Human mitochondrial DNA and nuclear DNA isolation from food bite marks

Zapico, Sara C.; Menéndez, Sofía T.

doi:10.1016/j.archoralbio.2016.06.004

Cited by 6 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additionally, the formula for their extraction buffer is provided, making it easier to assess and apply the appropriate DNA extraction method, avoiding any interferences or inhibitions with the kit’s reagents. The DNEasy Blood & Tissue kit was chosen because of its simple and easy-to-use protocol, and previous publications from our lab demonstrated its successful performance with tough samples like teeth, food bite marks, and contact lenses, only requiring a modification of the protocol depending on the substrate [ 21 , 22 , 23 ].…”

Section: Expected Resultsmentioning

confidence: 99%

Making the Most of Lateral Flow Immunochromatographic Tests: An Efficient Protocol to Recover DNA

Zapico,

Roca

2024

MPs

View full text Add to dashboard Cite

Lateral flow immunochromatographic (LFI) tests are widely used in both biomedical and forensic sciences for different applications. In forensic sciences, their main use is to detect body fluids at crime scenes. However, there are situations in which the amount of potential biological evidence is so low that DNA extraction is favored with respect to the identification of body fluids. Here, an efficient and quick protocol is presented to integrate the detection of body fluids through LFI with DNA extraction from a sample swab and buffer, providing a complete characterization of the biological evidence. This protocol is a modification of a general DNA extraction silica-based kit, whose main application is for blood and tissues. Thus, it could be carried out in different settings (forensic labs, hospitals, other testing labs) without the necessity of buying a specific kit for swabs. The validation of this protocol is supported by the results presented here and previous publications from our group, obtaining DNA in good quantity and with good quality. This proves the potential application of the protocol in both forensic scenarios, to fully characterize biological evidence, and biomedical settings, to molecularly confirm the results of LFI tests.

show abstract

Section: Expected Resultsmentioning

confidence: 99%

Making the Most of Lateral Flow Immunochromatographic Tests: An Efficient Protocol to Recover DNA

Zapico,

Roca

2024

MPs

View full text Add to dashboard Cite

show abstract

“…Mold reduced the amount of amplifiable DNA but did not rule out a successful STR analysis [ 131 ]. Earlier studies indicated that saliva DNA could be preserved on bite marks for 16 [ 132 ] and 24 h [ 133 ].…”

Section: Degradation Factorsmentioning

confidence: 99%

Nucleic Acids Persistence—Benefits and Limitations in Forensic Genetics

Żarczyńska,

Żarczyński,

Tomsia

2023

Genes

View full text Add to dashboard Cite

The analysis of genetic material may be the only way to identify an unknown person or solve a criminal case. Often, the conditions in which the genetic material was found determine the choice of the analytical method. Hence, it is extremely important to understand the influence of various factors, both external and internal, on genetic material. The review presents information on DNA and RNA persistence, depending on the chemical and physical factors affecting the genetic material integrity. One of the factors taken into account is the time elapsing to genetic material recovery. Temperature can both preserve the genetic material or lead to its rapid degradation. Radiation, aquatic environments, and various types of chemical and physical factors also affect the genetic material quality. The substances used during the forensic process, i.e., for biological trace visualization or maceration, are also discussed. Proper analysis of genetic material degradation can help determine the post-mortem interval (PMI) or time since deposition (TsD), which may play a key role in criminal cases.

show abstract

“…Já a queiloscopia, envolve o estudo das formas, depressões e elevações das impressões labiais (Chugh & Narwal, 2017). O DNA também pode ser utilizado em alguns casos, afinal ele está presente no sangue e também na saliva, sendo assim, durante o processo de mordida a saliva pode ser depositada sobre a pele ou objeto, e quando presente em quantidades suficientes, pode revelar a identidade genética do suspeito (Zapico & Menéndez, 2016).…”

Section: Odontologia Forenseunclassified

Identificação da análise de marcas de mordida em odontologia forense: uma revisão da literatura

Melo

Costa

Pereira

et al. 2021

RSD

View full text Add to dashboard Cite

A Odontologia Forense é uma área das ciências forenses responsável por ajudar a justiça principalmente nos casos de identificação humana. As técnicas de identificação remontam desde a época de 49 DC, e seu uso vem sendo relatado ao longo da história. A análise através das marcas de mordida é uma técnica complexa e que traz muitas subjetividades, dividindo opiniões e gerando muitas controvérsias. O presente trabalho consiste de uma revisão de caráter narrativo e discute como é feita a identificação através das marcas de mordida, destacando sua importância e suas limitações, apontando também o papel do cirurgião-dentista. Foi realizada uma busca nas bases de dados “PubMed”, “Scielo”, e “Lilacs” dos últimos 20 anos utilizando os descritores em português: “Mordida Humana”, “Odontologia Forense”, “Mordida" e em inglês “Bite Human”, “Forensic dentistry” e “Bite”. Essas marcas são encontradas principalmente em crimes de abuso sexual, homicídio e negligencia infantil, onde o criminoso utiliza os dentes como arma, deixando uma marca sobre a pele da vítima ou em objetos deixados na cena do crime. Partindo do princípio de que a arcada dentaria é única para cada ser humano, é feita uma comparação entre a marca deixada no local com a dentição do suspeito, podendo incriminar o indivíduo. Diante disso, conclui-se que as marcas de mordida podem ser uma importante ferramenta na resolução de crimes, desde que realizada de maneira criteriosa, por profissionais qualificados e respeitando suas limitações.

show abstract

Human mitochondrial DNA and nuclear DNA isolation from food bite marks

Cited by 6 publications

References 21 publications

Making the Most of Lateral Flow Immunochromatographic Tests: An Efficient Protocol to Recover DNA

Making the Most of Lateral Flow Immunochromatographic Tests: An Efficient Protocol to Recover DNA

Nucleic Acids Persistence—Benefits and Limitations in Forensic Genetics

Identificação da análise de marcas de mordida em odontologia forense: uma revisão da literatura

Contact Info

Product

Resources

About