HuR Promotes the Progression of Gastric Cancer through Mediating CDC5L Expression

Abstract

Background and Objectives. Gastric cancer (GC) is one of the common gastrointestinal tumors and the third in the mortality rate among tumors. Studies have shown that the human antigen receptor (HuR) is associated with the malignant degree of GC. Methods. We performed qRT-PCR, cell cycle assay, cell migration, and mouse transplantation model analysis in our experiments. It has been clarified that HuR and microRNAs (miRNAs) have important interplays in the regulation of tumor progression. Results. This study found microRNA-133b (miR-133b), as a HuR-sponged miRNA in GC cells. Downregulation of HuR can promote the expression of miR-133b and further affect the downstream cyclin CDC5L. The expressions of miR-133b were slightly lower in GC tissues than adjacent normal tissues. Conclusion. Our studies suggest that HuR and miR-133b are involved in the development and pathological process of GC cells.

Matrine inhibits cell proliferation, invasion, and stem cell formation in hepatocellular carcinoma by regulating the ELAVL1/RBM3‐mediated Wnt/β‐catenin signaling pathway

“…In recent years, RBPs have been critical post‐transcriptional regulators of gene expression involved in a wide range of physiological and pathological processes, which has brought new light to the investigation of TCM's mechanisms. As one of the best‐studied RNA‐binding post‐transcriptional regulators, ELAVL1 was universally expressed and evolutionarily conserved to maintain the basic functions of cells and its disorders might cause many types of diseases, containing cancers (Cui et al., 2022; Wu & Xu, 2022; Xiao et al., 2022). Moreover, ELAVL1 overexpression has undoubtedly been associated with an increased risk of tumor growth, progression, and metastasis (Goutas et al., 2022; Wu et al., 2019), implying the potential therapeutic target candidate in human cancers.…”

RNA-binding proteins and exoribonucleases modulating miRNA in cancer: the enemy within

“…In addition to the aforementioned functions, ELAVL1/HuR can either directly or indirectly regulate miRNAs to affect the expression of their target mRNAs. ELAVL1/HuR was shown to promote gastric cancer cell proliferation in vitro and in vivo by negatively regulating miR-133b expression, in turn affecting the expression of the downstream gene CDC5L 83 . Since the detailed mechanisms underlying the negative modulation of miR-133b by ELAVL1/HuR were not clarified in the study, further investigations should be performed to identify the mode of action.…”

MicroRNAs, Tristetraprolin Family Members and HuR: A Complex Interplay Controlling Cancer-Related Processes

“…HuR-miR-133b. In gastric cancer (GC) tissue and cell lines (BGC-823, MKN-45, MGC-803, SGC-7901, and AGS), HuR expression is high and promotes cancer cell proliferation and migration in vitro and in vivo [ 72 ]. This effect is mediated by the ability of HuR to decrease miR-133b expression, which exerts a tumor suppressive function in GC cells.…”

“…Furthermore, HuR stabilizes CDC5L (cell division cycle 5-like protein) mRNA, while miR-133b inhibits its expression. Therefore, the overexpression of CDC5L , which promotes cell cycle progression, results from the overexpression of HuR and the loss of miR-133b in GC cells [ 72 ].…”