Human intestinal diamine oxidase: Substrate specificity and comparative inhibitor study

Biegański, Tadeusz; Kusche, Jürgen; Feussner, Klaus D.; Hesterberg, R.; Richter, Hans G.; Lorenz, W.

doi:10.1007/bf02024190

Cited by 24 publications

(17 citation statements)

References 18 publications

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“…Human intestinal DAO closely resembles other mammalian diamino oxidases in phys ical and chemical properties, such as specific ity for substrates and inhibitors [31], and our data confirm these biological analogies with regard to liver uptake as well. Our results, in conclusion, suggest that the liver plays an important role in uptake and, probably, in metabolism of plasma DAO also in man.…”

Section: Discussionsupporting

confidence: 80%

Metabolic Fate of Plasma Diamine Oxidase: Evidence of Isolated and Perfused Rat Liver Uptake

D’Agostino¹,

Ciacci²,

Capuano³

et al. 1986

Digestion

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After injection of an intravenous bolus of heparin (15,000 IU) in two groups of subjects, 10 normal volunteers and 6 subjects with external biliary drainage, blood and urine samples were collected; in the latter group bile samples were collected also. All samples were assayed for diamine oxidase (DAO). Persistently high values of this enzyme were found in plasma of both populations after heparin stimulation, while no increase in enzymatic activity was detected in bile and urine. In order to confirm and support the hepatic clearance of DAO, liver uptake of the enzyme derived from porcine kidney, human plasma and human placenta was studied by perfusion of isolated rat liver. Disappearance curves of the enzyme derived from three different sources showed a prompt liver uptake: activity decreased by about 50% in 10min (endocytic uptake) and a slower but constant reduction during the remaining 110 min of perfusion was observed. These data suggest the hypothesis of liver metabolism of plasma DAO.

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Section: Discussionsupporting

confidence: 80%

Metabolic Fate of Plasma Diamine Oxidase: Evidence of Isolated and Perfused Rat Liver Uptake

D’Agostino¹,

Ciacci²,

Capuano³

et al. 1986

Digestion

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“…In this connection it was especially intriguing that human intestinal DAO not only deaminated histamine but even with a higher reaction velocity N~-methylhistamine, the strong inhibitory product of the other histamineinactivating enzyme HMT [32]. Thus our in vitro results support the hypothesis that DAO has a histamine-detoxicating function in the intestinal tract.…”

Section: The Role Of Mao In Histamine Catabolismsupporting

confidence: 76%

Intestinal monoamine oxidase: Does it have a role in histamine catabolism?

1982

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The importance of intestinal diamine oxidase in histamine catabolism was proved in several series of experiments. However, intestinal monoamine oxidase might also be involved in histamine degradation either by direct deamination or by the deamination of methylated products. The soluble fraction of intestinal monoamine oxidase was purified and tested for the properties and substrate specificity by three different methods which are described in detail. Using 0.15 M phosphate buffer the optimum pH was 7.4--7.6. The Km values for serotonin and tyramine were 0.2 and 0.3 X 10(-3) M. The most favoured substrates of the enzyme were tyramine, tryptamine and serotonin, but it was not possible to classify the enzyme as a type A or B monoamine oxidase only by its substrate specificity. Histamine and ring methylated derivatives were not attacked by intestinal monoamine oxidase. This means that in the intestinal mucosa by the oxidative pathway of histamine is completely catalysed by diamine oxidase.

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“…Es wur den schon vie le Stu dien zum Thema His ta mi nin to le ranz und Kor re la ti on zu einer re du zier ten DAO-Ak ti vi tät ver öf fent licht [15,18,20,21]. Die Di ag no se HIT wird norma ler wei se ge stellt, wenn ty pi sche HITSymptome auf tre ten, die sich nach ei ner hista min frei en Diät bes sern [22].…”

Section: Diskussionunclassified

“…Beim Ge sun den findet die oxi da ti ve De sa mi nie rung durch das En zym Di amin oxi da se schnell statt, so dass nor ma ler wei se kei ne Symp to me auf tre ten [2]. Die DAO, das En zym das haupt säch lich für den Ab bau von His ta min und an de ren bioge nen Ami nen ver ant wort lich ist [2,14,15], wird in dif fer en zier ten Epit hel zel len ex primiert. Die höch sten Ak ti vi tä ten lie gen da bei in der Dünn darm schleim haut und im Co lon as cen dens [2,16,17,18].…”

Section: Introductionunclassified

Histaminintoleranz: Wie sinnvoll ist die Bestimmung der Diaminoxidase-Aktivität im Serum in der alltäglichen klinischen Praxis?

Töndury¹,

Wüthrich²,

Schmid‐Grendelmeier³

et al. 2008

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(2008)His ta mi nin to le ranz: Wie sinn voll ist die Be stim mung der Di amin oxi da se-Ak tivi tät im Se rum in der all tägli chen kli nischen Praxis?Hin ter grund: Eine Nah rungs mit tel un verträg lich keit kann durch ei nen ver min der ten en zy ma ti schen His ta min ab bau auf grund einer ver rin ger ten Ak ti vi tät der Di amin oxi da se (DAO) in du ziert sein (His ta mi nin to le ranz (HIT)). Ziel set zung: Ziel der vor lie gen den Stu die war es he raus zu fin den, ob die DAOAktivität im Se rum bei Pa tien ten, de ren Vorge schich te eine HIT ver mu ten lässt, im Vergleich zu Pa tien ten ohne eine Vor ge schich te mit HIT sig ni fi kant ver rin gert ist. Me tho den: Un ter sucht wur den 61 Pa tien ten und 20 Kontrol len. Bei 26 Pa tien ten (schwere HIT) lag eine Vor ge schich te mit min de stens zwei ty pischen HIT-Symp to men in Zu sam men hang mit der Auf nah me von min de stens zwei his tamin rei chen Nah rungs mit teln vor. Bei 35 Patien ten (mä ßi ge HIT) war nach der Auf nah me von min de stens ei nem his ta min rei chen Nahrungs mit tel min de stens ein HIT-Symp tom auf ge tre ten. Die 20 ge sun den Kon trol len zeig ten kei ner lei HIT-Symp to me. Die Pa tienten wur den zur Art der kli ni schen Symp to me und zum Zu sam men hang der Symp to me mit der Auf nah me der Nah rungs mit tel be fragt. Die DAO-Aktivität im Se rum wur de mit tels ELI SA ana ly siert. Er geb nis se: Es konn ten kei ne Un ter schie de be züg lich der DAO-Konzen tra ti on im Se rum zwi schen den Grup pen fest ge stellt wer den. Schluss fol ge rung: Ba sierend auf der Ana mne se mit al ler gie ähn li chen Symp to men nach Auf nah me von histaminreichen Nah rungs mit teln bie tet die Bestim mung der DAO-Ak ti vi tät im Se rum in der all täg li chen kli ni schen Pra xis kei ne Hilfe stel lung für die Di ag no se ei ner HIT.His ta mine in tol er ance: Is the de ter mina tion of diamine oxidase ac tiv ity in the serum use ful in rou tine clin i cal prac tice?Back ground: An in tol er ance to food might be in duced by an im paired en zy matic his ta mine deg ra da tion due to a de fi ciency of diamine oxidase (DAO) ac tiv ity (His ta mine in tol er ance [HIT]). Ob jec tive: The aim of the study was to in ves ti gate if pa tients his to ries, that are sug ges tive to HIT have a sig nif i cant re duced se rum DAO ac tiv ity com pared to patients with out his tory of HIT. Meth ods: 61 patients and 20 con trols were stud ied. 26 patients (strong HIT) had a his tory of at least two typ i cal symp toms of HIT with re la tion to the in take of at least two his ta mine rich foods. 35 pa tients (mod er ate HIT) had a his tory of at least one symp tom af ter the in take of at least one his ta mine rich food (but not both). 20 healthy vol un teers (con trol) did not have any symp toms of HIT. Pa tients were in ter viewed on type of clin i cal symp toms and the re la tion of symp toms to food in ges tion. Sera were ana lysed for DAO ac tiv ity by an ELISA test. Re sults: No dif fer ence of se rum DAO lev els could be found be tween groups o...

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Human intestinal diamine oxidase: Substrate specificity and comparative inhibitor study

Cited by 24 publications

References 18 publications

Metabolic Fate of Plasma Diamine Oxidase: Evidence of Isolated and Perfused Rat Liver Uptake

Metabolic Fate of Plasma Diamine Oxidase: Evidence of Isolated and Perfused Rat Liver Uptake

Intestinal monoamine oxidase: Does it have a role in histamine catabolism?

Histaminintoleranz: Wie sinnvoll ist die Bestimmung der Diaminoxidase-Aktivität im Serum in der alltäglichen klinischen Praxis?

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