Human α-thrombin detection platform using aptamers on a silicon nanowire field-effect transistor

Römhildt, Lotta; Zörgiebel, F.; Ibarlucea, Bergoi; Vahdatzadeh, Maryam; Baraban, Larysa; Cuniberti, Gianaurelio; Pregl, Sebastian; Weber, Walter M.; Mikolajick, Thomas; Opitz, Jörg

doi:10.1109/patmos.2017.8106958

Cited by 3 publications

(4 citation statements)

References 22 publications

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“…The acidity of the solution thus ensures the positively charged state of the protein molecules are able to influence the surface potential of the NW FETs [25,26,47].…”

Section: Detection Of Thrombin: Optimizing the Conditionsmentioning

confidence: 99%

“…They offer many advantages compared to antibodies including higher stability under various conditions or the potential for facile labeling with functional groups and dyes [23,24]. The benefits of the combination of aptamers with FETs for biosensing has been reported by a number of groups, where the FETs are adapted for the detection of various analytes including thrombin [25,26], potassium [27], dopamine [28], and vascular endothelial growth factor [29], among others. Specifically, the detection of human alpha-thrombin has a high clinical relevance as there are diseases correlated with coagulation irregularities with pathological levels in the upper pM and healthy levels in the nM-µM range [30].…”

Section: Introductionmentioning

confidence: 99%

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Gating Hysteresis as an Indicator for Silicon Nanowire FET Biosensors

et al. 2018

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Abstract:We present a biosensor chip with integrated large area silicon nanowire-based field effect transistors (FET) for human α-thrombin detection and propose to implement the hysteresis width of the FET transfer curve as a reliable parameter to quantify the concentration of biomolecules in the solution. We further compare our results to conventional surface potential based measurements and demonstrate that both parameters distinctly respond at a different analyte concentration range. A combination of the two approaches would provide broader possibilities for detecting biomolecules that are present in a sample with highly variable concentrations, or distinct biomolecules that can be found at very different levels. Finally, we qualitatively discuss the physical and chemical origin of the hysteresis signal and associate it with the polarization of thrombin molecules upon binding to the receptor at the nanowire surface.

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“…The acidity of the solution thus ensures the positively charged state of the protein molecules are able to influence the surface potential of the NW FETs [25,26,47].…”

Section: Detection Of Thrombin: Optimizing the Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gating Hysteresis as an Indicator for Silicon Nanowire FET Biosensors

et al. 2018

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“…In recent years, the application of semiconductor field-effect transistors (FET) sensors have attracted a lot of attention because of their ability to translate the interaction with target molecules on the FET surface to an electrical signal directly [ 1 , 2 , 3 , 4 ]. Silicon nanowires (Si NW) sensors have been considered as one of the most promising candidates for biochemical sensors [ 5 , 6 , 7 , 8 , 9 ], due to their large surface to volume (S/V) ratio, high sensitivity, and good biocompatibility [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Low Cost and Low Temperature Poly-Silicon Nanowire Sensor Arrays for Monolithic Three-Dimensional Integrated Circuits Applications

et al. 2020

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In this paper, the poly-Si nanowire (NW) field-effect transistor (FET) sensor arrays were fabricated by adopting low-temperature annealing (600 °C/30 s) and feasible spacer image transfer (SIT) processes for future monolithic three-dimensional integrated circuits (3D-ICs) applications. Compared with other fabrication methods of poly-Si NW sensors, the SIT process exhibits the characteristics of highly uniform poly-Si NW arrays with well-controlled morphology (about 25 nm in width and 35 nm in length). Conventional metal silicide and implantation techniques were introduced to reduce the parasitic resistance of source and drain (SD) and improve the conductivity. Therefore, the obtained sensors exhibit >106 switching ratios and 965 mV/dec subthreshold swing (SS), which exhibits similar results compared with that of SOI Si NW sensors. However, the poly-Si NW FET sensors show the Vth shift as high as about 178 ± 1 mV/pH, which is five times larger than that of the SOI Si NW sensors. The fabricated poly-Si NW sensors with 600 °C/30 s processing temperature and good device performance provide feasibility for future monolithic three-dimensional integrated circuit (3D-IC) applications.

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“…Accordingly, the utilization of immobilized aptamers, as selective and strong-binding receptors, combined with the dissociation-mode regime, can be expected to enable direct sensing of chemical species in undiluted biosamples. While aptamer-modified SiNW-FETs have been utilized for the detection of dopamine, , neuropeptide Y, thrombin, , vascular endothelial growth factor, and potassium ion, none of which demonstrated measurement in undiluted biological fluids.…”

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confidence: 99%

Direct Detection of Uranyl in Urine by Dissociation from Aptamer-Modified Nanosensor Arrays

et al. 2020

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An ever-growing demand for uranium in various industries raises concern for human health of both occupationally exposed personnel and the general population. Toxicological effects related to uranium (natural, enriched, or depleted uranium) intake involve renal, pulmonary, neurological, skeletal, and hepatic damage. Absorbed uranium is filtered by the kidneys and excreted in the urine, thus making uranium detection in urine a primary indication for exposure and body burden assessment. Therefore, the detection of uranium contamination in bio-samples (urine, blood, saliva, etc.,) is of crucial importance in the field of occupational exposure and human health-related applications, as well as in nuclear forensics. However, the direct determination of uranium in bio-samples is challenging because of “ultra-low” concentrations of uranium, inherent matrix complexity, and sample diversity, which pose a great analytical challenge to existing detection methods. Here, we report on the direct, real-time, sensitive, and selective detection of uranyl ions in unprocessed and undiluted urine samples using a uranyl-binding aptamer-modified silicon nanowire-based field-effect transistor (SiNW-FET) biosensor, with a detection limit in the picomolar concentration range. The aptamer-modified SiNW-FET presented in this work enables the simple and sensitive detection of uranyl in urine samples. The experimental approach has a straight-forward implementation to other metals and toxic elements, given the availability of target-specific aptamers. Combining the high surface-to-volume ratio of SiNWs, the high affinity and selectivity of the uranyl-binding aptamer, and the distinctive sensing methodology gives rise to a practical platform, offering simple and straightforward sensing of uranyl levels in urine, suitable for field deployment and point-of-care applications.

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Human α-thrombin detection platform using aptamers on a silicon nanowire field-effect transistor

Cited by 3 publications

References 22 publications

Gating Hysteresis as an Indicator for Silicon Nanowire FET Biosensors

Gating Hysteresis as an Indicator for Silicon Nanowire FET Biosensors

Fabrication of Low Cost and Low Temperature Poly-Silicon Nanowire Sensor Arrays for Monolithic Three-Dimensional Integrated Circuits Applications

Direct Detection of Uranyl in Urine by Dissociation from Aptamer-Modified Nanosensor Arrays

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