Hyaluronic acid (sperm select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens

Huszár, Gábor; Willetts, Melanie; Corrales, Marcelia

doi:10.1016/0020-7292(91)90748-t

Cited by 32 publications

(41 citation statements)

References 20 publications

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“…Thus, the conclusion was that the tubal fluid in the pre-ovulatory functional sperm reservoir delayed capacitation in vivo rather than promote it. In vitro, hyaluronan improves sperm motility in a dose-dependent manner [31,32]. Hyaluronan has also been considered as a proper additive for cryopreservation, mostly owing to its stabilizing features on the plasma membrane [33], or its capacity as an antioxidant [34,35].…”

Section: The Stored Tubal Spermatozoa and Their Relation To The Tubalmentioning

confidence: 99%

The ubiquitous hyaluronan: Functionally implicated in the oviduct?

et al. 2016

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Hyaluronan (hyaluronic acid) is a simple, non-antigenic, non-sulphated glycosaminoglycan (GAG) present everywhere in the extracellular compartments of the body. Noteworthy, it is highly conserved phylogenetically, from sauropsida to mammals; and plays a plethora of roles from embryonic/fetal development to adult physiological and pathological events, including tumor development. In reproduction, hyaluronan has proven related to initial events as sperm survival, build-up of the sperm reservoir in the oviduct, regulation of sperm capacitation and pre-fertilization to later participate in embryo, fetal and placental development. Synthesis, binding (via the CD44 membrane receptor) and degradation of hyaluronan occur in male and female genital organs, the oviduct being no exception. This review discusses our current knowledge on roles of this ubiquitous GAG on the survival of immunologically foreign spermatozoa in the pig oviduct, a relevant event for fertility. During pre-ovulatory storage in the functional tubal sperm reservoir, spermatozoa are entrapped in a mucus-like tubal fluid.This fluid contains fluctuating levels of hyaluronan, which is synthesized by the lining 18th ICAR-2016-S2. Pig reproduction Symp 2 epithelium by HA-synthase 3 (has3). Both hyaluronan and its CD44-receptor are particularly evident in the deep mucosal furrows of the sperm reservoir, where most spermatozoa are embedded in; kept alive, un-capacitated but also undetected by the immune system of the female. Hyaluronan is also present in the seminal plasma, and evidence points out an involvement of hyaluronan and its receptor in the local (tubal and possibly uterine) production of anti-inflammatory cytokines, such as IL-10, pertaining maternal immune tolerance of these foreign cells.

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Section: The Stored Tubal Spermatozoa and Their Relation To The Tubalmentioning

confidence: 99%

The ubiquitous hyaluronan: Functionally implicated in the oviduct?

et al. 2016

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“…In addition, the sperm reservoir controls the progression of suitable spermatozoa, in very restricted numbers, to the site of fertilization of the newly ovulated oocytes [32,34,38]. Moreover, various GAGs have been found to be able to maintain sperm motility and induce capacitation in vitro in bovine [39] and other species [40][41][42].…”

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confidence: 99%

Detection of the Hyaluronan Receptor CD44 in the Bovine Oviductal Epithelium

et al. 2005

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Abstract. Hyaluronan is involved in fundamental reproductive events such as sperm storage in the female reproductive tract, fertilization, and early embryo development, these functions are presumably mediated by its major cell surface receptor, CD44. The present study was conducted to investigate the presence and localization of CD44 in the bovine oviductal epithelium, using immunohistochemical and Western blot methods on tissue sections and epithelial cell extracts collected from the uterotubal junction (UTJ), isthmus, and ampulla of animals in the oestrus or luteal phase of the oestrous cycle. While positive immunolabelling for CD44 was found on the ad-luminal surface and supra-nuclear region of epithelial cells in all tubal segments investigated, in the UTJ, there were epithelial cells in which the entire cytoplasm positively stained. We found no differences in terms of CD44-positive staining between the different stages of the oestrous cycle. Presence of CD44 was detected by Western blotting in the tubal epithelium as a single band at 200 kDa. Although it appeared in all tubal segments, the expression of CD44 protein was more accentuated in the sperm reservoir (UTJ) than in the other segments. This is the first time CD44 has been detected in the epithelium of the tubal sperm reservoir in cattle, suggesting a pathway for the action of hyaluronan in this segment.

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“…Because of the presence of casein (as natural antioxidant) in milk, as also used herein, this might account for the fact that an array of antioxidants was not beneficial to sperm cells for skimmed milk extender. In the last few years, it has been reported that supplementation of semen extenders by hyaluronic acid maintains good spermatozoal motility in human (12) and goats (15). It was also favourable for maintaining the membrane stability and viability of spermatozoa in boars (20).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, its concentration (1000 µg/ml) used herein was directly adopted from those studies conducted in human and boar semen. Adding hyaluronic acid (1000 µg/ml) to the freezing extender enhanced post-thaw motility of boar and human sperm (11,12). Contradictory reports existing on the effect of antioxidant supplementation on postthaw motility of semen from various species, including the buck (4,8,17,24,25), were resulted from the different the types and doses of the antioxidants employed.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of Saanen buck semen frozen in different extenders supplemented with various antioxidants

Recai;DAŞKIN¹

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of this present study was to investigate the effects of extenders containing different antioxidants upon the principle sperm characteristics of Saanen buck semen following freezing. In this study, four bucks were used. Semen was collected twice a week by an artificial vagina during the breeding season to obtain ten ejaculates per animal. The samples were then extended in three extenders (tris, T-, skimmed milk, M-and laiciphos 488, L-based) without (as controls) or with the two antioxidants (5 mM cysteine, C and 1000 µg/ml hyaluronic acid, H). By this way, nine experimental groups were assigned, as follows:For the T extender groups, the rates of motility and viability were significantly (p<0.01) higher for the T-C extender as compared to those of the T-H and T-O. However, there was no such difference (p>0.05) among the extenders based on the rates of abnormal spermatozoa, abnormal acrosome and hypoosmotic swelling (HOS) test. For the M groups, the motility was significantly (p<0.01) higher for the M-C and M-O extenders as compared to that of the M-H. However, there was no such difference (p>0.05) among the extenders based on the rates of viability, abnormal spermatozoa, abnormal acrosome and HOS test. For the L groups, the rates of motility and viability were significantly (p<0.01) higher for the L-C extender as compared to that of the L-H but not that of the L-O. Additionally, there was no such difference (p>0.05) among the extenders mentioned above based on the rates of abnormal spermatozoa, abnormal acrosome and HOS test. As a result, the present in vitro findings of semen quality parameters studied suggest that; i) both skimmed milk and laiciphos extenders were more favourable than tris, and that ii) the cysteine, as an antioxidant, provided a superior protection than the hyaluronic acid did.

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Hyaluronic acid (sperm select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens

Cited by 32 publications

References 20 publications

The ubiquitous hyaluronan: Functionally implicated in the oviduct?

The ubiquitous hyaluronan: Functionally implicated in the oviduct?

Detection of the Hyaluronan Receptor CD44 in the Bovine Oviductal Epithelium

In vitro evaluation of Saanen buck semen frozen in different extenders supplemented with various antioxidants

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