Human herpesvirus 7, Epstein–Barr virus and human cytomegalovirus in periodontal tissues of periodontally diseased and healthy subjects

Rotola, Antonella; Cassai, E.; Farina, Roberto; Caselli, Elisabetta; Gentili, Valentina; Lazzarotto, Tiziana; Trombelli, Leonardo

doi:10.1111/j.1600-051x.2008.01301.x

Cited by 53 publications

(83 citation statements)

References 42 publications

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“…7,18,19 Although few studies showed similar detection rates of HCMV as in our study, there has been a wide and varied report with high levels of viruses to none in certain population with healthy periodontium. 7,9,17,18,[20][21][22][23] In CP, there have been varied reports with some studies finding very high levels of EBV and HCMV. 10,18,20,21,23 Our results showed significantly higher levels of both the HCMV and the EBV virus in CP as compared to the healthy periodontium but still was lower than the median range as quoted by Slots 2010.…”

Section: Discussionmentioning

confidence: 99%

“…7,9,17,18,[20][21][22][23] In CP, there have been varied reports with some studies finding very high levels of EBV and HCMV. 10,18,20,21,23 Our results showed significantly higher levels of both the HCMV and the EBV virus in CP as compared to the healthy periodontium but still was lower than the median range as quoted by Slots 2010. 2 In his study, we noted that a co-infection of HCMV and EBV existed in CP group and this relationship was significant.…”

Section: Discussionmentioning

confidence: 99%

“…The findings of the present study were at lower levels as compared to some studies done in other countries. 18,[20][21][22][23] This could also be because the detection levels may depend on the virus being in the active or latent stage. The primary infection of the host with herpesvirus is followed by a state of latency and reactivation.…”

Section: A Study Done By Contreras Et Al In American Patientsmentioning

confidence: 99%

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Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis

Joshi¹,

Bhat²,

Katti³

et al. 2015

The Journal of Contemporary Dental Practice

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Objective: The identification of new uncultured species and viruses supports the possibility of combination of the herpesvirus-bacterial periodontal infection for periodontitis. The paucity of data and studies with larger sample size in Indian subjects provides an unclear picture of the presence of the herpesvirus in this population. Materials and methods:This was a cross-sectional study consisting of 100 each in the healthy group and chronic periodontitis (CP) group. The subgingival plaque was collected and polymerase chain reaction was performed post deoxyribonucleic acid (DNA) extraction by using specific primers for human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). The data were analyzed using Fisher's exact test, Mann-Whitney U test and Spearman's coefficient correlation.Results: Human cytomegalovirus and EBV viruses were significantly higher in the CP group as compare to the healthy group. A higher percentage of those with CMV positive had EBV also positive (28.3%) compared to only 9.1% of CMV negative being EBV positive in the CP group. When both the healthy and CP group in total was compared, there was a significant correlation with all clinical parameters. Conclusion:Both the viruses dominated in disease as compared to health were similar to the earlier findings. The CP group had higher pocket depth and clinical attachment loss in the virus positive subjects. These findings could suggest that 91-9620127630, e-mail: drvinayakjoshi@gmail.com virus serves as a prelude to the disease and the combination of the two viruses could play a role in the pathogenesis. Prevalence of Herpesvirus and Correlation with Clinical

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: A Study Done By Contreras Et Al In American Patientsmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis

Joshi¹,

Bhat²,

Katti³

et al. 2015

The Journal of Contemporary Dental Practice

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“…Studies have shown that the genotype distribution and seroprevalence of EBV and HCMV differ among populations [8,13]. Additionally, molecular studies have directly compared the endodontic microbiota of patients from different geographic locations and found significant differences in the prevalence of some important species [3,14].…”

Section: Research Articlementioning

confidence: 99%

Herpesviral and “Red Complex” Bacterial Analysis of Acute Apical Abscesses

Ozbek¹

2015

J Dent Probl Solut

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Introduction: To investigate the possible association between presence of Epstein-Barr virus, human cytomegalovirus and three endodontic bacterial pathogens (Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia) "red complex" in samples from patients with acute apical abscesses using real-time PCR.Methods: Periapical samples were collected from 18 acute apical abscess specimens and 6 control samples. The apical area of each study tooth was examined in periapical radiographs obtained using a long cone paralleling technique. The study included only single-rooted teeth that had carious lesions, necrotic pulps, and radiographic evidence of periradicular bone loss. No apparent communication from the abscess to the oral cavity or skin surface was observed. DNA extracts from purulent exudate aspirates of acute apical abscess and six control samples were evaluated for the presence of microbial and viral loads using real-time polymerase chain reaction (PCR) following the kit protocols recommended by the manufacturers. The chi squared test was used for statistical analysis.Results: HCMV, EBV, T. denticola, P. gingivalis, and T. forsythia were detected in 16.7 %, 5.6 %, 44.4 %, 27.7 %, and 22.2 % of the samples, respectively. As for the healthy pulps used as noninflamed controls, no control specimens contained bacterial or viral DNA. The pair HCMV/ T. denticola and HCMV/ T. forsythia was detected in one case. The association between HCMV, T denticola and T. forsythia was not statistically significant. Conclusion:Within the limitations of the present study, our PCR based findings revealed that in "red complex" bacteria, T. denticola and T. forsythia tended to occur in co-infection with HCMV.

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“…5 The prevalence of herpes simplex virus type I (HSV-1) and Epstein-Barr virus (EBV) is reported to be associated with periodontitis and PI. 6,7 However, the pathogenetical mechanisms are still not clearly understood. 8 The prevalence of herpes viruses is extremely high and…”

Section: Introductionmentioning

confidence: 99%

Herpes Simplex 1 and Periopathogen Role in Peri-implantitis

Parthiban

Ahmed

Ramakrishnan

et al. 2017

The Journal of Contemporary Dental Practice

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Introduction:The objectives of this study were to compare the qualitative and quantitative profiles of herpes simplex virus type I (HSV-1) in implant surfaces between participants with periimplantitis (PI) and Healthy peri-implant tissues and to quantitatively assess the relation between HSV-1 and periopathogens inside the microbiological profile associated with PI. Materials and methods:A total of 40 patients with PI and 40 with healthy peri-implant tissues (HI) were recruited. Plaque samples from peri-implant sulcus and internal implant connections were analyzed using quantitative real-time polymerase chain reaction to detect and quantify HSV-1 and periodontopathogens. Frequencies of detection and levels of microorganisms were compared between PI and HI; the frequencies and levels of periodontopathogens were compared between HSV-1+ and HSV-1− PI to assess qualitative relations between HSV-1 and bacteria. Correlation between HSV-1 and periodontopathogens levels was assessed in PI and HI.Results: A total of 77 dental implants affected by PI, and 113 HIs were included. The HSV-1 prevalence was slightly higher in PI compared with controls (33.3 vs 23.8%; p > 0.05); HSV-1 was detected in external samples more frequently compared with internal samples. The HSV-1-positive patients revealed higher median loads of Prevotella intermedia (Pi) and Campylobacter rectus (Cr) compared with HSV-1-negative patients. In the PI group, a significant positive correlation was evidenced between HSV-1 and Tannerella forsythia, Parvimonas micra (Pm), Fusobacterium nucleatum, and Cr levels, while in the HI, positive correlation between HSV-1 and Aggregatibacter actinomycetemcomitans, Pi, and Pm was established. Conclusion:The HSV-1 prevalence cannot be used to identify PI. The HSV-1 was found in similar levels of PI and HI patients after an average of 6 years of loaded implants. The HSV-1 prevalence cannot be used to identify implants with or without the presence of PI. HerpesClinical significance: Although HSV-1 is detected in PI site, HSV-1 may represent an unspecific indicator for the host response to the bacterial challenge observed in PI.

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Human herpesvirus 7, Epstein–Barr virus and human cytomegalovirus in periodontal tissues of periodontally diseased and healthy subjects

Abstract: The results indicate that (i) gingival tissues can be considered a potential reservoir for HHV-7; (ii) when present, HHV-7 persists in a latent state in the majority of cases; (iii) the presence of EBV seems to be associated with the diseased state of the patient and site.

Cited by 53 publications

References 42 publications

Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis

Prevalence of Herpesvirus and Correlation with Clinical Parameters in Indian Subjects with Chronic Periodontitis

Herpesviral and “Red Complex” Bacterial Analysis of Acute Apical Abscesses

Herpes Simplex 1 and Periopathogen Role in Peri-implantitis

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