Human papillomavirus infection and cervical cancer precursor lesions in women living by Amazon rivers: investigation of relations with markers of oxidative stress

Abstract

Objective To investigate the relation between oxidative stress markers, human papillomavirus infection and cervical cancer precursor lesions.Methods The study comprised women aged 14 to 60 years living in communities located by Amazon rivers in the state of Pará (Itaituba, Limoeiro do Ajuru and Bragança, 126, 68 and 43 women respectively). Papanicolau smears and polymerase chain reaction tests for human papillomavirus DNA detection were performed. Blood samples were collected to test malondialdehyde, total and oxidized glutathione levels.Results Malondialdehyde, total and oxidized glutathione concentrations did not differ significantly (p>0.05) between women with and without low-grade squamous intraepithelial lesions across communities. Malondialdehyde levels (8.02nmols/mL) were almost five times higher in human papillomavirus-positive compared to human papillomavirus-negative women (1.70nmols/mL) living in Itaituba (statistically significant difference; p<0.05). Malondialdehyde levels did not differ significantly (p>0.05) between human papillomavirus-positive and human papillomavirus-negative women living in remaining communities. Significant (p<0.05) differences in total glutathione levels between human papillomavirus-positive and human papillomavirus-negative women (8.20μg/mL and 1.47μg/mL, respectively) were limited to those living in Bragança.Conclusion Malondialdehyde and total glutathione levels were significantly associated with human papillomavirus infection. However, lack of similar associations with squamous lesions suggest oxidative stress alone does not explain correlations with cervical carcinogenesis. Other factors may therefore be involved.

The vaginal metabolome and microbiota of cervical HPV‐positive and HPV‐negative women: a cross‐sectional analysis

“…These metabolites may represent oxidative stress and total glutathione depletion. By‐products of oxidative stress may cause irreversible damage to membrane lipids, proteins, and DNA, and have been implicated as potential co‐factors in HPV carcinogenesis and persistence . Future longitudinal studies designed to establish the association of these compounds with disease progression, in combination with specific hrHPV strains, are merited.…”

Knockdown of BRCC3 exerts an anti‑tumor effect on cervical cancer in�vitro

“…Human papillomavirus (HPV) infection is one of the leading causes of cervical cancer and is associated with a majority of cervical cancer cases ( 8 , 9 ). The primary causes of mortality associated with cervical cancer are unsuccessful surgical treatment, tumor recurrence and metastasis ( 10 ).…”

Evaluation of Dynamic Thiol-Disulfide Balance in Preinvasive Lesions of the Cervix

“…In these studies, plasma total glutathion levels were reported as low and oxidized glutathione levels were reported as high in patients with preinvasive lesions compared to patients without preinvasive lesions [17,18]. They added that this relationship may be related to HPV positivity.…”

“…Also, HPV increases the ability of infected cells to live in an oxidative environment [23]. In some of the studies conducted on cervical preinvasive lesions in the literature, oxidative stress indicators were associated with HPV, while the relationship between preinvasive lesions were not determined [18]. In addition, there are investigations showing that oxidative stress is associated with both HPV and dysplasia grade [24].…”

“…We used a commercial kit; digene HC2 HPV DNA Test (Qiagen Germantown, Inc., MD, USA) for HPV typing. This kit can detect 13 types of high-risk HPVs (16,18,31,33,35,39,45,51,52,56,58,59,68) and 5 types of low-risk HPVs (6,11,42,43,44).…”