Human Urine Proteome: A Powerful Source for Clinical Research

Zou, Lili; Sun, Wei

doi:10.1007/978-94-017-9523-4_4

Cited by 32 publications

(26 citation statements)

References 48 publications

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“…Normal urine contains a small but constant amount of proteins, derived from each nephron segment, but also from blood circulation. In fact, low MW and cationic proteins can be filtered and are detectable by proteomic analysis [19]. In fact, proteinuria is now being considered a quantitative process.…”

Section: Urinomementioning

confidence: 99%

The human urinary exosome as a potential metabolic effector cargo

Bruschi

Ravera

Santucci

et al. 2015

Expert Review of Proteomics

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Exosomes are nanovesicles, derived from the endocytic pathway, released by most cell types and found in many body fluids, including urine. A variety of exosomal functions have been reported, including transfer of RNA, cell communication, control of apoptosis and protein lifespan. Exosomes from mesenchymal stem cells can rescue bioenergetics of injured cells. Here the urinary exosome proteome, non-urinary exosome proteome and urinome are compared. A consistent number of identified proteins cluster to metabolic functions. Cytoscape software analysis based on biological processes gene ontology database shows that metabolic pathways such as aerobic glycolysis and oxidative phosphorylation have a high probability (p ≤ 0.05) of being expressed and therefore functional. A metabolic function appears to be associated with human urinary exosomes, whose relevance experimental studies can assess.

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Section: Urinomementioning

confidence: 99%

The human urinary exosome as a potential metabolic effector cargo

Bruschi

Ravera

Santucci

et al. 2015

Expert Review of Proteomics

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“…Urine definitely is not a waste in regards to gaining patients’ diagnostics and therapeutic information [ 18 , 44 , 45 ]. However, it is still in debate whether urine plays an active role in regulating bladder biology.…”

Section: Discussionmentioning

confidence: 99%

“…Proteomes are directly responsible for cell functions, and therefore, abnormal protein expression is an indication of cellular disruption due to the pathological conditions [ 14 , 15 ]. Current global proteomic technologies may provide a comprehensive understanding of urological diseases, characteristics of the disease’s state, and novel approaches to relieve the clinical symptoms [ 16 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

Urinary proteomics and metabolomics studies to monitor bladder health and urological diseases

Chen

Kim

2016

BMC Urol

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BackgroundAssays of molecular biomarkers in urine are non-invasive compared to other body fluids and can be easily repeated. Based on the hypothesis that the secreted markers from the diseased organs may locally release into the body fluid in the vicinity of the injury, urine-based assays have been considered beneficial to monitoring bladder health and urological diseases. The urine proteome is much less complex than the serum and tissues, but nevertheless can contain biomarkers for diagnosis and prognosis of diseases. The urine metabolome has a much higher number and concentration of low-molecular metabolites than the serum or tissues, with a far lower lipid concentration, yet informs directly about dietary and microbial metabolism.DiscussionWe here discuss the use of mass spectrometry-based proteomics and metabolomics for urine biomarker assays, specifically with respect to the underlying mechanisms that trigger the pathological condition.ConclusionMolecular biomarker profiles, based on proteomics and metabolomics studies, reliably distinguish patients from healthy controls, stratify sub-populations with respect to treatment options, and predict therapeutic response of patients with urological disease.

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“…На сегодняшний день реализуется другой не менее масштабный проект -изучение про теома человека. Параллельно с исследовани ем плазмы крови исследуется белковый со став и других биологических жидкостей, на пример мочи [3,4,9,24], слезы [5,18], cлюны [6,10,23], эякулята [1] и т.д. [11,12,13].…”

Section: Introductionunclassified

Urinary and blood procalcitonin concentration in healthy persons

Sosnin

Юрьевич²,

Nenasheva

et al. 2019

Perm Med J

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Aim. Blood serum and urinary procalcitonin (PCT) concentration in healthy persons was studied. Materials and methods. A single-stage observational study of case-control type was performed. The study included 32 men and 37 women of middle age (53.4 16.4 years) with normal renal function. PTC concentration was determined with the method of solid-phase enzyme immunoassay using test-system (Procalcitonin IFA-BEST, Russia). Results. Blood serum PCT concentration in the examined persons was 0.029 0.016 ng/ml (M SD). The number of blood serum samples with PTC level 0.05 ng/ml was 5.8 % (4 from 69). The mean urinary PTC concentration by 72.59 times exceeded the mean blood serum PTC content and was 2.12 1.832 ng/ml (р 0.000001). Coefficient of variation of results for the urine by 1.57 times exceeded the analogous index for the blood serum. When comparing the results of blood serum and urine analyses, no statistically significant differences between men and women were revealed. When estimating the coefficients of linear correlation between PTC content in the blood serum and urine, a weak positive dependence was established (R = 0.302782). Conclusions. High PTC concentration in the urine permits to suppose that one of the ways of procalcitonin removal from the blood plasma is its elimination by kidneys in unchanged type by means of glomerular filtration.

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Human Urine Proteome: A Powerful Source for Clinical Research

Cited by 32 publications

References 48 publications

The human urinary exosome as a potential metabolic effector cargo

The human urinary exosome as a potential metabolic effector cargo

Urinary proteomics and metabolomics studies to monitor bladder health and urological diseases

Urinary and blood procalcitonin concentration in healthy persons

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