Targeted delivery of poly (methyl methacrylate) particles in colon cancer cells selectively attenuates cancer cell proliferation

Abstract

Poly (methyl methacrylate) (PMMA) is basically biocompatible polyester with high resistance to chemical hydrolysis, and high drug permeability and the most important characteristics of PMMA is that it does not produce any toxicity. There is not much information about PMMA action on the colon cancer cells. In the present study, we have synthesized PMMA nanoparticles. The distribution pattern of PMMA particles was analysed by Zeta sizer and the size of the particles was calculated by using quasi elastic light scattering (QELS). The surface structure and the morphology of PMMA were characterized by transmission electron microscope (TEM) and scanning electron microscope (SEM), respectively. We have also analysed their effects on cancerous cells (human colorectal carcinoma cells, HCT-116) and normal, healthy cells (human embryonic kidney cells, HEK-293) by using morphometric, MTT, DAPI and wound healing methods. We report that PMMA particles inhibited the cancer cell viability in a dose-dependent manner. The lower dose (1.0 lg/ml) showed a moderate decrease in cancer cell viability, whereas higher dosages (2.5 lg/ml, 5.0 lg/mL and 7.5 lg/mL) showed steadily decrease in the cancer cell viability. We also report that PMMA is highly selective to cancerous cells (HCT-116), as we did not find any action on the normal healthy cells (HEK-293). In conclusion, our results suggest PMMA particles are potential biomaterials to be used in the treatment of colon cancer.

UsingFomitopsis pinicolafor bioinspired synthesis of titanium dioxide and silver nanoparticles, targeting biomedical applications

“…Interestingly, there are several reports of NPs, which are known to induce fragmentation and disintegration of nucleus in cancer cells. [43][44][45]…”

Elemental analysis of Moringa oleifera seeds by Laser Induced Breakdown Spectroscopy (LIBS) and its anti-cancer and anti-microbial studies

“…In this study, the cancerous and normal (healthy) cell line including the respective human colorectal carcinoma (HCT-116) and embryonic kidney (HEK-293) cells were used to evaluate the anti-cancerous activity of various MOSs extracts on these cells. Following the earlier prescribed method [36,37], the proposed cells were seeded in 96 well plates and grown in the Dulbecco’s Modified Eagle Medium (DMEM) supplemented with the reagents such as selenium chloride, L-glutamine, fetal bovine serum, antibiotic penicillin and streptomycin. One group of these cells were cultured inside a CO 2 incubator at the temperature of 37 °C and treated at various concentrations (0.030 mg/ml to 0.1 mg/ml) of the MOSs extracts.…”

A Review of Functional Separators for Lithium Metal Battery Applications

“…Reprinted with permission from [ 57 ]. Copyright (2008) Elsevier B.V. ( e ) PMMA particles Reprinted with permission from [ 58 ] Copyright (2018) CC BY. ( f ) PAN nanofibers Reprinted with permission from [ 59 ] Copyright (2019) CC BY.…”