Hyaluronidase decreases neutrophils infiltration to the inflammatory site

Muhr, Cornélia; Silveira, Denise Sayuri Calheiros da; Sorgi, Carlos Artério; Rodrigues, S.; Farsky, Sandra Helena Poliselli; Paula-Silva, Francisco Wanderley Garcia; Merfort, Irmgard; Faccioli, Lúcia Helena

doi:10.1007/s00011-016-0935-0

Cited by 26 publications

(22 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The subcutaneous mouse air pouch was produced as previously described [24,25]. Briefly, before creating the air pouch, the animals were anesthetized (i. p.) with ketamine (80 mg/kg) and xylazine (15 mg/kg).…”

Section: In Vivo Air Pouch Induction and Experimental Designmentioning

confidence: 99%

“…Controls received sterile PBS only (0.5 mL/pouch). The selected doses were chosen based on our in vitro experiments and previous published works using plant extracts [10,[25][26][27][28]. After 4 or 24 h, the animals were euthanized ▶ Fig.…”

Section: In Vivo Air Pouch Induction and Experimental Designmentioning

confidence: 99%

“…Aliquots were separated for a manual leukocyte count using Turkʼs solution in a Neubauer chamber. Differential leukocyte counts were performed on cytospin preparations stained by using the kit for fast staining in hematology (Fast Panoptic -Instant Prov) [25]. The remaining exudate was centrifuged at 400 g for 10 min at 4°C, and the cell-free supernatant was stored at − 70°C for protein, nitrite, and cytokine measurements.…”

Section: Air Pouch Exudates Collection and Evaluation Of Leukocyte Acmentioning

confidence: 99%

“…Total proteins were quantified in the cell-free lavage fluid obtained from the air pouch cavities, as previously described [25,29]. Experiments were performed in a 96-well plate and the protein concentration was calculated by regression analysis of a standard curve using bovine serum albumin (BSA) by colorimetric measurements at 595 nm in an ELISA microplate reader (Molecular Devices Spectra MAX 190).…”

Section: Protein Quantificationmentioning

confidence: 99%

See 3 more Smart Citations

In Vitro and In Vivo Anti-inflammatory Effects of Struthanthus vulgaris

et al. 2017

Self Cite

View full text Add to dashboard Cite

is probably the most common medicinal mistletoe plant in Brazil, and has been used in folk medicine as an anti-inflammatory agent and for cleaning skin wounds. Our proposal was to evaluate the anti-inflammatory activity of ethanol leaf extract and provide further insights of how this biological action could be explained using and assays. anti-inflammatory activity was preliminarily investigated in lipopolysaccharide/interferon gamma-stimulated macrophages based on their ability to inhibit nitric oxide production and tumor necrosis factor-alpha. anti-inflammatory activity of ethanol leaf extract was investigated in the mice carrageenan-induced inflammation air pouch model. The air pouches were inoculated with carrageenan and then treated with 50 and 100 mg/kg of ethanol leaf extract or 1 mg/kg of dexamethasone. Effects on the immune cell infiltrates, pro- and anti-inflammatory mediators such as tumor necrosis factor-alpha, interleukin 1, interleukin 10, and nitric oxide, were evaluated. The chemical composition of ethanol leaf extract was characterized by LC-MS/MS. ethanol leaf extract significantly decreased the production of nitric oxide and tumor necrosis factor-alpha in macrophages and did not reveal any cytotoxicity. ethanol leaf extract significantly suppressed the influx of leukocytes, mainly neutrophils, protein exudation, nitric oxide, tumor necrosis factor-alpha, and interleukin 1 concentrations in the carrageenan-induced inflammation air pouch. In conclusion, ethanol leaf extract exhibited prominent anti-inflammatory effects, thereby endorsing its usefulness as a medicinal therapy against inflammatory diseases, and suggesting that ethanol leaf extract may be a source for the discovery of novel anti-inflammatory agents.

show abstract

Section: In Vivo Air Pouch Induction and Experimental Designmentioning

confidence: 99%

Section: In Vivo Air Pouch Induction and Experimental Designmentioning

confidence: 99%

Section: Air Pouch Exudates Collection and Evaluation Of Leukocyte Acmentioning

confidence: 99%

Section: Protein Quantificationmentioning

confidence: 99%

See 2 more Smart Citations

In Vitro and In Vivo Anti-inflammatory Effects of Struthanthus vulgaris

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Com o auxílio de uma pipeta os lavados foram coletados, através de uma incisão na bolsa. Os lavados foram utilizados para contagem total e diferencial de células, quantificação de proteínas e citocinas(FRONZA et al, 2016).…”

unclassified