Gold Nanoparticle Penetration and Reduced Metabolism in Human Skin by Toluene

Labouta, Hagar I.; Liu, David C.; Lin, Lynlee L.; Butler, Margaret K.; Grice, Jeffrey E.; Raphael, Anthony P.; Kraus, Tobias; El-Khordagui, Labiba K.; Soyer, H. Peter; Roberts, Michael S.; Schneider, Marc; Prow, Tarl W.

doi:10.1007/s11095-011-0561-z

Cited by 85 publications

(69 citation statements)

References 42 publications

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“…Two types of AuNPs were tested in the presence of keratinocytes for possible toxicity and to select the non or less nanocarrier toxicity [69]. As shown in Fig.…”

Section: Morphology Of Keratinocytes After Au-3mps and Au-dea Treatmentmentioning

confidence: 99%

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Bessar

Venditti

Benassi

et al. 2016

Colloids and Surfaces B: Biointerfaces

113

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a b s t r a c tGold nanoparticles (AuNPs) represent an effective choice for topical drug delivery systems thanks to their small size, general non-toxicity, ease of functionalization and high surface to volume ratio. Even if systemic, methotrexate still plays an important role in psoriasis treatment: its topical use shows insufficient percutaneus penetration owing to limited passive diffusion, high molecular weight and dissociation at physiological pH. The aim of our study was to design a new drug delivery nanocarrier for Methotrexate and to improve its solubility, stability and biodistribution. AuNPs were on purpose prepared with a hydrophilic stabilizing layer, in order to improve the colloidal stability in water. Watersoluble gold nanoparticles functionalized by sodium 3-mercapto-1-propansulfonate (Au-3MPS) were prepared and loaded with methotrexate (MTX). The loading efficiency of MTX on Au-3MPS was assessed in the range 70-80%, with a fast release (80% in one hour). The release was studied up to 24 h reaching the value of 95%. The Au-3MPS@MTX conjugate was fully characterized by spectroscopic techniques (UV-vis, FTIR) and DLS. Preliminary toxicity tests in the presence of keratinocytes monolayers allowed to assess that the used Au-3MPS are not toxic. The conjugate was then topically used on C57BL/6 mouse normal skin in order to trace the absorption behavior. STEM images clearly revealed the distribution of gold nanoparticles inside the cells. In vitro studies showed that Methotrexate conjugated with Au-3MPS is much more efficient than Methotrexate alone. Moreover, DL50, based on MTT analysis, is 20 folds reduced at 48 h, by the presence of nanoparticles conjugation. UV-vis spectra for in vivo tracing of the conjugate on bare mouse skin after 24 h of application, show increased delivery of Methotrexate in the epidermis and dermis using Au-3MPS@MTX conjugate, compared to MTX alone. Moreover we observed absence of the Au-3MPS in the dermis and in the epidermis, suggesting that these layers of the skin do not retain the nanoparticles. Based on our data, we found that the novel Au-3MPS@MTX conjugate is an effective non-toxic carrier for the satisfactory percutaneous absorption of Methotrexate and could help in possible topical treatment of psoriasis.

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“…Two types of AuNPs were tested in the presence of keratinocytes for possible toxicity and to select the non or less nanocarrier toxicity [69]. As shown in Fig.…”

Section: Morphology Of Keratinocytes After Au-3mps and Au-dea Treatmentmentioning

confidence: 99%

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Bessar

Venditti

Benassi

et al. 2016

Colloids and Surfaces B: Biointerfaces

113

View full text Add to dashboard Cite

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“…Localization of GNS-PEG and GNS-PEG-OAm in subcutaneous adipose tissues was further supported by TEM, which has been previously used to access the extent of nanoparticle deposition in skin layers. 40,41 TEM also revealed that the permeated nanoparticles were well dispersed inside the subcutaneous adipose tissue and eliminated the concerns of fluorescence quenching.…”

Section: In Vivo Skin Permeation Assessmentmentioning

confidence: 90%

Enhancing the in vivo transdermal delivery of gold nanoparticles using poly(ethylene glycol) and its oleylamine conjugate

Hsiao

Peng

Tang

et al. 2016

IJN

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In this study, we investigated the effect of (ethylene glycol) (PEG) and PEG–oleylamine (OAm) functionalization on the skin permeation property of gold nanoparticles (GNS) in vivo. Chemisorption of polymers onto GNS was verified by a red shift in the ultraviolet–visible spectrum as well as by a change in the nanoparticle surface charge. The physicochemical properties of pristine and functionalized nanoparticles were analyzed by ultraviolet–visible spectroscopy, zeta potential analyzer, and transmission electron microscopy. Transmission electron microscopy revealed that the interparticle distance between nanoparticles increased after GNS functionalization. Comparing the skin permeation profile of pristine and functionalized GNS, the follicular deposition of GNS increased twofold after PEG–OAm functionalization. Moreover, PEG- and PEG–OAm-functionalized nanoparticles were able to overcome the skin barrier and deposit in the deeper subcutaneous adipose tissue. These findings demonstrate the potential of PEG- and PEG–OAm-functionalized GNS in serving a multitude of applications in transdermal pharmaceuticals.

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“…[17] badali wnikanie w skórę kulistych cząstek o charakterze hydrofilowym lub hydrofobowym o średnicy 6 nm lub 15 nm, w wycinkach skóry ludzkiej od pacjentów po abdominoplastyce. Badacze wykazali większą penetrację nanocząstek o średni-cy 6 nm w porównaniu z cząstkami wielkości 15 nm.…”

Section: Przenikanie Nanocząstek Złota Przez Skóręunclassified

Nanogold – Biological effects and occupational exposure levels

Świdwińska-Gajewska

Czerczak

2017

Med Pr

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StreszczenieNanozłoto różni się właściwościami i działaniem biologicznym od złota metalicznego. Może ono znaleźć zastosowanie w wielu dziedzinach, takich jak medycyna, diagnostyka laboratoryjna czy elektronika. Z badań przeprowadzonych na zwierzętach laboratoryjnych wynika, że nanozłoto może się wchłaniać drogą oddechową i pokarmową. Może penetrować w głąb naskórka i skóry właściwej, ale nie ma dowodów, że wchłania się przez skórę. Nanoobiekty złota kumulują się głównie w wątrobie i śledzionie, ale mogą docierać do innych narządów wewnętrznych. Nanozłoto może pokonywać bariery krew-mózg i krew-łożysko. Toksykokinetyka nanozłota zależy od wielkości cząstek, kształtu oraz ładunku powierzchniowego. U zwierząt narażanych drogą inhalacyjną nanocząstki złota wywoływały niewielkie zmiany w płucach. Podawane drogą pokarmową nie powodowały negatywnych skutków zdrowotnych u gryzoni. U zwierząt, którym wstrzykiwano dootrzewnowo nanoobiekty złota, obserwowano zmiany w wątrobie i płucach. Wykazano genotoksyczność nanozłota w badaniach in vitro na komórkach, ale nie potwierdzono takiego działania u zwierząt. Nie zaobserwowano szkodliwego wpływu nanoobiektów na płód czy rozrodczość. Nie ma badań dotyczą-cych działania rakotwórczego nanocząstek złota. Mechanizm działania toksycznego nanozłota może być związany z jego oddziaływaniem z białkami i DNA, co w efekcie prowadzi do indukowania stresu oksydacyjnego lub uszkodzeń materiału genetycznego. Wpływ nanostruktur na zdrowie człowieka nie jest jeszcze w pełni wyjaśniony. Osoby pracujące z nanomateriałami powinny zachować szczególną ostrożność i stosować istniejące zalecenia przy ocenie narażenia zawodowego na nanoobiekty. Przeprowadzona ocena ryzyka powinna stanowić podstawę do podejmowania odpowiednich działań ograniczających potencjalne narażenie na nanometale, w tym również nanozłoto. Med. Pr. 2017;68(4):545-556 Słowa kluczowe: nanocząstki, narażenie zawodowe, nanoobiekty, toksyczność, nanozłoto, toksykokinetyka AbstractNanogold has different properties and biological activity compared to metallic gold. It can be applied in many fields, such as medicine, laboratory diagnostics and electronics. Studies on laboratory animals show that nanogold can be absorbed by inhalation and ingestion. It can penetrate deep into the epidermis and dermis, but there is no evidence that it is absorbed through the skin. Gold nanoobjects accumulate mainly in the liver and spleen, but they can also reach other internal organs. Nanogold can cross the blood-brain and blood-placenta barriers. Toxicokinetics of nanogold depends on the particle size, shape and surface charge. In animals exposure to gold nanoparticles via inhalation induces slight changes in the lungs. Exposure to nanogold by the oral route does not cause adverse health effects in rodents. In animals after injection of gold nanoobjects changes in the liver and lungs were observed. Nanogold induced genotoxic effects in cells, but not in animals. No adverse effects on the fetus or reproduction were found. There are no carcinogenicity studie...

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Gold Nanoparticle Penetration and Reduced Metabolism in Human Skin by Toluene

Cited by 85 publications

References 42 publications

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Enhancing the in vivo transdermal delivery of gold nanoparticles using poly(ethylene glycol) and its oleylamine conjugate

Nanogold – Biological effects and occupational exposure levels

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