Human Serum is as Efficient as Fetal Bovine Serum in Supporting Proliferation and Differentiation of Human Multipotent Stromal (Mesenchymal) Stem Cells In Vitro and In Vivo

Aldahmash, Abdullah; Haack‐Sørensen, Mandana; Al-Nbaheen, May; Harkness, Linda; Abdallah, Basem M.; Kassem, Moustapha

doi:10.1007/s12015-011-9274-2

Cited by 72 publications

(68 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Human Serum (HS): Overall, HS allowed for the isolation and expansion of BMSC, ASC, DPSC maintaining proper cell biological properties both in vitro and in vivo (48,(80)(81)(82)(83)(84)(85). Both, 10% HS in DMEM/F-12 or 5%-10% HS in αMEM provided, to ASC and BMMSC, proliferation rates and multipotency as higher as 10% FBS (24,48,51,(86)(87)(88).…”

Section: Resultsmentioning

confidence: 99%

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

et al. 2017

View full text Add to dashboard Cite

Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS). FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can unleash antigenic responses in patients after MSC implantation. A wide range of venous blood derivatives (VBD) has been reported as FBS substitutes showing promising results. Thus, the aim of this study was to conduct a systematic scoping review to analyze whether VBD are effective FBS substitutes for MSC ex vivo expansion. The search was performed in SciVerse Scopus TM , PubMed, Web of Science TM , BIREME, Cochrane library up to January 2016. The keywords were selected using MeSH and entry terms. Two independent reviewers scrutinized the records obtained considering specific inclusion criteria. The included studies were evaluated in accordance with a modified Arksey and O' Malley's framework. From 184 found studies, 90 were included. Bone marrow mesenchymal stem cells (BMMSC) were presented in most of these studies. Overall, VBD allowed for either, maintenance of MCS's fibroblast-like morphology, high proliferation, high colony-formation ability and maintenance of multipotency. Besides. MSC expanded in VBD supplements presented higher mitogen activity than FBS. VBD seems to be excellent xeno-free serum for ex vivo expansion of mesenchymal stem cells. However, an accentuated heterogeneity was observed between the carried out protocols for VBD isolation did not allowing for direct comparisons between the included studies.

show abstract

Section: Resultsmentioning

confidence: 99%

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Scale bar =200 µm. (Aldahmash et al, 2011;Wang et al, 2012). Previously we have observed that human eyelid adiposederived stem cells could aggregate during high-density culture in the presence of HS (Song et al, 2012).…”

Section: Protein Expression Of Jam2 Moleculementioning

confidence: 96%

Expression of Tight Junction Molecule In The Human Serum-Induced Aggregation of Human Abdominal Adipose-Derived Stem Cells In Vitro

Yoon¹,

Yun²,

Yang³

et al. 2014

Development &Amp; Reproduciton

View full text Add to dashboard Cite

“…Among the blood-derived components, human AB serum (AB HS) can be considered a promising FBS alternative due to its availability and possibility of autologous usage [6]. Various studies have reported on the successful use of this supplement for different types of cells, including MSCs [4,[6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Various studies have reported on the successful use of this supplement for different types of cells, including MSCs [4,[6][7][8][9][10][11]. However, none of these studies have addressed different sources to produce AB HS or con-…”

Section: Introductionmentioning

confidence: 99%

Characterization of Human AB Serum for Mesenchymal Stromal Cell Expansion

Santos

Mizukami

Orellana

et al. 2016

Transfus Med Hemother

View full text Add to dashboard Cite

Background: So far, using human blood-derived components appears to be the most efficient and safest approach available for mesenchymal stromal cell (MSC) expansion. In this paper, we report on the characterization of human AB serum (AB HS) produced by using different plasma sources, and its use as an alternative supplement to MSC expansion. Methods: Two plasma sources were used for AB HS production: plasma removed from whole blood after 24 h of collection (PC > 24 h) and plasma, cryoprecipitate reduced (PCryoR). The biochemical profile and quality of the produced AB HS batches were analyzed and their ability to support MSC cell growth after different storage times (0, 3, 6, 9 and 12 months) was evaluated. Results: The two plasma sources used showed similar characteristics regarding biochemical constituents and quality parameters and were effective in promoting MSC growth. MSCs cultured in medium supplemented with 10% AB HS presented similar doubling times and cumulative population doublings when compared to the 10% fetal bovine serum(FBS)-supplemented culture while maintaining immunophenotype, functional features, and cytogenetic profile. Conclusion: Overall, the results indicate that AB HS is an efficient FBS substitute and can be used for at least 12 months after production without impairing cell proliferation and quality.

show abstract

Human Serum is as Efficient as Fetal Bovine Serum in Supporting Proliferation and Differentiation of Human Multipotent Stromal (Mesenchymal) Stem Cells In Vitro and In Vivo

Abstract: Human serum can support proliferation and differentiation of hMSC in vitro and can maintain their bone forming capacity in vivo. The use of human serum in cell cultures of hMSC intended for cell-based therapy is preferable.

Cited by 72 publications

References 31 publications

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

Expression of Tight Junction Molecule In The Human Serum-Induced Aggregation of Human Abdominal Adipose-Derived Stem Cells In Vitro

Characterization of Human AB Serum for Mesenchymal Stromal Cell Expansion

Contact Info

Product

Resources

About