Antineoplastic effects of Rhodiola crenulata treatment on B16-F10 melanoma

Dudek, M; Wong, Kaitlyn E.; Bassa, Lotfi M.; Mora, Maria Carmen; Ser‐Dolansky, Jennifer; Henneberry, Jean; Crisi, Giovanna M.; Arenas, Richard B.; Schneider, Sallie S.

doi:10.1007/s13277-015-3742-2

Cited by 12 publications

(8 citation statements)

References 33 publications

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“…Talin regulates the stability of E-cadherin transcriptional repressors [58]. An increased expression of E-cadherin was observed after reducing the level of β 1 -integrin in B16-F10 melanoma cells [59]. Treatment with recombinant MMP-9 or transient expression of MMP-9 is sufficient to reduce E-cadherin levels in differentiated ovarian tumor cells [60].…”

Section: Discussionmentioning

confidence: 99%

Real Microgravity Influences the Cytoskeleton and Focal Adhesions in Human Breast Cancer Cells

Nassef

Kopp

Wehland

et al. 2019

IJMS

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With the increasing number of spaceflights, it is crucial to understand the changes occurring in human cells exposed to real microgravity (r-µg) conditions. We tested the effect of r-µg on MCF-7 breast cancer cells with the objective to investigate cytoskeletal alterations and early changes in the gene expression of factors belonging to the cytoskeleton, extracellular matrix, focal adhesion, and cytokines. In the Technische Experimente unter Schwerelosigkeit (TEXUS) 54 rocket mission, we had the opportunity to conduct our experiment during 6 min of r-µg and focused on cytoskeletal alterations of MCF-7 breast cancer cells expressing the Lifeact-GFP marker protein for the visualization of F-actin as well as the mCherry-tubulin fusion protein using the Fluorescence Microscopy Analysis System (FLUMIAS) for fast live-cell imaging under r-µg. Moreover, in a second mission we investigated changes in RNA transcription and morphology in breast cancer cells exposed to parabolic flight (PF) maneuvers (31st Deutsches Zentrum für Luft- und Raumfahrt (DLR) PF campaign). The MCF-7 cells showed a rearrangement of the F-actin and tubulin with holes, accumulations in the tubulin network, and the appearance of filopodia- and lamellipodia-like structures in the F-actin cytoskeleton shortly after the beginning of the r-µg period. PF maneuvers induced an early up-regulation of KRT8, RDX, TIMP1, CXCL8 mRNAs, and a down-regulation of VCL after the first parabola. E-cadherin protein was significantly reduced and is involved in cell adhesion processes, and plays a significant role in tumorigenesis. Changes in the E-cadherin protein synthesis can lead to tumor progression. Pathway analyses indicate that VCL protein has an activating effect on CDH1. In conclusion, live-cell imaging visualized similar changes as those occurring in thyroid cancer cells in r-µg. This result indicates the presence of a common mechanism of gravity perception and sensation.

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Section: Discussionmentioning

confidence: 99%

Real Microgravity Influences the Cytoskeleton and Focal Adhesions in Human Breast Cancer Cells

Nassef

Kopp

Wehland

et al. 2019

IJMS

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“…The anticancer activity of another R. crenulata extract (Extract 6 ) has been investigated on U87 GBM cells, which involves the inhibition of Wnt‐β‐catenin signaling . In addition, Extract 8 could induce cell death and antiproliferation effect in B16‐F10 mouse melanoma cells, and inhibit metastatic melanoma in a mouse model . A 96% ethanol extract of R. rosea (Extract 9 ) could induce apoptosis and necrosis in HL‐60 cells by cell cycle arrest in G1 phase .…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

“…143 In addition, Extract 8 could induce cell death and antiproliferation effect in B16-F10 mouse melanoma cells, and inhibit metastatic melanoma in a mouse model. 145 A 96% ethanol extract of R. rosea (Extract 9)…”

Section: Anticancer Activitymentioning

confidence: 99%

Rhodiola species: A comprehensive review of traditional use, phytochemistry, pharmacology, toxicity, and clinical study

Tao

Cao

et al. 2019

Medicinal Research Reviews

106

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Rhodiola species, belonging to the family Crassulaceae, have long been used as an adaptogen, tonic, antidepressant, and antistress medicine or functional food in Asia and Europe. Due to the valuable application, the growing demand of Rhodiola species has led to a rapid decrease in resource content. This review aims to summarize the integrated research progress of seven mainstream Rhodiola species. We first outline both traditional and current use of Rhodiola for the treatment of various diseases. A detailed summary and comparison of chemical, pharmacological, toxicological, and clinical studies of various Rhodiola species highlight recent scientific advances and gaps, which gives insights into the understanding of Rhodiola application and would be helpful to improve the situation of biological resources and diversities of Rhodiola plants.

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“…In this study, we found that ICA could inhibit cell proliferation and reduced colony formation in a concentration- and time-dependent manner. This is important because uninhibited migration of melanoma leads to aggressive phenotype and high mortality rates of patients [ 20 ]. So, we carried out further experiments to explore the mechanism of ICA on B16 cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Icariin induces cell differentiation and cell cycle arrest in mouse melanoma B16 cells via Erk1/2-p38-JNK-dependent pathway

Wang

Chen

et al. 2017

Oncotarget

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Icariin (ICA) is a major component isolated from Epimedium brevicornum. Emerging evidence shows that ICA can inhibit tumor cell proliferation, invasion and migration. However, the anti-cancer effect of ICA on B16 cells has not been fully investigated. Here we found that the proliferation of B16 cells was inhibited by ICA in a concentration- and time-dependent manner, and the colony formation of B16 cells was also inhibited by ICA in a concentration-dependent manner. Further study showed that the melanin content was increased and the tyrosinase (Tyr) activity was enhanced after ICA treatment in B16 cells. Furthermore, compared with the control group, the mRNA levels of Tyr, Trp1 and Trp2 and the protein level of MITF were increased in ICA-treated B16 cells. In addition, the percentage of G0/G1 phase cells was increased and the protein levels of Cyclin A, CDK2 and p21 were decreased in ICA-treated B16 cells. Finally, we found that ICA increased down-regulated the Erk1/2, p-Erk1/2, p38, p-p38, and p-JNK protein levels in B16 cells when compared with the control group. Taken together, these results indicated that ICA could induce B16 cell differentiation and cell cycle arrest at G0/G1 phase through inhibiting Erk1/2-p38-JNK-dependent signaling molecules.

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Antineoplastic effects of Rhodiola crenulata treatment on B16-F10 melanoma

Cited by 12 publications

References 33 publications

Real Microgravity Influences the Cytoskeleton and Focal Adhesions in Human Breast Cancer Cells

Real Microgravity Influences the Cytoskeleton and Focal Adhesions in Human Breast Cancer Cells

Rhodiola species: A comprehensive review of traditional use, phytochemistry, pharmacology, toxicity, and clinical study

Icariin induces cell differentiation and cell cycle arrest in mouse melanoma B16 cells via Erk1/2-p38-JNK-dependent pathway

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