Hydrogenase Activity Control at Desulfovibrio vulgaris Cell-Coated Carbon Electrodes: Biochemical and Chemical Factors Influencing the Mediated Bioelectrocatalysis

Lojou, Élisabeth; Durand, Marie-Claire; Dolla, A.; Bianco, Pierre

doi:10.1002/1521-4109(200207)14:13<913::aid-elan913>3.0.co;2-n

Cited by 62 publications

(35 citation statements)

References 17 publications

(23 reference statements)

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“…H 2 oxidation was not realized by enzymes themselves but by whole cells of the sulfate reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH), which is known to contain several Hases from different classes. [292][293] In the presence of suitable redox mediators (methyl viologen, MeV, for Hase and ABTS for BOD), the cell was demonstrated to deliver a current of 0.9 mA at 1.0 V. 30 years of mechanistic studies to understand the enzymatic reactions driven by Hase on an electrode, then the identification of O 2 -tolerant Hases, [90] CHEMELECTROCHEM REVIEWS www.chemelectrochem.org were necessary to design the first H 2 /O 2 EBFC based on enzymes both at the anode and the cathode. The device was first reported in 2002 by Tarasevich et al, and delivered a power density of more than 300 mW/cm 2 , but no details on the source of enzymes, nor on the device were given.…”

Section: H 2 /O 2 Biofuel Cells: a Short But Attractive Storymentioning

confidence: 97%

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

et al. 2014

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Among sustainable alternatives to fossil fuel, proton exchange membrane fuel cells are promising devices that deliver electricity from hydrogen and oxygen, producing only water. The transformation of the fuel and oxidant however relies on rare‐metal catalysts. H2/O2 enzymatic biofuel cells thus emerge as sustainable biotechnological devices in which chemical catalysts are replaced by hydrogenases at the anode and multicopper oxidases at the cathode. This review discusses the recent breakthroughs and the limitations in all the components of H2/O2 biofuel cells: 1) Catalytic mechanisms involved in multicopper oxidases and hydrogenases, in addition to the new potential of enzymes from the biodiversity pool, which exhibit outstanding properties. 2) The molecular basis for oriented enzyme immobilization on the electrochemical interfaces as a prerequisite for a fast direct electron‐transfer rate. 3) The requirement for 3D networks to enhance current densities. 4) The production of H2 from biomass. 5) The history of H2/O2 biofuel cells and recent devices, which also highlights the remaining issues that will allow the use of such devices in low‐power applications.

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Section: H 2 /O 2 Biofuel Cells: a Short But Attractive Storymentioning

confidence: 97%

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

et al. 2014

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“…Electrochemical techniques typically used to study purified redox proteins (3,4,7) and cell extracts (26,45) could likely be adapted for the study of metal-reducing bacteria. While protein voltammetry is limited by the success of purification and immobilization of enzymes (17,49), metal-reducing bacteria represent a self-assembling multienzyme system that naturally interfaces with electrodes.…”

mentioning

confidence: 99%

“…incorporated into, electrode materials (26,45,46,61). In addition, researchers have washed and concentrated whole cells for brief voltammetry analyses and detected possible redoxactive agents on the external surfaces of cells (39).…”

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confidence: 99%

Microbial Biofilm Voltammetry: Direct Electrochemical Characterization of Catalytic Electrode-Attached Biofilms

Marsili

Rollefson

Baron

et al. 2008

Appl Environ Microbiol

452

361

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While electrochemical characterization of enzymes immobilized on electrodes has become common, there is still a need for reliable quantitative methods for study of electron transfer between living cells and conductive surfaces. This work describes growth of thin (<20 m) Geobacter sulfurreducens biofilms on polished glassy carbon electrodes, using stirred three-electrode anaerobic bioreactors controlled by potentiostats and nondestructive voltammetry techniques for characterization of viable biofilms. Routine in vivo analysis of electron transfer between bacterial cells and electrodes was performed, providing insight into the main redox-active species participating in electron transfer to electrodes. At low scan rates, cyclic voltammetry revealed catalytic electron transfer between cells and the electrode, similar to what has been observed for pure enzymes attached to electrodes under continuous turnover conditions. Differential pulse voltammetry and electrochemical impedance spectroscopy also revealed features that were consistent with electron transfer being mediated by an adsorbed catalyst. Multiple redox-active species were detected, revealing complexity at the outer surfaces of this bacterium. These techniques provide the basis for cataloging quantifiable, defined electron transfer phenotypes as a function of potential, electrode material, growth phase, and culture conditions and provide a framework for comparisons with other species or communities.

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“…In addition to the above-cited application of G. sulfurreducens for hydrogen production in microbial electrolysis cells, microbial biocathode concepts that were developed so far for catalyzing cathodic hydrogen production were based on an immobilized pure culture of Desulfovibrio vulgaris [44] with methyl viologen as a redox mediator. A microbial biocathode for hydrogen production was described by Rozendal: this has been shown to operate for up to 2000 h and it was based on a selected mixed culture of electrochemically active micro-organisms, forming biofilm layers on the surface of graphite felt electrodes [45].…”

Section: Electrons Exchangementioning

confidence: 99%

“…This is in agreement with the observation that different cellular fraction showed electrochemical response on a carbon electrode when hydrogen was either oxidized or formed. In particular hydrogen oxidation was found to occur mainly when the periplasmic fraction was immobilized while hydrogen formation was equally occurring when either the periplasmic or the membrane fraction were immobilized [44]. Furthermore, a network of c-type cytochromes provides the electrical wiring between the different enzymes and they represent the perfect targets for integrating the extracellular electron transfer network with the hydrogen metabolism.…”

Section: Materials For Pmecmentioning

confidence: 99%

Development of a Photosynthetic Microbial Electrochemical Cell (PMEC) Reactor Coupled with Dark Fermentation of Organic Wastes: Medium Term Perspectives

2015

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Abstract:In this article the concept, the materials and the exploitation potential of a photosynthetic microbial electrochemical cell for the production of hydrogen driven by solar power are investigated. In a photosynthetic microbial electrochemical cell, which is based on photosynthetic microorganisms confined to an anode and heterotrophic bacteria confined to a cathode, water is split by bacteria hosted in the anode bioactive film. The generated electrons are conveyed through external "bio-appendages" developed by the bacteria to transparent nano-pillars made of indium tin oxide (ITO), Fluorine-doped tin oxide (FTO) or other conducting materials, and then transferred to the cathode. On the other hand, the generated protons diffuse to the cathode via a polymer electrolyte membrane, where they are reduced by the electrons by heterotrophic bacteria growing attached to a similar pillared structure as that envisaged for the anode and supplemented with a specific low cost substrate (e.g., organic waste, anaerobic digestion outlet). The generated oxygen is released to the atmosphere or stored, while the produced pure hydrogen leaves the electrode through the porous layers. In addition, the integration of the photosynthetic microbial electrochemical cell system with dark fermentation as acidogenic step of anaerobic digester, which is able to produce additional H2, and the use of microbial fuel cell, feed with the residues of dark fermentation (mainly volatile fatty acids), to produce the necessary extra-bias for the photosynthetic microbial electrochemical cell is here analyzed to reveal the potential benefits to this novel integrated technology. OPEN ACCESS

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Hydrogenase Activity Control at Desulfovibrio vulgaris Cell-Coated Carbon Electrodes: Biochemical and Chemical Factors Influencing the Mediated Bioelectrocatalysis

Cited by 62 publications

References 17 publications

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Microbial Biofilm Voltammetry: Direct Electrochemical Characterization of Catalytic Electrode-Attached Biofilms

Development of a Photosynthetic Microbial Electrochemical Cell (PMEC) Reactor Coupled with Dark Fermentation of Organic Wastes: Medium Term Perspectives

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Hydrogenase Activity Control at Desulfovibrio vulgaris Cell-Coated Carbon Electrodes: Biochemical and Chemical Factors Influencing the Mediated Bioelectrocatalysis

Cited by 62 publications

References 17 publications

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

Microbial Biofilm Voltammetry: Direct Electrochemical Characterization of Catalytic Electrode-Attached Biofilms

Development of a Photosynthetic Microbial Electrochemical Cell (PMEC) Reactor Coupled with Dark Fermentation of Organic Wastes: Medium Term Perspectives

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Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective