Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Hamano, Koh‐ichi

doi:10.1262/jrd.18141

Cited by 21 publications

(18 citation statements)

References 68 publications

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“…The percentage of X sperm using qPCR was 89%, which was within the expected 85% to 95% obtained by flow cytometry (Cran, 2007;Hamano, 2007;Seidel Jr., 2009). …”

Section: Discussionsupporting

confidence: 75%

Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient

Resende

Lucio

Perini

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient [Validação comparativa utilizando PCR quantitativo em tempo real (qPCR) ABSTRACTThe objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P<0.05). The percentage of female embryos in the Percoll and OptiPrep groups was 62.0% and 47.1%, respectively. These results were confirmed by qPCR of spermatozoa DNA and underestimation was seen only in the Percoll group. It was possible to sexing sperm using simple approach.

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“…The percentage of X sperm using qPCR was 89%, which was within the expected 85% to 95% obtained by flow cytometry (Cran, 2007;Hamano, 2007;Seidel Jr., 2009). …”

Section: Discussionsupporting

confidence: 75%

Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient

Resende

Lucio

Perini

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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“…The need for producing replacement heifers in commercial dairy herds has led to development of several methods for separating X and Y bearing chromosomes, in the recent decades. Among the proposed sex pre-selection methods, flowcytometery has been relatively successful in commercial production of sex sorted semen (Hamano 2007;Seidel 2007). This assisted reproductive technology acts based on the inherent difference in DNA content of X and Y bearing sperms.…”

Section: Introductionmentioning

confidence: 99%

Reproductive performance of sexed versus conventional semen in Holstein heifers in various semiarid regions of Iran

Joezy-Shekalgorabi

Maghsoudi

Mansourian³

2017

Italian Journal of Animal Science

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For comparing reproductive performance of sexed versus conventional semen a total of 3573 heifer insemination records collected from five herds in four provinces of Iran were investigated. The studied provinces were classified into three regions: hot semiarid (Tehran and Alborz provinces), temperate semiarid (Khorasan Razavi province) and cold semiarid (Zanjan province). Various parameters including the conception rate, number of services per conception, calf sex ratio, calf birth weight, gestation length, calving ease score, abortion and stillbirth as well as twining rate were investigated. The logistic regression method was deployed for the analysis of categorical variables while the GLM procedure was applied for the analysis of continuous variables. The average conception rate of sex sorted and conventional semen was 48.3 and 63.8%, respectively. The utilisation of sex sorted semen resulted in 91.1% female calves. The conception rate, number of services per conception, gestation length, calf birth weight and the calf sex ratio were among the reproductive variables that were significantly influenced by the semen sexing. A greater number of services per conception and calving ease score were observed in hot and temperate semiarid areas. These climatic regions also provided a lower incidence of female calf births compared to the cold semiarid regions. Our results highly reaffirm the previous findings on the reproductive performance of sex sorted semen. Yet, climatic and management practices of a herd within a special climate have to be considered when deciding for utilisation of sex sorted semen in a dairy farm. ARTICLE HISTORY

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“…Currently, by applying flow cytometer, analysis rates of about 20,000 sperm per second and a sorting speed of up to 6000 or more spermatozoa per second, each of X and Y populations, reaching purities around 90% or better, have been described. Under these conditions, approximately 15 -20 million sorted X and Y spermatozoa could be produced per hour in most farm animal species (Hamano, 2007). The following are some of the factors that affect the sorting rate and efficiency.…”

Section: Type Of Sperm Sorter and Sorting Efficiencymentioning

confidence: 99%

Sexing of Spermatozoa in Farm Animals: a Mini Review

Prakash¹

2014

Adv. Anim. Vet. Sci.

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Livestock farmers always have a wish for producing young ones of desired sex. Among the several techniques available, use of sexed semen for artificial insemination is recognized as more pragmatic and easy way to pre -select the sex of the offspring. Selective use of sexed semen in breeding will not only increase the genetic progress from the daughter -dam path but would also help in producing good male germplasm from elite bulls for future breeding. Several attempts have been made, elsewhere in the globe, to develop methods that efficiently separate bovine semen into fractions containing higher concentrations of X -or Y -bearing sperm. These technologies include sex specific antibodies, centrifugation and flow cytometry. Of these attempts, the only method proven to be commercially viable is flow cytometry. However, sorting pressure, speed, electrical deviation, laser radiation all leads to membrane alteration and pre -capacitation like changes in the sorted sperm leading to reduced fertility. Despite these limitations, production of sexed semen usually followed by cryopreservation is being used commercially for cattle production. Development of the instrument for increasing the sorting rate and also purity of sorting without affecting the viability and fertility is still an active area of research. The aim of this review is to update the readers with the recent developments in sexing of spermatozoa with special reference to farm animals.All copyrights reserved to Nexus® academic publishers

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Sex Preselection in Bovine by Separation of X- and Y-Chromosome Bearing Spermatozoa

Cited by 21 publications

References 68 publications

Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient

Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient

Reproductive performance of sexed versus conventional semen in Holstein heifers in various semiarid regions of Iran

Sexing of Spermatozoa in Farm Animals: a Mini Review

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