Human olfactory stem cells for injured facial nerve reconstruction in a rat model

Batıoğlu-Karaaltın, Ayşegül; Karaaltın, Mehmet Veli; Oztel, Olga Nehir; Ovalı, Ercüment; Sener, Belit Merve; Adatepe, Turgut; Yiğit, Özgür; Bozkurt, Erol Rüştü; Baydar, Serap Yeşilkır; Bağırova, Melahat; Uzun, Nurten; Allahverdiyev, Adil M.

doi:10.1002/hed.24371

Cited by 18 publications

(14 citation statements)

References 64 publications

(156 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Total nerve laceration usually occurs as a result of trauma, tumour excision, or iatrogenic etiologies. Although peripheral motor nerves have regeneration capacity, full healing is not achieved if total nerve laceration is present [Batioglu-Karaaltin et al, 2016]. The outcomes of end-toend anastomosis are not always satisfactory because of poor axonal regeneration and synkinesis [Chu et al, 2018;Edizer et al, 2019].…”

Section: Discussionmentioning

confidence: 99%

Is Decorin a Promising New Agent for Facial Nerve Regeneration? An Experimental Study

Çınar¹,

Emre²,

Gül

et al. 2021

Audiol Neurotol

View full text Add to dashboard Cite

Objective: The aim of this study was to investigate the effects of systemic administration of decorin (DC) on facial nerve (FN) regeneration. Methods: A total of 32 female albino Wistar rats were divided into 4 groups: control (C) group: no bilateral FN neurorrhaphy (B-FNN), no DC application, sham-operated group: B-FNN without DC application, DC group: DC application without B-FNN, and B-FNN + DC group: B-FNN and DC application. Nerve conduction studies were performed before and after skin incisions at 1st, 3rd, 5th, and 7th weeks in all groups. The amplitude and latency of compound muscle action potentials were recorded. FN samples were obtained and were investigated under light microscopy and immunohistochemical staining. The nerve and axon diameter, number of axons, H score, Schwann cell proliferation, and myelin and axonal degeneration were recorded quantitatively. Results: In the sham group, the 3rd and 5th postoperative week, amplitude values were significantly lower than those of the B-FNN + DC group (p < 0.05). Nerve diameters were found to be significantly larger in the sham, DC, and B-FNN + DC groups than in the C group (p < 0.05). The number of axons, the axon diameter, and the H scores were found to be significantly higher in the B-FNN + DC group than in the sham group (p < 0.05). The Schwann cell proliferation, myelin degeneration, and axonal degeneration scores were significantly lower in the B-FNN + DC group than in the sham group (p < 0.05). Conclusion: Electrophysiological and histopathological evaluation revealed the potential benefits provided by DC. This agent may increase FN regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Is Decorin a Promising New Agent for Facial Nerve Regeneration? An Experimental Study

Çınar¹,

Emre²,

Gül

et al. 2021

Audiol Neurotol

View full text Add to dashboard Cite

show abstract

“…показали, що застосування нейрогенних стовбурових клітин справляє позитивний ефект не тільки при гострій травмі нерва, а і при хронічній денервації, що підтверджувалося електрофізіологічними та морфологічними методами [9,85]. Застосування їх обмежується тому, що використання нейральних стовбурових клітин при різних видах травми ПН супроводжується високим ступенем утворення нейробластом [86], тому увагу дослідників привернули більш диференційовані клітини, а саме клітини нюхової цибулини [87]. Виявлено, що вони здатні продукувати речовини, які стимулюють регенерацію ПН: FGF, NGF, BDNF, GDNF, білки екстра целюлярного матриксу [88,89].…”

Section: джерела і види стовбурових клітин для відновлення периферичнunclassified

Stem Cell Technology in Peripheral Nerve Restoration

Petriv

Tsymbalyuk

Potapov

et al. 2020

EUMJ

View full text Add to dashboard Cite

Peripheral nerve injuries are a significant problem in the medical and socio-economic plan, as they are accompanied by a high incidence of disability by people of working age. In recent decades, significant progress has been made in the restorative surgery of the peripheral nervous system, in particular through the introduction into clinical practice of microsurgical techniques. However, the problem of restoring the peripheral nerve after its traumatic injury has not been resolved yet. A review article addresses the current state of developing stem cell technologies for peripheral nerve repair. Basic concepts of peripheral nerve regeneration after traumatic injury, methods of their restoration in experimental and clinic conditions are considered. The prospect of using stem cells of different origins is shown in the experiment by many authors, and the positive effect of stem cells on peripheral nerve regeneration is explained by their ability to secrete many trophic factors and differentiation to a neural phenotype. An essential issue in the tissue engineering approach is the choice of the optimal material to be used as a scaffold for large size peripheral nerve defects grafting. The article focuses on the main types of stem cells, as well as their combinations with biopolymers, which have shown efficiency in the experiment. Despite the advances in the use of the latest technologies, the search for the necessary components is underway to provide the most favorable conditions for peripheral nerve regeneration in the clinic.

show abstract

“…There are studies using adult, embryo and fetal stem cells for the regeneration of nerve damage. Many subtypes of these stem cells, such as bone marrow, mesenchymal and neural stem cells have been used for regeneration in peripheral nerve damage [2,9,13,14].In previous years, there are animal studies carried out using many kind of stem cells in the FP that reported good results [15][16][17][18][19][20][21][22]. There is no research in the literature about the effects of fetal brain tissue-oriented mesenchymal stem cells (FBTMSC) on facial nerve damage.…”

Section: Introductionmentioning

confidence: 99%

IMPACT OF FETAL BRAIN TISSUE DERIVED MESENCHYMAL STEM CELL and FIBRIN GLUE ON FACIAL NERVE CRASH INJURY

et al. 2021

View full text Add to dashboard Cite

Impact of fetal brain tissue derived mesenchymal stem cell and fibrin glue on facial nerve crash injury Background/aim: To evaluate the clinical and histopathological effects of fetal brain tissue derived mesenchymal stem cells(FBTMSC) and fibrin glue(FG) on the facial nerve(FN) regeneration in rats with traumatic FN injury. Materials and methods: 28 Sprague Dawley rats were included in the study and divided into 4 groups. Traumatic FN injury(FP) was created by a surgical clamp compression to the main trunk of left FN in all groups. In the control group(Group 1) no treatment was applied, in Group 2(FBTMSC group)2x10 6 FBTMSC was injected, in Group 3(FG group) only FG was applied, in group 4(FBTMSC & FG group) both FBTMSC and FG was applied to the injured section of the nerve. The FN functions were evaluated clinically, immediately after the procedure and at 3 rd-5 th-8 th weeks postoperatively. The FNs of all subjects were excised after the 8 th week; then the rats were sacrificed. The presence of stem cells in the injured zone was assessed by using BrdU(Bromo-deoxyuridine), and apoptosis was determined by TUNEL method. Results: After the damage, total FP was observed in all subjects. Statistically significant functional improvement was observed in Group 4 compared to all other groups(p<0.005). TUNEL positive cell count was statistically significantly higher in the control group than the other groups(p<0.001). TUNEL positive cell count was statistically significantly lower in Group 4 than the other groups. The proportion of BrdU-stained cells in Group 4(5%) was higher than Group 2(2%). 2 Conclusion: Clinically and histopathologically FBTMSC applied with FG may play a promising role as a regenerative treatment in posttraumatic FP.

show abstract

Human olfactory stem cells for injured facial nerve reconstruction in a rat model

Abstract: This study suggests that olfactory stem cells may be used as a potent cellular therapy for accelerating the regeneration of peripheral nerve injuries. © 2016 Wiley Periodicals, Inc. Head Neck 38: E2011-E2020, 2016.

Cited by 18 publications

References 64 publications

Is Decorin a Promising New Agent for Facial Nerve Regeneration? An Experimental Study

Is Decorin a Promising New Agent for Facial Nerve Regeneration? An Experimental Study

Stem Cell Technology in Peripheral Nerve Restoration

IMPACT OF FETAL BRAIN TISSUE DERIVED MESENCHYMAL STEM CELL and FIBRIN GLUE ON FACIAL NERVE CRASH INJURY

Contact Info

Product

Resources

About