Hyaluronidase Enzyme-responsive Targeted Nanoparticles for Effective Delivery of 5-Fluorouracil in Colon Cancer

Artificial Cells, Nanomedicine, and Biotechnology

Almohazey

et al. 2019

Poly (methyl methacrylate) (PMMA) is basically biocompatible polyester with high resistance to chemical hydrolysis, and high drug permeability and the most important characteristics of PMMA is that it does not produce any toxicity. There is not much information about PMMA action on the colon cancer cells. In the present study, we have synthesized PMMA nanoparticles. The distribution pattern of PMMA particles was analysed by Zeta sizer and the size of the particles was calculated by using quasi elastic light scattering (QELS). The surface structure and the morphology of PMMA were characterized by transmission electron microscope (TEM) and scanning electron microscope (SEM), respectively. We have also analysed their effects on cancerous cells (human colorectal carcinoma cells, HCT-116) and normal, healthy cells (human embryonic kidney cells, HEK-293) by using morphometric, MTT, DAPI and wound healing methods. We report that PMMA particles inhibited the cancer cell viability in a dose-dependent manner. The lower dose (1.0 lg/ml) showed a moderate decrease in cancer cell viability, whereas higher dosages (2.5 lg/ml, 5.0 lg/mL and 7.5 lg/mL) showed steadily decrease in the cancer cell viability. We also report that PMMA is highly selective to cancerous cells (HCT-116), as we did not find any action on the normal healthy cells (HEK-293). In conclusion, our results suggest PMMA particles are potential biomaterials to be used in the treatment of colon cancer.

Section: Introductionmentioning

confidence: 99%

Targeted delivery of poly (methyl methacrylate) particles in colon cancer cells selectively attenuates cancer cell proliferation

Artificial Cells, Nanomedicine, and Biotechnology

Almohazey

et al. 2019

“…According to the knowledge of the authors, the deep penetration of MNPs into the HCT‐116 cells is not explored previously by any research group using TEM. There are only a few reports available where penetration of NPs into the cancerous cells is discussed (Jiang, He, et al, ; Jiang, Shi, et al, ; Jie, Yuan, Huang, & Zhao, ; Mahmoudi et al, ; Mi et al, ; Montagne et al, ; Pati, Das, Mehta, Sahu, & Sonawane, ; Rasmussen, Martinez, Louka, & Wingettt, ; Smalley & Herlyn, ; Turcotte et al, ; Unfried et al, ; Xu et al, ).…”

Section: Introductionmentioning

confidence: 99%

Functionalized magnetic nanoparticles attenuate cancer cells proliferation: Transmission electron microscopy analysis

Microscopy Res & Technique

Buhaimed

2019

The penetration and transportation of nanoparticles (NPs) inside the cancer cells is critical to study. In this article, cancer cells (HCT-116) were treated with functionalized magnetic NPs for the period of 48 hr and studied their ultrastructure by transmission electron microscopy (TEM).The NPs-treated cells were prepared by chemical fixation and sliced into electron-transparent arbitrary sections (200 × 200 μm 2 ) by ultramicrotome. Major events of NPs-cell interaction, such as penetration of NPs, encapsulation of NPs into the intracellular compartments, transportation of NPs, and NPs exit, were examined by TEM to understand the mechanism of cell death. The NPs showed the uniform spherical shape with broad size distribution (100-400 nm), while cells displayed irregular morphology with average diameter~5 μm. Our results showed the successful penetration of NPs deep into the cell, encapsulation, transportation, and exocytosis. Furthermore, we tested the different concentrations (0, 1.5, 12.5, and 50 μg/ml) of NPs on cancer cells and evaluated the cell viability. Laser confocal microscopy and colorimetric analysis together demonstrated that the cell viability is a dose-dependent phenomenon, where 50 μg/ml specimen showed the highest killing of cancer cells compared to other dosages. K E Y W O R D S arbitrary sections, cancer cells, magnetic nanoparticles, nanoparticle-cell interaction, TEM

“…In recent past, several attempts have been made to study the effect of different types of nanoparticles on cancer treatments. Most of the studies suggest that nanoparticles possess and exhibit anti-cancer properties [4,5,[9][10][11][12][13][14][15][16][17][18], also in being tested in colon cancer [19]. Interestingly, all these nanoparticles have shown anti-cancer properties with mostly quantifiable manner, but still there is no report about the use of both qualitative and quantitative methods to evaluate the nanoparticles effect on cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent magnetic submicronic polymer (FMSP) nanoparticles induce cell death in human colorectal carcinoma cells

Artificial Cells, Nanomedicine, and Biotechnology

Almohazey

et al. 2018

Nanoparticles have many advantages such as high biocompatibility, bioavailability and effective therapeutic capabilities. The aim of the present study is to examine whether fluorescent magnetic submicronic polymer nanoparticles (FMSP-nanoparticles) have any impact on human colorectal cancer cells. In the present study, we have tested FMSP-nanoparticles with an average size of 100-200 nm on human colorectal carcinoma cells (HCT-116) to check their cytotoxic and anti-cancer capabilities. The effects of FMSP-nanoparticles on cancer cells were observed after 6 h, 24 h and 48 h intervals. The response of FMSP-nanoparticles-treated cells was evaluated by Trypan Blue, 4lue-diamidino-2-phenylindole (DAPI) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Our MTT analysis results revealed that FMSP-nanoparticles produced dose-dependent effects on cancer cells, FMSP-nanoparticles with dose of 1.25 µg/mL did not decrease cell survivability, whereas dosages of 12.5 µg/mL and 50 µg/mL respectively showed 23.59% and 59.47% decrease in the cancer cell survivability. In conclusion, our results demonstrate FMSP-nanoparticles have a potential anti-cancer capability and hold a great promise for colon cancer treatments.