Human X- and Y-bearing sperm differ in cell surface sialic acid content

Kaneko, Satoru; Oshio, Shigeru; Kobayashi, Toshifumi; Iizuka, R; Mohri, Hideo

doi:10.1016/0006-291x(84)91050-7

Cited by 62 publications

(42 citation statements)

References 6 publications

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“…Theoretically, however, the amount of H-Y antigen in X-and Y-sperm is considered to be similar. There is data [19,20] showing that human sperm can be successfully separated into two distinct populations by free-flow electrophoresis, which is based on differences in surface charge. However, there may be combined effects of surface charge and swimming characteristics when applied to separation of motile sperm.…”

Section: X-and Y-sperm Separationmentioning

confidence: 99%

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Hamano

2007

Journal of Reproduction and Development

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Abstract. Flow cytometrically-sorted sperm has been involved in the production of sex preselected offspring. More than 30,000 bovine offspring have been produced using AI and other means using spermatozoa separated by flow cytometer. Flow cytometric sperm sorting based on differences in their DNA content is the best method for separation of X-and Y-chromosome bearing spermatozoa. At first, flow cytometers were modified for DNA confirmation and sorting of sperm with high resolution. The beveled insertion needle can regulate orientation of flat-shaped bull sperm heads. The forward fluorescence detector is essential for measuring the DNA content of sperm. Recently, high-speed sperm sorting with orienting nozzles has resulted in production of 90% pure X-and Ysperm at rate of 15-20 million sperm per hour. Application of this new technique will enable conduct of more conventional technologies for both artificial insemination and cryopreservation in the bovine and in other farm animals using X-or Y-sperm.

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Section: X-and Y-sperm Separationmentioning

confidence: 99%

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Hamano

2007

Journal of Reproduction and Development

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“…Changes in protein composition in the membrane have been documented, for instance, a decrease in membrane P34H and an increase in alphatubulin after cryopreservation [20]. The mechanism involved in the zeta selection of sperm with strict normal morphology was based on the greater net negative charge of mature sperm due to membrane sialoglycoproteins [21,22], specifically, gp20-CD52 glycopolypeptides, acquired during transit through the epididymis [23]. Researchers have correlated the maturity of sperm membranes with strict normal morphology, an parameter indicative of successful fertilization [7,24,25].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, sperm selection by high-magnification motile sperm organelle morphology examination (MSOME) [29], electrophoresis [30] and hyaluronic acid hydrogel have recently been proposed [31]. While the zeta method has shortcomings such as low sperm recovery, a requirement of uncapacitated sperm [16,17] and the possible bias for X-bearing sperm [9,21,32,33], nevertheless, the zeta method appears promising for selecting thawed sperm for ICSI. However, more studies are needed before its application in routine ART treatment.…”

Section: Discussionmentioning

confidence: 99%

Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection

Kam

Jacobson

Patton

et al. 2007

J Assist Reprod Genet

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Purpose Mature sperm can be selected based on their negative zeta electrokinetic potential. The zeta selection of cryopreserved sperm is unknown. The objective was to study the effect of zeta processing on the morphology and kinematic parameters of cryopreserved-thawed sperm. Methods Colloid-washed sperm (N=9 cases) were cryopreserved for 24 h, thawed and diluted in serum-free medium in positive-charged tubes. After centrifugation, the tubes were decanted, serum-supplemented medium was added and the resuspended sperm were analyzed. Untreated sperm and fresh sperm served as the controls. Results There were improvements in strict normal morphology in fresh (11.8±0.3 versus control 8.8±0.3 %, mean ±SEM) and thawed (8.7±0.2 versus control 5.4±0.2%) sperm after zeta processing. Percent sperm necrosis was reduced after zeta processing (66.0±0.6 versus unprocessed 74.6±0.3%). Progression decreased by 50% but not total motility after zeta processing of thawed sperm. Conclusions The results suggested that the cryopreservation process did not impact the sperm membrane net zeta potential and higher percentages of sperm with normal strict morphology, acrosome integrity and reduced necrosis were recovered. The zeta method was simple and improved the selection of quality sperm after cryopreservation but more studies would be needed before routine clinical application.

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“…As diferenças fenotípicas tem sido alvo de estudo como: sensibilidade ao pH, carga elétrica de superfície de membrana espermática, morfologia do núcleo e cabeça, antígenos de superfície, velocidade de migração, gradiente de centrifugação e diferenças no conteúdo de DNA (30)(31)(32)(33)(34)(35)(36)(37)(38)(39).…”

Section: Métodos De Sexagem Espermáticaunclassified

Sêmen sexado através de citometria de fluxo e centrifugação por gradiente de concentração

et al. 2017

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ResumoO objetivo deste trabalho foi apresentar uma revisão completa e atualizada sobre a origem do sêmen sexuado, os requisitos e as técnicas de sexagem espermáticas. Nas últimas décadas, desenvolveram-se várias tecnologias na área da reprodução animal. O sêmen sexado é uma biotecnologia recente que ainda se encontra em fase de estudo e aperfeiçoamento em diversas etapas, devido a que o processo de sexagem causa estresse ao espermatozoide, e o deixa mais sensível ao processo de armazenamento; isto interfere diretamente na fertilidade. Esta tecnologia, que chegou ao Brasil em 2004, vem ganhando adeptos entre produtores que desejam produzir animais mais homogêneos para o sacrifí-cio ou para a produção de leite. O uso do sêmen sexado tem sido difundido em muitos rebanhos do mundo, já que se apresenta como uma técnica com um direcionamento maior na seleção do sexo. Muitas técnicas têm sido utilizadas com a finalidade de buscar uma maior separação de espermatozoides portadores de cromossomas X e Y, com mínima agressão espermática. Desta forma, a utilização da sexagem espermática tem obtido resultados na aplicação de biotecnologias da reprodução assistida, e com isso tem elevado o lucro no melhoramento genético e tem otimizado a produtividade no sexo escolhido. Entretanto, ainda existem muitas limitações, principalmente referentes al tempo de exposição, o índice de aproveitamento e os resultados de índices de gestação em condições de campo. Por outro lado, a dose de sêmen sexado pode custar de duas a oito vezes o valor de uma convencional; da mesma forma, o seu uso avança no Brasil.Palavras chave: bovinos, biotecnologia, sexagem espermática, cromossomas. Semen sexed using flow cytometry and concentration gradient centrifugation AbstractThis study aimed to present a complete and updated review on the origin of sexed semen, as well as on the requirements and techniques of sperm sexing. In the last decades, several technologies were developed in the field of animal reproduction. Sexed semen is a recent biotechnology that is still under study and refinement in various stages, because the sexing process causes stress to the sperm, leaving it more sensitive to the storage process; this directly interferes with fertility. This technology, which arrived in Brazil in 2004, has been gaining support among producers who want to produce more homogeneous animals for slaughter or for dairy production. The use of sexed semen has been disseminated in many herds of the world, since it is a technique with better results in the selection of sex. Many techniques have been used in order to seek a greater separation of sperms carrying X and Y chromosomes, with minimal sperm aggression. In this way, the use of sperm sexing has obtained results in the application of assisted reproductive bio-

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Human X- and Y-bearing sperm differ in cell surface sialic acid content

Cited by 62 publications

References 6 publications

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection

Sêmen sexado através de citometria de fluxo e centrifugação por gradiente de concentração

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