Antimicrobial and Pro-Osteogenic Coaxially Electrospun Magnesium Oxide Nanoparticles-Polycaprolactone /Parathyroid Hormone-Polycaprolactone Composite Barrier Membrane for Guided Bone Regeneration

Abstract

Introduction An antibacterial and pro-osteogenic coaxially electrospun nanofiber guided bone regeneration (GBR) membrane was fabricated to satisfy the complicated and phased requirements of GBR process. Methods In this study, we synthesize dual-functional coaxially electrospun nanofiber GBR membranes by encapsulating parathyroid hormone (PTH) in the core layer and magnesium oxide nanoparticles (MgONPs) in the shell layer (MgONPs-PCL/PTH-PCL). Herein, the physicochemical characterization of MgONPs-PCL/PTH-PCL, the release rates of MgONPs and PTH, and antibacterial efficiency of the new membrane were evaluated. Furthermore, the pro-osteogenicity of the membranes was assessed both in-vitro and in-vivo. Results We successfully fabricated a coaxially electrospun nanofiber MgONPs-PCL/PTH-PCL membrane with the majority of nanofibers (>65%) ranged from 0.40~0.60μm in diameter. MgONPs-PCL/PTH-PCL showed outstanding antibacterial potential against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) through the release of MgONPs. We also discovered that the incorporation of MgONPs significantly prolonged the release of PTH. Furthermore, both the in-vivo and in-vitro studies demonstrated that high dosage of PTH promoted pro-osteogenicity of the membrane to improve bone regeneration efficacy with the presence of MgONPs. Conclusion The new composite membrane is a promising approach to enhance bone regeneration in periodontitis or peri-implantitis patients with large-volume bone defects.

Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts

“…Coaxial electrospinning is a novel technique for producing core-shell nanofibers that provide a robust structure and deliver bioactive agents ( Yin et al, 2017 ; Liu et al, 2020 ). It has been applied to various fields, such as antibacterial materials ( Dong et al, 2023 ), tissue engineering scaffolds ( Iliou et al, 2022 ; Nagiah et al, 2022 ), catalysts ( Choi et al, 2016 ), and others. Especially, this technique has unique advantages for creating anticoagulant materials ( Huang et al, 2013 ; Kuang et al, 2018 ; Strobel et al, 2018 ).…”

Addressing Antimicrobial Properties in Guided Tissue/Bone Regeneration Membrane: Enhancing Effectiveness in Periodontitis Treatment

“…This, in effect, prevents the failure of guided bone regeneration due to early exposure to oral pathogenic bacteria. 131 Furthermore, Xuezhe Liu and the team have made significant strides by incorporating MgO-NPs into biodegradable PLA/gelatin periodontal membranes, enhancing their antibacterial properties and osteogenic capabilities. Their findings underscore the improved overall characteristics of these membranes.…”

“…This gradual release, in turn, showcases its remarkable potential to effectively eradicate any lingering bacteria within periodontal tissues. This, in effect, prevents the failure of guided bone regeneration due to early exposure to oral pathogenic bacteria . Furthermore, Xuezhe Liu and the team have made significant strides by incorporating MgO-NPs into biodegradable PLA/gelatin periodontal membranes, enhancing their antibacterial properties and osteogenic capabilities.…”

Recent Advances in Functionalized Electrospun Membranes for Periodontal Regeneration

“…can co-ordinate hard and soft tissue responses during the defect healing process to ensure multi-tissue regeneration [ 81 ], ( Figure 6 ). Sequential spinning or multi-layering may be employed to fabricate multilayered membranes, or alternatively, ES can be combined with other technologies like tri-dimensional printing scaffolds [ 156 ], freeze-drying or phase separation processes [ 91 ].…”