Human Papilloma Virus 18 Detection in Oral Squamous Cell Carcinoma and Potentially Malignant Lesions Using Saliva Samples

Abstract

Background: Oral cancer has become one of the most prevalent cancers worldwide and human Papillomavirus is one of the risk factors for developing oral cancer. For this study HPV18 was chosen as it is one of the high risk HPV types and may lead to carcinogenesis. However, prevalence of HPV18 infection in Oral Squamous Cell Carcinoma in Malaysia remains unclear. Objective: This study aimed to investigate the viral load of HPV18 DNA in OSCC and potentially malignant lesions using saliva samples. Materials and Methods: Genomic DNAs of thirty saliva samples of normal subjects and thirty saliva samples compromised of 16 samples from potentially malignant lesions and 14 of OSCC patients were amplified for HPV18 DNA using a nested polymerase chain reaction analysis. All PCR products were then analyzed using the Bioanalyzer to confirm presence of HPV18 DNA. Result: From thirty patients examined, only one of 30 (3.3%) cases was found to be positive for HPV18 in this study. Conclusion: The finding of this study revealed that there is a low viral detection of HPV18 in Malaysian OSCC by using saliva samples, suggesting that prevalence of HPV18 may not be important in this group of Malaysian OSCC.

Prevalence of Human Papillomavirus Infection in Oral Squamous Cell Carcinoma: a Case-control Study in Wuhan, China

“…In the control group, both two HPV positive samples were caused by HPV18. A Malaysia research suggested HPV18 may be not a crucial factor in Malaysian OSCC according to the result of low percentage (1/30) of HPV18 (Goot-Heah et al, 2012). The similar result obtained in the control group in our study indicated other HPV types should be paid more attention.…”

Lack of Association between High-risk Human Papillomaviruses and Oral Squamous Cell Carcinoma in Young Japanese Patients

“…For example, HPV is detected in cervical and oropharyngeal SCC at frequencies of 90% (Harald, 2002;Clifford, 2003;de Sanjose, 2010) and 47.7% (Mehanna, 2013), respectively, with evidence of functional E6 and E7 oncoproteins. The study in India demonstrated a positive correlation of HPV infection with oral tongue cancer (Elango et al, 2011), while HPV may not play an important role in Thai and Malaysian OSCC (Khovidhunkit et al, 2008;Goot-Heah et al, 2012). Therefore, the present study aimed to investigate whether the presence of HPV correlates with OSCC in Japanese patients aged <40 years.…”

Association between the TP53 codon 72 polymorphism and risk of oral squamous cell carcinoma in Asians: a meta-analysis

“…Oral squamous cell carcinoma (OSCC) originates from the squamous cells that cover the surface of the mouth and is a major type of oral cancer, accounting for more than 90% of cases [3]. Tobacco use (chewing with or without smoking), alcohol consumption, and human papillomavirus (HPV) infection are important risk factors for development of OSCC [4,5]; however, molecular mechanisms relating to OSCC are still being investigated, while genetic predisposition is gaining increasing attention [6-8]. …”