Hydroalcoholic extract of Rhodiola rosea L. (Crassulaceae) and its hydrolysate inhibit melanogenesis in B16F0 cells by regulating the CREB/MITF/tyrosinase pathway

Chiang, Hsiu-Mei; Chien, Yin Chih; Wu, Chieh Hsi; Kuo, Yueh Hsiung; Wu, Wen-Lan; Pan, Yu Yun; Su, Yu Han; Wen, K.-C.

doi:10.1016/j.fct.2013.12.032

Cited by 72 publications

(63 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The acetone extract of R. rosea exhibits tyrosinase inhibitory activity [48]. Besides R. rosea root containing phenylethanol derivatives, phenylpropanoids, monoterpenes, flavonoids, phenolic acids, and triterpenes [49,50] decreases melanin content in B16 melanoma cells via suppressing the expressions of MC1R, a receptor of a-MSH, MITF, TRP-1, and tyrosianse [51].…”

Section: Regulator On Expression Of Melanogenic Enzymesmentioning

confidence: 99%

Effect of quercetin derivatives on melanogenesis stimulation of melanoma cells

Mitsunaga

Yamauchi

2015

J Wood Sci

View full text Add to dashboard Cite

Controlling melanogenesis is important for maintaining the good health and cosmetic appearance of a human body. This study aims to search the active compounds from natural products exhibiting the melanogenesis modulating activity and elucidate the mechanism underlying the observed activity. Two novel quercetin glycosideswere isolated and identified from Helminthostachys zeylanica roots 50 % ethanol extract. Compound 1 exhibited intracellular melanogenesis stimulatory activity, while 2 showed no effect even the structural similarity. To understand the structure-activity relationships, twelve quercetin glycosides and seven methylquercetins were synthesized from rutin as starting material. As the result of bioassay using synthesized nineteen quercetin derivatives in B16 melanoma cells, some quercetin-3-O-b-D-glucopyranosides stimulated the intracellular melanogenesis. On the other hand, synthesized 3-O-methylquercetin 12 and 3,4 0 ,7-Otrimethylquercetin 15 increased both intra and extracellular melanin contents with no cytotoxicity. Compoud 15 increased the phosphorylated p38 mitogen-activated protein kinase (MAPK) and microphthalmia-associated transcription factor (MITF) which regulates the expression of tyrosinase, TRP-1 and TRP-2. While 12 enhanced the expression of the melanogenic enzymes without involving the MITF, as evidenced by its lack of any stimulation of the expression of MITF and p-p38 MAPK. This result indicates that 12 may stimulate the expression of tyrosinase, TRP-1, and TRP-2 by stimulating currently unidentified transcriptional factors and/or by regulating the degradation of melanogenic enzymes.

show abstract

Section: Regulator On Expression Of Melanogenic Enzymesmentioning

confidence: 99%

Effect of quercetin derivatives on melanogenesis stimulation of melanoma cells

Mitsunaga

Yamauchi

2015

J Wood Sci

View full text Add to dashboard Cite

show abstract

“…Rhodiola rosea B16F0 cells 1 Suppressed melanin synthesis and tyrosinase activity 2 Inhibited TRP-1 and tyrosinase expression 3 Inhibited MC1R/ CREB/ GSK3b/MITF [74,75] Rhodiola rosea extract/ L-carnosine-associated compound Human skin 1 Reduced transepidermal water loss (TEWL) 2 Elevated the skin barrier function 3 Positive subjective response [65] Rhodiola rosea extract/ L-carnosine-associated compound Keratinocytes 1 Promoted the release of proopiomelanocortin peptides 2 Restored to normal the increased levels of neuropeptides and cytokines [65] Rhodiola imbricata SD rats 1 Promoted wound healing 2 Promoted cellular proliferation 3 Increased collagen synthesis 4 Increased the DNA, protein, hydroxyprolin, and hexosamine in granulation cells 5 Antioxidant and decrease lipid peroxidation [38] Rhodiola crenulata extract Propionibacterium acnes antibiofilm activity against Propionibacterium acnes [66] Salidroside B16F0 cells Suppressed melanin synthesis and tyrosinase activity, but no effect on the expression of MC1R, MITF, TRP-1, or TRP-2 [74,78,79] Tyrosol B16F0 cells 1 Suppression of melanin synthesis and tyrosinase activity 2 Suppressed the expression of MC1R and TRP-1, but not TRP-2 and MITF [78] j o u r n a l o f f o o d a n d d r u g a n a l y s i s x x x ( 2 0 1 5 ) 1 e1 1 inhibition of HIF-1a [52]. Rhodiola sachalinensis and salidroside protected liver tissue from oxidative-stress-induced damage.…”

Section: Plants/constituents Models Results Referencesmentioning

confidence: 99%

Rhodiola plants: Chemistry and biological activity

Chiang

Chen

et al. 2015

Journal of Food and Drug Analysis

Self Cite

157

View full text Add to dashboard Cite

Rhodiola is a genus of medicinal plants that originated in Asia and Europe and are used traditionally as adaptogens, antidepressants, and anti-inflammatory remedies. Rhodiola plants are rich in polyphenols, and salidroside and tyrosol are the primary bioactive marker compounds in the standardized extracts of Rhodiola rosea. This review article summarizes the bioactivities, including adaptogenic, antifatigue, antidepressant, antioxidant, anti-inflammatory, antinoception, and anticancer activities, and the modulation of immune function of Rhodiola plants and its two constituents, as well as their potential to prevent cardiovascular, neuronal, liver, and skin disorders.

show abstract

“…This further suggests that R. crenulata can reduce the aggressive potential of melanoma tumors in vivo. While R. crenulata has previously been used topically to reduce melanotic lesions [39], our study is the first one to suggest that a topical application may alter and potentially improve melanoma outcomes. The endpoint for our in vivo subcutaneous studies was based on the length and width of the pigmented area, but not on the vertical axis in tumor volume measurements.…”

Section: Discussionmentioning

confidence: 96%

Antineoplastic effects of Rhodiola crenulata treatment on B16-F10 melanoma

et al. 2015

View full text Add to dashboard Cite

Melanoma is an aggressive form of skin cancer with limited treatment options for advanced stage disease. Early detection and wide surgical excision remain the initial mode of treatment for primary tumors thus preventing metastases and leading to improved prognosis. Through this work, we have evaluated the antineoplastic effects of Rhodiola crenulata (R. crenulata) root extracts on the B16-F10 melanoma cell line, both in vitro and in vivo. We observed that R. crenulata treatment resulted in increased cell death as well as a reduction in tumor cell proliferation and migration in vitro. Additionally, we observed that R. crenulata decreased the expression of integrin β1 and vimentin and increased the expression of E-cadherin. Further, in mice treated with a topical R. crenulata-based cream therapy, tumors were more likely to have a radial growth pattern, a reduction in mitotic activity, and an increase in tumor necrosis. We also observed that mice drinking water supplemented with R. crenulata displayed a reduction of metastatic foci in disseminated models of melanoma. Collectively, these findings suggest that R. crenulata exhibits striking antitumorigenic and antimetastatic properties and that this extract may harbor potential novel adjuvant therapy for the treatment of melanoma.

show abstract

Hydroalcoholic extract of Rhodiola rosea L. (Crassulaceae) and its hydrolysate inhibit melanogenesis in B16F0 cells by regulating the CREB/MITF/tyrosinase pathway

Cited by 72 publications

References 37 publications

Effect of quercetin derivatives on melanogenesis stimulation of melanoma cells

Effect of quercetin derivatives on melanogenesis stimulation of melanoma cells

Rhodiola plants: Chemistry and biological activity

Antineoplastic effects of Rhodiola crenulata treatment on B16-F10 melanoma

Contact Info

Product

Resources

About