Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Coser, Janaina; Boeira, Thaís da Rocha; Fonseca, André Salvador Kazantzi; Ikuta, Nilo; Lunge, Vagner Ricardo

doi:10.1590/s1413-86702011000500009

Cited by 3 publications

(3 citation statements)

References 28 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results of the present study identified a HPV infection prevalence of 34% in women attending a public health service in Cruz Alta. This result is, therefore, amongst the highest HPV prevalence values observed in Brazil to date and likely reflects the use of nested-PCR assays for data gathering, a method that has a higher analytical sensitivity compared with other HPV DNA detection strategies (Lipmann et al, 2010;Coser et al, 2011). Our HPV prevalence finding was also slightly higher than those previously found in populations within in the State of Rio Grande do Sul by PCR and hybrid capture analysis (Nonnenmacher et al, 2002;Rosa et al, 2008).…”

Section: Discussionsupporting

confidence: 49%

See 1 more Smart Citation

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

Coser¹,

T²,

Simon³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Human papillomavirus (HPV) infection is a common viral sexually transmitted infection and the main cause of cervical cancer in women worldwide. Epidemiological data on the prevalence of HPV in a given population is essential for the establishment of effective prevention strategies. The aim of this study was to determine HPV prevalence in women who attended a public health service within an urban center in Brazil. Cervical samples were collected from 337 women recruited from a primary public health care clinic in the city of Cruz Alta located in Rio Grande do Sul, the were detected in 83 (72.8%) and 48 (42.1%) samples, respectively. Furthermore, a great diversity of HPV genotypes was observed (18 high-risk, 12 low-risk, and 1 indeterminate). The most commonly identified low-risk types were candHPV62 (7.9%) and 61 (5.3%), while the most common high-risk types were 16 and 33 (8.8% each). Abnormal cytology was observed in 10 (3.0%) women, 9 of which were infected with HPV. Of the remaining 327 women with normal cytology results, 107 (32.7%) were positive for HPV DNA. HPV infection was correlated with younger age (less than 40 years), a first Pap smear, and other vaginal infections.

show abstract

Section: Discussionsupporting

confidence: 49%

“…For HPV DNA detection, genomic DNA was first extracted from samples using the silica method (Boom et al, 1990) and then amplified by nested polymerase chain reaction (nested-PCR) as previously described (Coser et al, 2011). Briefly, nested-PCR was performed with two DNA amplification rounds.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

Coser¹,

T²,

Simon³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…4,5 A infecção persistente por HR-HPV constitui o principal fator de risco para o desenvolvimento de neoplasia cervical 6 , onde cerca de 20-50% das mulheres infectadas são portadoras de múltiplos tipos de HR-HPV. 7 Considerando o importante papel da infecção pelo HPV no desenvolvimento do câncer do colo uterino, este estudo objetivou estimar a frequência da infecção por HPV e identificar os tipos virais mais prevalentes em amostras autocoletadas de mulheres jovens sexualmente ativas.…”

unclassified

Infection with multiple types of human papillomavirus in sexually active young women

Almeida¹,

Machado²,

Almeida³

et al. 2015

Medicina (Ribeirão Preto)

View full text Add to dashboard Cite

Study design: cross. Study objective: estimate the frequency and distribution types of human papillomavirus (HPV) in sexually active young women. Methods: samples of vaginal and endocervical canal of 158 women, aged between 18 and 35 years were collected through self-collection technique. The HPV DNA was extracted and amplified by PCR end point using the consensus primers PGMY09/11 and genotyping was performed by PCR specific type (TS-PCR) and by analysis of the fragments obtained from the use of restriction enzymes (RFLP). Results: HPV DNA was detected in 23% of participants, 92% were high-risk HPV genotypes (HR), being the prevalent HPV45, followed by HPV16 and 31 infections with multiple HPV types were detected in 35% of samples, indicating infection by more than two types in 22.2% of the genotyped. Higher frequency of HPV DNA was detected in women ≤ 25 old. Conclusion: this demonstrated a high frequency of HPV infection in sexually active young women, as well as a high prevalence of infection with multiple types of high oncogenic risk.

show abstract

Molecular approaches for HPV genotyping and HPV-DNA physical status

Tsakogiannis

Gartzonika

Levidiotou-Stefanou

et al. 2017

Expert Rev. Mol. Med.

View full text Add to dashboard Cite

Persistent infection with high-risk human papillomavirus (HPV) genotypes is the leading cause of cervical cancer development. To this end several studies have focused on designing molecular assays for HPV genotyping, which are considered as the gold standard for the early diagnosis of HPV infection. Moreover, the tendency of HPV DNA to be integrated into the host chromosome is a determining event for cervical oncogenesis. Thus, the establishment of molecular techniques was promoted in order to investigate the physical status of the HPV DNA and the locus of viral insertion into the host chromosome. The molecular approaches that have been developed recently facilitate the collection of a wide spectrum of valuable information specific to each individual patient and therefore can significantly contribute to the establishment of a personalised prognosis, diagnosis and treatment of HPV-positive patients. The present review focuses on state of the art molecular assays for HPV detection and genotyping for intra-lesion analyses, it examines molecular approaches for the determination of HPV-DNA physical status and it discusses the criteria for selecting the most appropriate regions of viral DNA to be incorporated in HPV genotyping and in the determination of HPV-DNA physical status.

show abstract

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Cited by 3 publications

References 28 publications

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

Infection with multiple types of human papillomavirus in sexually active young women

Molecular approaches for HPV genotyping and HPV-DNA physical status

Contact Info

Product

Resources

About