Bacteria able to transfer electrons to metals are key agents in biogeochemical metal cycling, subsurface bioremediation, and corrosion processes. More recently, these bacteria have gained attention as the transfer of electrons from the cell surface to conductive materials can be used in multiple applications. In this work, we adapted electrochemical techniques to probe intact biofilms of Shewanella oneidensis MR-1 and Shewanella sp. MR-4 grown by using a poised electrode as an electron acceptor. This approach detected redox-active molecules within biofilms, which were involved in electron transfer to the electrode. A combination of methods identified a mixture of riboflavin and riboflavin-5 -phosphate in supernatants from biofilm reactors, with riboflavin representing the dominant component during sustained incubations (>72 h). Removal of riboflavin from biofilms reduced the rate of electron transfer to electrodes by >70%, consistent with a role as a soluble redox shuttle carrying electrons from the cell surface to external acceptors. Differential pulse voltammetry and cyclic voltammetry revealed a layer of flavins adsorbed to electrodes, even after soluble components were removed, especially in older biofilms. Riboflavin adsorbed quickly to other surfaces of geochemical interest, such as Fe(III) and Mn(IV) oxy(hydr)oxides. This in situ demonstration of flavin production, and sequestration at surfaces, requires the paradigm of soluble redox shuttles in geochemistry to be adjusted to include binding and modification of surfaces. Moreover, the known ability of isoalloxazine rings to act as metal chelators, along with their electron shuttling capacity, suggests that extracellular respiration of minerals by Shewanella is more complex than originally conceived.bioelectrochemistry ͉ biogeochemistry ͉ redox mediator ͉ riboflavin
While electrochemical characterization of enzymes immobilized on electrodes has become common, there is still a need for reliable quantitative methods for study of electron transfer between living cells and conductive surfaces. This work describes growth of thin (<20 m) Geobacter sulfurreducens biofilms on polished glassy carbon electrodes, using stirred three-electrode anaerobic bioreactors controlled by potentiostats and nondestructive voltammetry techniques for characterization of viable biofilms. Routine in vivo analysis of electron transfer between bacterial cells and electrodes was performed, providing insight into the main redox-active species participating in electron transfer to electrodes. At low scan rates, cyclic voltammetry revealed catalytic electron transfer between cells and the electrode, similar to what has been observed for pure enzymes attached to electrodes under continuous turnover conditions. Differential pulse voltammetry and electrochemical impedance spectroscopy also revealed features that were consistent with electron transfer being mediated by an adsorbed catalyst. Multiple redox-active species were detected, revealing complexity at the outer surfaces of this bacterium. These techniques provide the basis for cataloging quantifiable, defined electron transfer phenotypes as a function of potential, electrode material, growth phase, and culture conditions and provide a framework for comparisons with other species or communities.
The ability of Geobacter sulfurreducens to utilize electrodes as electron acceptors provides a system for monitoring mechanisms of electron transfer beyond the cell surface. This study examined the physiology of extracellular electron transfer during many stages of growth, and in response to short-and long-term changes in electron acceptor potential. When G. sulfurreducens was grown on planar potentiostat-controlled electrodes, the magnitude of early cell attachment increased with initial cell density. However, the first cells to attach did not demonstrate the same electron transfer rates as cells grown on electrodes. For example, following initial attachment of fumarate-grown cells, the electron transfer rate was 2 mA/mg protein, but increased to nearly 8 mA/mg protein within 6 h of growth. Once attached, all biofilms grew at a constant rate (doubling every 6 h), and sustained a high specific electron transfer rate and growth yield, while current density was below 300 mA/cm 2 . Beyond this point, the rate of current increase slowed and approached a stable plateau. At all phases, slow scan rate cyclic voltammetry of G. sulfurreducens showed a similar well-defined sigmoidal catalytic wave, indicating the general model of electron transfer to the electrode was not changing. Short-term exposure to reducing potentials (3 h) did not alter these characteristics, but did cause accumulation of electrons which could be discharged at potentials above À 0.1 V. Sustained growth at lower potentials (À 0.16 V) only slightly altered the pattern of detectable redox species at the electrode, but did eliminate this pattern of discharge from the biofilm. Single-turnover voltammetry of colonized electrodes showed at least 3 redox couples at potentials similar to other recent observations, with redox protein coverage of the electrode on the order of ca. 1 nmol/cm 2 . The consistent electrochemistry, growth rate, and growth yield of the G. sulfurreducens biofilm at all stages suggests an initial phase where cells must optimize attachment or electron transfer to a surface, and that after this point, the rate of electron production by cells (rate electrons are delivered to the external surface) remains rate limiting compared to the rate electrons can be transferred between cells, and to electrodes.
A simple one-pot green chemical method for the biosynthesis of gold nanoparticles (AuNPs) by reducing chloroauric acid (HAuCl 4 ) with protein extract of Rhizopus oryzae to produce novel gold nano-bioconjugates (AuNBC) is described. AuNBCs, having sizes ranging from 5 to 65 nm, were synthesized by altering the HAuCl 4 -protein extract ratio. The conjugates were characterized by spectroscopic, electron microscopic, light scattering and electrophoretic mobility measurements. It was found that the aqueous AuNBC suspensions exhibited excellent stability over a wide range of ionic strength, pH and temperature. The effect of pH and ionic strength indicated that stabilization is due to electrostatic repulsion arising from the negative charge of the conjugate proteins. The AuNBCs were stable at temperatures lower than the denaturation temperature of the fungal proteins. The catalytic activity of the as-synthesized AuNBCs was quantified by analysing the reduction of p-nitrophenol by borohydride. The conjugates exhibited interesting size and shape dependent catalytic activity, which was stronger than that observed for AuNPs prepared by conventional chemical methods. The catalytic activity was found to be sensitive to both the surface-area-to-volume ratio and the thickness of the protein coating on the NP. † Electronic supplementary information (ESI) available: Synthesis and characterization details of gold nanoparticles using XPS, UV-vis spectroscopy and TEM (Tables S1-S2, Fig. S1-S5).
Bacteria able to transfer electrons to conductive surfaces are of interest as catalysts in microbial fuel cells, as well as in bioprocessing, bioremediation, and corrosion. New procedures for immobilization of Geobacter sulfurreducens on graphite electrodes are described that allow routine, repeatable electrochemical analysis of cell-electrode interactions. Immediately after immobilizing G. sulfurreducens on electrodes, electrical current was obtained without addition of exogenous electron shuttles or electroactive polymers. Voltammetry and impedance analysis of pectin-immobilized bacteria transferring electrons to electrode surfaces could also be performed. Cyclic voltammetry of immobilized cells revealed voltage-dependent catalytic current similar to what is commonly observed with adsorbed enzymes, with catalytic waves centered at -0.15 V (vs. SHE). Electrodes maintained at +0.25 V (vs. SHE) initially produced 0.52 A/m(2) in the presence of acetate as the electron donor. Electrical Impedance Spectroscopy of coatings was also consistent with a catalytic mechanism, controlled by charge transfer rate. When electrodes were maintained at an oxidizing potential for 24 h, electron transfer to electrodes increased to 1.75 A/m(2). These observations of electron transfer by pectin-entrapped G. sulfurreducens appear to reflect native mechanisms used for respiration. The ability of washed G. sulfurreducens cells to immediately produce electrical current was consistent with the external surface of this bacterium possessing a pathway linking oxidative metabolism to extracellular electron transfer. This electrochemical activity of pectin-immobilized bacteria illustrates a strategy for preparation of catalytic electrodes and study of Geobacter under defined conditions.
In recent years, there has been significant progress in the biological synthesis of nanomaterials. However, the molecular mechanism of gold biomineralization in microorganisms of industrial relevance remains largely unexplored. Here we describe the biosynthesis mechanism of gold nanoparticles (AuNPs) in the fungus Rhizopus oryzae . Reduction of AuCl(4)(-) [Au(III)] to nanoparticulate Au(0) (AuNPs) occurs in both the cell wall and cytoplasmic region of R. oryzae . The average size of the as-synthesized AuNPs is ~15 nm. The biomineralization occurs through adsorption, initial reduction to Au(I), followed by complexation [Au(I) complexes], and final reduction to Au(0). Subtoxic concentrations (up to 130 μM) of AuCl(4)(-) in the growth medium increase growth of R. oryzae and induce two stress response proteins while simultaneously down-regulating two other proteins. The induction increases mycelial growth, protein yield, and AuNP biosynthesis. At higher Au(III) concentrations (>130 μM), both mycelial and protein yield decrease and damages to the cellular ultrastructure are observed, likely due to the toxic effect of Au(III). Protein profile analysis also confirms the gold toxicity on R. oryzae at high concentrations. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis shows that two proteins of 45 and 42 kDa participate in gold reduction, while an 80 kDa protein serves as a capping agent in AuNP biosynthesis.
We report visible spectroelectrochemical (SEC) characterization of cytochrome c 552 respectively. This is the first study to observe the reversible redox conversion of cyt c 552 in viable G. sulfurreducens biofilms.
Enterococci are important human commensals and significant opportunistic pathogens. Biofilm-related enterococcal infections, such as endocarditis, urinary tract infections, wound and surgical site infections, and medical device-associated infections, often become chronic upon the formation of biofilm. The biofilm matrix establishes properties that distinguish this state from free-living bacterial cells and increase tolerance to antimicrobial interventions. The metabolic versatility of the enterococci is reflected in the diversity and complexity of environments and communities in which they thrive. Understanding metabolic factors governing colonization and persistence in different host niches can reveal factors influencing the transition to biofilm pathogenicity. Here, we report a form of iron-dependent metabolism for Enterococcus faecalis where, in the absence of heme, extracellular electron transfer (EET) and increased ATP production augment biofilm growth. We observe alterations in biofilm matrix depth and composition during iron-augmented biofilm growth. We show that the ldh gene encoding l-lactate dehydrogenase is required for iron-augmented energy production and biofilm formation and promotes EET.
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