…”

Section: Resultssupporting

confidence: 80%

Fabrication and functionality of 3D-printed porous scaffolds composing natural derivatives of duck bones and fish shells and poly(ε-caprolactone)

Shih

Wang

et al. 2022

Preprint

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Recycled duck bones (DBs) and fish shells were processed into natural derivatives. Through innovative design, these natural derivatives were then combined with biopolymers to create a new type of ecofriendly filament suitable for three-dimensional (3D) printing of scaffolds for bone regeneration. The DBs and fish shells were thermally processed to produce DB-derived hydroxyapatite (HA) and fish shell-derived Ca(OH)2 (TAS), respectively. Poly(ε-caprolactone) (PCL), HA, and TAS were combined and fabricated into new composite filaments, which were then transformed into scaffolds using 3D printing technology. The structure and antibacterial behaviors of the obtained composite scaffolds were studied. Alone, PCL showed no bacterial inhibition. MHA (a mix of HA and TAS) was added to PCL to form a PCL/MHA composite material, which significantly improved the functional properties of PCL and enhanced cell attachment and proliferation. The Ca(OH)2 content of TAS was responsible for the antibacterial effect. The PCL/MHA composites were porous and displayed enhanced osteoblast proliferation in vitro. The osteoblast cell population do not affected when cultured on the PCL/HA and PCL/MHA series composites according to cell cycle distribution analysis. The surfaces of the various PCL/HA and PCL/MHA composites showed elevated levels of calcium and phosphorus compounds when exposed to simulated body fluids. Calcium and phosphate ions were rapidly deposited on PCL/HA and PCL/MHA composite scaffolds in osteoblasts according to the cell mineralization assay. Our findings suggest great potential of the PCL/HA and PCL/MHA composite scaffolds in bone tissue engineering applications.

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Section: Resultssupporting

confidence: 80%

Fabrication and functionality of 3D-printed porous scaffolds composing natural derivatives of duck bones and fish shells and poly(ε-caprolactone)

Shih

Wang

et al. 2022

Preprint

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“… 16 At the same time, the incorporation of nanoparticles could slightly enhance the mechanical characteristics and stability. 17 Previous research in the area of bone regeneration has shown that a hydrogel scaffold made primarily of collagen and hyaluronic acid provides an environment that closely resembles the extracellular matrix of cartilage and could be utilized to repair cartilage defects. 18 Hydrogels are extensively used in the medical field, while their application in tissue engineering is limited by their poor mechanical strength.…”

Section: Introductionmentioning

confidence: 99%

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Li¹,

Li²,

Song³

et al. 2022

IJN

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Background: Clinicians frequently face difficulties when trying to fix bone abnormalities. Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure. Meanwhile, cerium oxide nanoparticles (nCeO 2 ) demonstrated high antioxidant enzyme simulation activity. Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO 2 hydrogel scaffold was developed and evaluated. Methods: GA-nCeO 2 hydrogel scaffold was prepared by the lyophilized method and characterized. The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope. CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation. Then the osteogenic differentiation in early and late stages was discussed. The expression of osteogenic genes was also detected by RT-PCR. Finally, a bone defect model was made in SD rats, and bone formation in vivo was detected. Results:The results showed that GA-nCeO 2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%. The GA-nCeO 2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO 2 . Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO 2 hydrogel. The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity. Conclusion: Due to its favorable safety, degradability, and bone formation property, GA-nCeO 2 hydrogel was anticipated to be used as a potential bone defect healing material.

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“… 16 , 18 Moreover, Dang et al also demonstrated the synergistic effects of Mg and PTH in promoting bone regeneration. 18 Polycaprolactone (PCL) has been widely used as the scaffold in bone regeneration engineering, 19 , 20 therefore, we applied PCL in the nanofiber as the main scaffold.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Pro-Osteogenic Coaxially Electrospun Magnesium Oxide Nanoparticles-Polycaprolactone /Parathyroid Hormone-Polycaprolactone Composite Barrier Membrane for Guided Bone Regeneration

Dong¹,

Yao²,

Cai³

et al. 2023

IJN

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Introduction An antibacterial and pro-osteogenic coaxially electrospun nanofiber guided bone regeneration (GBR) membrane was fabricated to satisfy the complicated and phased requirements of GBR process. Methods In this study, we synthesize dual-functional coaxially electrospun nanofiber GBR membranes by encapsulating parathyroid hormone (PTH) in the core layer and magnesium oxide nanoparticles (MgONPs) in the shell layer (MgONPs-PCL/PTH-PCL). Herein, the physicochemical characterization of MgONPs-PCL/PTH-PCL, the release rates of MgONPs and PTH, and antibacterial efficiency of the new membrane were evaluated. Furthermore, the pro-osteogenicity of the membranes was assessed both in-vitro and in-vivo. Results We successfully fabricated a coaxially electrospun nanofiber MgONPs-PCL/PTH-PCL membrane with the majority of nanofibers (>65%) ranged from 0.40~0.60μm in diameter. MgONPs-PCL/PTH-PCL showed outstanding antibacterial potential against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) through the release of MgONPs. We also discovered that the incorporation of MgONPs significantly prolonged the release of PTH. Furthermore, both the in-vivo and in-vitro studies demonstrated that high dosage of PTH promoted pro-osteogenicity of the membrane to improve bone regeneration efficacy with the presence of MgONPs. Conclusion The new composite membrane is a promising approach to enhance bone regeneration in periodontitis or peri-implantitis patients with large-volume bone defects.

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Hydroxyapatite nanoparticles in situ grown on carbon nanotube as a reinforcement for poly (ε-caprolactone) bone scaffold

Cited by 33 publications

References 77 publications

Fabrication and functionality of 3D-printed porous scaffolds composing natural derivatives of duck bones and fish shells and poly(ε-caprolactone)

Fabrication and functionality of 3D-printed porous scaffolds composing natural derivatives of duck bones and fish shells and poly(ε-caprolactone)

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Antimicrobial and Pro-Osteogenic Coaxially Electrospun Magnesium Oxide Nanoparticles-Polycaprolactone /Parathyroid Hormone-Polycaprolactone Composite Barrier Membrane for Guided Bone Regeneration

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