Hyacinth mosaic virus infecting Hyacinthus sp. plants in Brazil

Alexandre, M. A. V.; Duarte, L. M. L.; Rodrigues, Leilane Karam; Ramos, A. F.; Harakava, Ricardo

doi:10.1007/s40858-016-0121-5

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…Chromosome characteristics and variation in different taxa can be analyzed by cytogenetic research to infer the degree of species evolution and interspecific genetic relationships, and chromosome identification is its basis (Ilnicki 2014, Liu et al 2018. In recent years, research on hyacinth has mostly focused on several aspects, such as biological properties, cultivation, breeding, and secondary metabolism (Miyamoto et al 2015, Hu et al 2015, Alexandre et al 2017, Xie et al 2018. Research on hyacinth chromosomes can be traced back to the 1970s.…”

Section: Introductionmentioning

confidence: 99%

Physical mapping of 45S and 5S rDNA and telomeric repeat loci in eight diploid hyacinth cultivars

SU²,

XU³

et al. 2022

Phytotaxa

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Hyacinth, one of the five best-selling bulbous flowers in the world, is a favored flower of garden landscapes in the spring. Hyacinth has a single origin, but there are many horticultural varieties. Physical mapping of ribosomal DNA (rDNA) and telomeric repeat loci by fluorescence in situ hybridization (FISH) is helpful to understand the genetic relationship and chromosome inheritance in hyacinth breeding. In this study, 45S rDNA, 5S rDNA and telomeric repeats were used as probes, the mitotic metaphase chromosomes of 8 diploid hyacinth cultivars were analyzed by FISH location, and their karyotype parameters were analyzed by Q-type cluster analysis. The results showed that two 45S rDNA loci were detected in all eight hyacinth cultivars, four 5S rDNA loci were detected in ‘Gypsy Queen’, and three were detected in other cultivars. In addition to the presence of telomere loci at both ends of chromosomes, seven telomere loci were detected in ‘Gypsy Princess’ and ‘Yellow Stone’, and eight telomere loci were detected in other cultivars. At the same time, when the euclidean distance between groups was 20, the eight hyacinth cultivars were clustered into three groups (‘Yellow Stone’, ‘Red Pearl’ and ‘Pink Pearl’), and ‘Gypsy Prince’ was a single group; the other four cultivars were in one group. The distribution of 45S rDNA loci in diploid hyacinth cultivars was relatively conservative, while the locus number and distribution of 5S rDNA and telomeric repeats were highly polymorphic. The FISH physical mapping in this study provides a basic understanding of the karyotype evolution of hyacinth species, which could increase the understanding of the evolutionary history and phylogenetic relationships of hyacinths.

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Section: Introductionmentioning

confidence: 99%

Physical mapping of 45S and 5S rDNA and telomeric repeat loci in eight diploid hyacinth cultivars

SU²,

XU³

et al. 2022

Phytotaxa

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“…Potyviruses are also known to infect spring flowering bulbous plants. Currently Hyacinth mosaic virus is known as the only recognized potyvirus species capable of infecting Muscari (Alexandre et al 2017;de Best et al 2000;Derks and Vink-van den Abeele 1980;Pham et al 2011;Sastry et al 2019). Symptoms include light or dark green stripes and flecks on the foliage.…”

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confidence: 99%

Identification and characterization of a new potyvirus infecting Muscari in Hungary

et al. 2020

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Grape hyacinths (Muscari spp.) are popular spring flowering bulbs in Europe and also in Hungary. In the spring of 2017, we came across grape hyacinth plants showing mosaic symptoms, which indicated viral infection. Currently Hyacinth mosaic virus (genus Potyvirus), a proposed member of the genus named Muscari mosaic virus, Arabis mosaic virus (genus Nepovirus), Cucumber mosaic virus (genus Cucumovirus) and Tobacco rattle virus (genus Tobravirus) are known to infect grape hyacinth. Leaf samples of symptomatic grape hyacinths were observed, collected and the presence of potyviruses was proved with potyvirus specific monoclonal antibody by ELISA and by potyvirus specific RT-PCR. Laboratory host plants and seed grown Muscari plants were inoculated with leaf sap and symptoms were recorded. Nucleotide sequences of the cloned fragments were compared to GenBank data. In the case of Muscari ‘Helena’ the highest nucleotide identity in the coat protein coding region was found with two Muscari mosaic virus isolates (95.51% and 95.79%). In the case of the clones derived from Muscari ‘Pink Sunrise’ plant, the highest identity was recorded with Muscari mosaic virus isolates (57.65% and 57.80%) and with a Tulip breaking virus strain (55.13%) indicating the existence of a novel potyvirus species, tentatively named Muscari chlorotic mottle virus. The coat protein sequences were aligned and Maximum Likelihood trees were built to analyze phylogenetic relationships.

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