Human Papillomavirus Infection in the Oral Cavity of Denture Wearers

Nishimura, Yoshie; Maeda, Hideaki; Hattori, Masami; Azumaya, Fumihiro; Muramatsu, Ikuro; Kameyama, Yôichiro; Tanaka, Yoshinobu; Kawaguchi, Toyozo

doi:10.2186/jjps.48.713

Cited by 13 publications

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“…Considering these characteristic of HPV infection, it can be assumed that HPV can easily invade and infect cells of OLP lesions where the oral mucosa is often damaged due to erosion or ulcers. Nishimura et al 44) also reported that HPV infection was frequently observed in the oral mucosa of patients wearing dentures, suggesting that chronic stimulation of the mucosa by the denture and subsequent erosion of the mucosa are the reason for the frequent infection. In the present study, the highest HPV positive rate was observed in the erosive type of OLP.…”

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus in Oral Lichen Planus of Japanese Patients

Kato

Kawai

Isomura

et al. 2015

J. Hard Tissue Biology.

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Oral lichen planus (OLP) can undergo malignant transformation and become squamous cell carcinoma (SCC). Oral infection with human papillomavirus (HPV) is associated with a significant risk of developing oral cancer. Although HPV DNA is detected more often in OLP tissue than in normal oral mucosa, there is as yet no firm evidence that HPV is a causative factor of malignant transformation in OLP. The objective of the present investigation was to assess HPV-genotype distribution in OLPs of Japanese patients and additionally to clarify the relationship between malignant transformation in OLP and HPV infection using PCR, in situ hybridization, and immunohistochemistry. DNA of 200 formalin-fixed, paraffin-embedded biopsy and surgical specimens of OLP was extracted. HPV infection was first detected by PCR using consensus HPV primers. Positive PCR samples were then further analyzed by PCR using HPV type-specific primers (HPV-6, -11, -16, -18 and -33). Eighty-three samples (41.5 %) out of the total 200 OLP specimens analyzed were HPV positive. In the HPV type-specific PCR assay, the most frequent type of HPV was HPV-16 (25.5 %), which is a high-risk type of HPV that is associated with malignant disorders and is often detected in SCC. The highest HPV-16 positive rate was obtained for the erosive type of OLP (28.3 %). Positive staining for HPV DNA by in situ hybridization was observed in the nuclei of cells in all layers of the epithelium in all HPV PCR positive samples. Immunohistochemically, nuclei of cells in the upper layer of the epithelium in all HPV PCR positive samples stained positive for the anti-HPV antibody. These results indicated that HPV-16 was often present in OLP of Japanese patients, especially in the erosive type of OLP, and suggested that HPV infection is a risk factor for malignant transformation in OLP lesions.

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Section: Discussionmentioning

confidence: 99%

Human Papillomavirus in Oral Lichen Planus of Japanese Patients

Kato

Kawai

Isomura

et al. 2015

J. Hard Tissue Biology.

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“…In the first step of the PCR assay, we used the degenerate L1 consensus primers GP5/6 18) and GP5+/6+ 19) , which can detect the presence of DNA of 9 HR-HPV types (16,18,31,33,35,45, 51, 56 and 58) and seven LR-HPV types (1,6,8,11,13,30,32 and 58) ( Table 1). Secondly, the positive PCR samples in the first step of the assay were analyzed by type-specific PCR with primers designed for the detection of HPV types 6, 11, 16, 18 and 33 13) ( Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…Secondly, the positive PCR samples in the first step of the assay were analyzed by type-specific PCR with primers designed for the detection of HPV types 6, 11, 16, 18 and 33 13) ( Table 1). The HPV genotyping procedure employed in this study was confirmed by direct DNA sequence HPV.…”

Section: Methodsmentioning

confidence: 99%

“…Positive specimens were analyzed by type-specific-PCR for HPV types 6, 11, 16, 18 and 33 infection 13) , as previously described (Table 1). These oligonucleotide primers were synthesized by SigmaAldrich Japan (Tokyo, Japan).…”

Section: Pcr Analysismentioning

confidence: 99%

“…These lesions may be due to acute or chronic reactions to microbial denture plaque, the denture base materials, or mechanical denture injury 7) . Some investigators have reported that HPV infection is related to certain factors, including the sexual act, gender, age, smoking habit, alcohol consumption and denture wearing [9][10][11][12][13] . Denture epulis has been described as denture fibroma, epulis fissuratum, denture fibrosis, denture injury tumor, or hyperplasia from denture irritation 7,[14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

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Hyperplastic Epithelium of Oral Mucosal Lesions Associated with Denture Wearing as a Potential Reservoir of Human Papillomavirus

Erkhembaatar

Kawai

Kato

et al. 2014

J. Hard Tissue Biology.

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To date, more than 120 total types of HPV have been identified, and in a recent meta-analysis, HPV was confirmed as an independent risk factor for oral carcinoma. Some investigators have reported that HPV infection is related to certain factors, including the gender, age, alcohol consumption, smoking habit, sexual behavior and denture wearing. Denture wearing can lead to denture epulis, which is a hyperplasia of fibrous connective tissue caused by denture irritation. Recently, HPV infection was detected in the hyperplastic epithelium of denture fibroma, but it still has received little study. The objective of the present investigation, therefore, was to clarify the relationship between the hyperplastic epithelium of the denture epulis and HPV infection. DNA of 118 formalin-fixed, paraffin-embedded hyperplastic epithelium biopsies of epulis specimens was extracted. Firstly, HPV infection was detected by PCR using consensus primers. Secondary, PCR using HPV type-specific primers (low risk types 6 and 11; high risk types 16, 18 and 33) was done in positive PCR samples. HPV infection was also detected by in situ hybridization and immunohistochemical techniques. Eighteen (23.1 %) of the 78 cases of denture epulis were positive, and 2 (5.0 %) of the 40 cases of non-denture epulis were positive. The difference was statistically significant (p<0.05, using Fisher's exact test). The most frequent type of HPV found in the 14 positive samples was HPV 16. It seems that the hyperplastic epithelium of denture epulis is easily infected with viruses because the epithelium is exposed daily to traumatic irritation from dentures. These results suggest that the hyperplastic epithelium of denture epulis might be an important reservoir for HPV infection of the oral region where later HPV-associated diseases, such as oral cancer and other oral lesions, may develop.

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Prevalence and risk factors of oral HPV infection among a healthy Iranian population; Kurdistan, West of Iran

Zargaran

Jalilian

2020

Journal of Medical Virology

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Human papillomavirus (HPV) infection has implicated in the development of some of the oral/oropharyngeal cancers. However, controversy still exists regarding the prevalence of oral HPV (OHPV) and its risk factors. This study aimed to determine the prevalence and variables of OHPV infection in a healthy Iranian population. This study evaluated 300 oral rinse samples. Following the oral and dental examination of participants and filling out a self‐administered questionnaire; samples collected by swishing and gargling 0.09% saline. The viral DNA extraction, polymerase chain reaction and HPV genotyping then performed. Prevalence of OHPV DNA/OHPV+ infection and OHPV genotypes was 12% and 1% (two cases of HPV6 and one case of HPV53), respectively. Comparison of variables between OHPV+ and OHPV− groups revealed that only income (P = .045), number of cigarettes smoked per day (P = .002), and number of teeth in the mouth (P = .005) were significantly different between the two groups. In conclusion, prevalence of OHPV+ infection and its genotypes were very low in our healthy Iranian population, and its association was not significant with the majority of suggested risk factors. Further studies with a larger sample size are recommended to determine OHPV infection risk factors.

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Human Papillomavirus Infection in the Oral Cavity of Denture Wearers

Cited by 13 publications

References 0 publications

Human Papillomavirus in Oral Lichen Planus of Japanese Patients

Human Papillomavirus in Oral Lichen Planus of Japanese Patients

Hyperplastic Epithelium of Oral Mucosal Lesions Associated with Denture Wearing as a Potential Reservoir of Human Papillomavirus

Prevalence and risk factors of oral HPV infection among a healthy Iranian population; Kurdistan, West of Iran

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