Aim. To study the dynamics of monocyte chemotactic factor (MCP-1) content in the ejaculate of healthy men and men with diminished spermatozoid concentration. Material and methods.Sixty-four men were examined. The main group included 16 patients with azoospermia. The comparison group – 24 patients with oligozooasthenospermia (spermatozoid concentration lower than 15 mln/ml). The control group was presented by ejaculate samples of 24 healthy men, characterized by normal parameters. The MCP-1 concentration was determined with the method of solid phase enzyme immunoassay using the assay kit “MCP-1-EIA-BEST” (A8784) (CJSC Vector-Best, Russia). Results. The mean MCP-1 concentration in the samples of undiluted seminal plasma was 2242.8 ± 672.0 pg/ml versus the blood serum, where MCP-1 content was 18.9 times lower and was 118.8 ± 22.9 pg/ml. No reliable differences were revealed when comparing the mean values of MCP-1 in the studied groups in ejaculates; there were also no statistically significant difference between the indices in MCP-1 groups in the blood serum. Conclusions. Male seminal plasma is characterized by unusually high MCP-1 content, exceeding more than tenfold the concentration of this protein in the blood serum. High MCP-1 concentration in sperm and the absence of its dependence on the concentration of this protein in blood serum indicates local production of this protein into the seminal plasma by male reproductive organs. Further studies are needed to find out a concrete localization of the sites of production of this protein in male genital organs and to study its probable role in reproductive processes in male and female organisms.
___________________________________________________________________________________Цель. Изучить зависимость антиоксидантного статуса (АОС) от возраста, пола, локализации и разме ров очага повреждения у больных в острейшем периоде развития атеротромботического варианта ишемического инсульта. Материалы и методы. Обследовано 74 пациента (основная группа) в острейшем периоде атеро тромботического варианта ишемического инсульта. Группу сравнения, сопоставимую по возрасту и полу, составили 15 здоровых лиц. Общий антиоксидантный статус (ОАС) сыворотки крови исследовали с ис пользованием наборов реактивов компании «Вектор -Бест» В 7501 (ЗАО «Вектор Бест», Россия). Результаты. Значение АОС в группе сравнения составило 1,69 ± 0,17 ммоль/л (медиана 1,72; интер квартильный размах 1,53 -1,83 ммоль/л), что соответствовало интервалу нормы, указанному в инст рукции к набору реактивов (1,3-1,8 ммоль/л). Результаты ОАС не различались у мужчин и женщин (крите рий U = 26,0; p = 0,816). У пациентов основной группы АОС был снижен (р = 0,000034). Величина АОС со ставила 1,14 ± 0,4 ммоль/л (медиана 1,12 ммоль/л, интерквартильный размах 0,81 -1,45 ммоль/л). Пол, возраст и локализация очага инсульта достоверно не влияли на степень снижения АОС. Установлена умеренная обратная корреляция между размерами очага и снижением значения АОС. Коэффициент ранговой корреляции Спирмена составил -0,79 (p < 0,001). Выводы. Снижение ОАС в острейшем периоде развития атеротромботического инсульта не зависит от пола, возраста, локализации ишемического очага, но зависит от размеров очага, подвергшегося ишемизации. Ключевые слова. Ишемический инсульт, свободные радикалы, антиоксиданты. Aim.To study the dependence of antioxidant status (AOS) on the age, sex, localization and size of the damage focus in patients during the most acute period of the development of atherothrombotic variant of ischemic stroke. Materials and methods. Seventy four patients (main group) in the most acute period of atherothrombotic variant of ischemic stroke were examined. The group of comparison, comparable by age and sex, included
Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy
Aim. Blood serum and urinary procalcitonin (PCT) concentration in healthy persons was studied. Materials and methods. A single-stage observational study of case-control type was performed. The study included 32 men and 37 women of middle age (53.4 16.4 years) with normal renal function. PTC concentration was determined with the method of solid-phase enzyme immunoassay using test-system (Procalcitonin IFA-BEST, Russia). Results. Blood serum PCT concentration in the examined persons was 0.029 0.016 ng/ml (M SD). The number of blood serum samples with PTC level 0.05 ng/ml was 5.8 % (4 from 69). The mean urinary PTC concentration by 72.59 times exceeded the mean blood serum PTC content and was 2.12 1.832 ng/ml (р 0.000001). Coefficient of variation of results for the urine by 1.57 times exceeded the analogous index for the blood serum. When comparing the results of blood serum and urine analyses, no statistically significant differences between men and women were revealed. When estimating the coefficients of linear correlation between PTC content in the blood serum and urine, a weak positive dependence was established (R = 0.302782). Conclusions. High PTC concentration in the urine permits to suppose that one of the ways of procalcitonin removal from the blood plasma is its elimination by kidneys in unchanged type by means of glomerular filtration.
Objective. The aim of the study was to investigate the effectiveness of the preparation of thioctic acid combined with standard therapy among patients with angiodistonic syndrome and vegetative-sensory polyneuropathy of the extremities due to the influence of general vibration. Materials and methods. A prospective randomized study of two groups of patients with vibration disease was conducted: the main group (n = 30) and the comparison group (n = 30). All patients received standard therapy, while the main group received additional therapy with octolipen (300 mg per os 2 times a day). Results. The results of questionnaires and objective examination of patients did not establish the occurrence of any side effects when including the drug in the therapy. The survey data proved statistically significant differences in the assessment of pain syndrome at rest and in the occurrence of night pains (p = 0.022). At the same time, when assessing the severity of paresthesia, no statistically significant difference was found between the groups (p = 0.5536). The therapy was accompanied by normalization of the antioxidant status (AOS) in both groups: in the main group, the median AOS increased by 1.342 times and in the comparison group by 1.267 times. In the statistical evaluation, the inclusion of thioctic acid in the treatment was accompanied by a statistically significant increase in the AOS not only with the initial data (p 0.00001), but also in comparison with the results of treatment of patients in the comparison group (p = 0.0387). The data from the reovasographic study also showed that the therapy was more effective with additional inclusion of thioctic acid drug to it. Conclusions. Thus, the data obtained justify the feasibility and effectiveness of using thioctic acid preparation in the treatment of angiodistonia and polyneuropathy syndromes.
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