Human skin penetration of gold nanoparticles through intact and damaged skin

Filon, Francesca Larese; Crosera, Matteo; Adami, Gianpiero; Bovenzi, Massimo; Rossi, Federica; Maina, Giovanni

doi:10.3109/17435390.2010.551428

Cited by 107 publications

(45 citation statements)

References 47 publications

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“…A recent report by Larese Filon et al (2011) supports the earlier findings of Sonavane. They found similar results when evaluating gold nanoparticle penetration (12.9 nm) in thawed human skin (29). Gold nanoparticles were detected in the receiving solution of the Franz cells after 24 h of skin exposure.…”

Section: Introductionsupporting

confidence: 53%

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Gold Nanoparticle Penetration and Reduced Metabolism in Human Skin by Toluene

et al. 2011

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Section: Introductionsupporting

confidence: 53%

“…These data were gathered from a depth series of en face images. 24 h using ICP to detect gold ions in the receptor (29). Our study showed penetration into stratum corneum but not beyond using 14.9-nm gold nanoparticles in aqueous solution with TEM, MPT-FLIM, and RCM.…”

Section: Discussionmentioning

confidence: 70%

Gold Nanoparticle Penetration and Reduced Metabolism in Human Skin by Toluene

et al. 2011

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“…The same author also reported the effect of surface modifications, size, vehicles, and concentrations of AuNPs over human skin permeation19. Larese et al 20. performed permeation experiments on intact and damaged human skin and reported that the AuNPs penetrated deeper in the layer and flux of AuNPs for damaged skin was an order of higher than that of intact skin.…”

mentioning

confidence: 98%

Effect of Size and Surface Charge of Gold Nanoparticles on their Skin Permeability: A Molecular Dynamics Study

Gupta

Rai

2017

Sci Rep

176

138

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Molecular level understanding of permeation of nanoparticles through human skin establishes the basis for development of novel transdermal drug delivery systems and design and formulation of cosmetics. Recent experiments suggest that surface coated nano-sized gold nanoparticles (AuNPs) can penetrate the rat and human skin. However, the mechanisms by which these AuNPs penetrate are not well understood. In this study, we have carried out coarse grained molecular dynamics simulations to explore the permeation of dodecanethiol coated neutral hydrophobic AuNPs of different sizes (2–5 nm) and surface charges (cationic and anionic) through the model skin lipid membrane. The results indicate that the neutral hydrophobic AuNPs disrupted the bilayer and entered in it with in ~200 ns, while charged AuNPs were adsorbed on the bilayer headgroup. The permeation free energy calculation revealed that at the head group of the bilayer, a very small barrier existed for neutral hydrophobic AuNP while a free energy minimum was observed for charged AuNPs. The permeability was maximum for neutral 2 nm gold nanoparticle (AuNP) and minimum for 3 nm cationic AuNP. The obtained results are aligned with recent experimental findings. This study would be helpful in designing customized nanoparticles for cosmetic and transdermal drug delivery application.

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“…Excised human skin is often stored for a period of 24 hours to several weeks under varying temperatures ranging from 4°C to −80°C before it is used for NP penetration studies by different groups. It is important to note that skin handling, storage and processing parameters may alter the results obtained [11,16,59,60]. Pentland et al, demonstrated that skin trauma induced during subcutaneous fat removal causes mast cell degranulation and increases tissue vascularity [42].…”

Section: Resultsmentioning

confidence: 99%

Impact of Cosmetic Lotions on Nanoparticle Penetration through ex Vivo C57BL/6 Hairless Mouse and Human Skin: A Comparison Study

et al. 2016

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Understanding the interactions of nanoparticles (NPs) with skin is important from a consumer and occupational health and safety perspective, as well as for the design of effective NP-based transdermal therapeutics. Despite intense efforts to elucidate the conditions that permit NP penetration, there remains a lack of translatable results from animal models to human skin. The objectives of this study are to investigate the impact of common skin lotions on NP penetration and to quantify penetration differences of quantum dot (QD) NPs between freshly excised human and mouse skin. QDs were mixed in 7 different vehicles, including 5 commercial skin lotions. These were topically applied to skin using two exposure methods; a petri dish protocol and a Franz diffusion cell protocol. QD presence in the skin was quantified using Confocal Laser Scanning Microscopy. Results show that the commercial vehicles can significantly impact QD penetration in both mouse and human skin. Lotions that contain alpha hydroxyl acids (AHA) facilitated NP penetration. Lower QD signal was observed in skin studied using a Franz cell. Freshly excised human skin was also studied immediately after the sub-cutaneous fat removal process, then after 24 hours rest ex vivo. Resting human skin 24 hours prior to QD exposure significantly reduced epidermal presence. This study exemplifies how application vehicles, skin processing and the exposure protocol can affect QD penetration results and the conclusions that maybe drawn between skin models.

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Human skin penetration of gold nanoparticles through intact and damaged skin

Cited by 107 publications

References 47 publications

Gold Nanoparticle Penetration and Reduced Metabolism in Human Skin by Toluene

Gold Nanoparticle Penetration and Reduced Metabolism in Human Skin by Toluene

Effect of Size and Surface Charge of Gold Nanoparticles on their Skin Permeability: A Molecular Dynamics Study

Impact of Cosmetic Lotions on Nanoparticle Penetration through ex Vivo C57BL/6 Hairless Mouse and Human Skin: A Comparison Study

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