Human fetal osteoblast cell response to self-assembled nanostructures on YSZ-(110) single crystal substrates

Aminuddin, Nur Izzati; Ahmad, Roslina; Ansari, Haris Masood; Zain, Norita Mohd; Akbar, Sheikh A.; Murphy, Belinda

doi:10.1016/j.matdes.2015.12.173

Cited by 8 publications

(4 citation statements)

References 49 publications

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“…39 In addition, on the 4 th day, the cell number on the 3D rGO/PPY/CPP20 scaffold is also higher than that on the 3D rGO/ PPY/CPP10 and 3D rGO/PPY scaffold. These ndings are consistent with the MTT results, thereby further conrming the non-cytotoxicity of the 3D rGO/PPY based scaffolds and the Fig .…”

Section: Ao/eb Double Staining and Uorescence Microscopymentioning

confidence: 95%

“…It has been established that acridine orange (AO) is only able to pass through the intact cell membrane of live cells and embed into the nucleus DNA, which gives bright yellow-green uorescence, while the ethidium bromide (EB) can only penetrate into the membranes of damaged cells and also embed into the nuclear DNA, producing orange-red uorescence. 39,40 Fig. 10 shows the uorescence images of MC3T3-E1 cells cultured at 2 and 4 days on 3D rGO/ PPY, 3D rGO/PPY/CPP10, and 3D rGO/PPY/CPP20 scaffolds, respectively.…”

Section: Ao/eb Double Staining and Uorescence Microscopymentioning

confidence: 99%

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Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds

Jie

Song

et al. 2017

RSC Adv.

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In bone tissue engineering, the 3D macro-scaffold plays an indispensable role as a matrix for cell proliferation, differentiation and tissue formation, for which a bioactive surface is required. Previously, we successfully prepared the 3D macro-reduced graphene oxide/polypyrrole (3D rGO/PPY) scaffold; in the present study, by using casein phosphopeptide (CPP) as a bioactive molecule, we have developed a 3D rGO/PPY/CPP composite scaffold through a simple, but low-cost electrostatic self-assembly method and have explored the application of the composite scaffold in bone tissue engineering. The results have indicated that the CPP successfully modified the backbone of the scaffold, and demonstrated that the developed 3D rGO/PPY/CPP scaffold has excellent hydrophilic behavior and water uptake performance, much better than that of 3D rGO/PPY. In the biomimetic mineralization experiment, the CPP-modified matrix, particularly 3D rGO/PPY/CPP20, could promote the rapid formation of hydroxyapatite in simulated body fluid solution on just the 1 st soaking day. On co-culturing with the MC3T3-E1 cells, the 3D rGO/PPY/CPP20 kept the cells at a higher proliferation state of 5.07 times that of the control group, superior to that of the 3D rGO/PPY/CPP10 (3.88 times) and the 3D rGO/PPY group (2.07 times). The excellent osteoblastic performance of the 3D rGO/PPY/CPP20 composite scaffold can be attributed to the good biological properties of CPP, and the unique 3D macro-structure with a high specific surface area. Our findings suggest that the 3D rGO/PPY/CPP20 can be considered as an attractive scaffold for bone healing and regeneration in the future.

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Section: Ao/eb Double Staining and Uorescence Microscopymentioning

confidence: 95%

Section: Ao/eb Double Staining and Uorescence Microscopymentioning

confidence: 99%

Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds

Jie

Song

et al. 2017

RSC Adv.

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“…[80][81][82][83][84] Ce is known to regulate the proliferation and differentiation of osteoblasts as well as promote hydroxyapatite (HA) precipitation. [85][86][87][88] Like Ti, Ce is a catalyst that can inhibit bacterial proliferation; Ce also exhibits anti-inflammatory capabilities. [37,89,90] Ga has been reported to show antibacterial activity as well as osteoblast proliferation and osteoclast minimization 2016).…”

Section: Biological Analysesmentioning

confidence: 99%

Production of Antibacterial Activity and Bone Cell Proliferation by Surface Engineering of Ga‐ or Mn‐Doped Ceria‐Coated Biomedical Titanium Alloy

et al. 2022

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The present work reports a detailed interpretation of the role of Ga and Mn dopants, solid solubility mechanisms, charge compensation mechanisms, intervalence charge transfer, antibacterial performance, and cell attachment and proliferation. Sol–gel undoped and doped (1, 5, and 9 mol%) CeO2 films are spin‐coated on 3D printed Ti6Al4V biomedical alloy substrates and annealed at 650 °C for 2 h in air. Material characterization includes scanning electron microscopy (SEM), 3D scanning laser confocal microscopy, glancing angle X‐ray diffraction (GAXRD), and X‐ray photoelectron spectroscopy (XPS). In vitro testing includes inhibition of bacterial growth, simulated body fluid (SBF) testing, and cell attachment and proliferation studies. The most significant outcome is that the bioactivity of ceria derives directly from the Ce3+ concentration, which itself results from solid solubility (substitutional and interstitial) and charge compensation and redox. This challenges the common assumption of the dominance of oxygen vacancies in the performance of ceria. The antibacterial activity is dependent on the type, amount, and valence of the dopant, where opposite trends are observed for gram‐positive Staphylococcus aureus and gram‐negative Escherichia coli bacteria. All of the doped samples result in enhanced cell proliferation, although this is greatest at the lowest dopant concentration. Surface hydroxyapatite formation on the samples is achieved by soaking in SBF at 2 weeks and 1 month.

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“…For example, corneal epithelial membrane has features similar to the groove/ridge topography of the nanobar pattern, with dimensions ranging from 20–200 nm . While YSZ ceramic is not a substrate of choice in cell culture studies, proof of concept studies have shown that cells do respond to topography on YSZ surfaces directly. , It has also been demonstrated that self-assembled discrete nanoislands on YSZ-(100) surfaces can be used as master patterns for nanoimprinting important biomedical polymers such as polydimethylsiloxane (PDMS), polystyrene (PS) and ethylene glycol dimethacrylate (EGDMA) . Unlike a pattern of discrete islands on a surface, a ripple pattern is equivalent to its own negative, and therefore it can be transferred in a single step.…”

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confidence: 99%

Spontaneous Rippling and Subsequent Polymer Molding on Yttria-Stabilized Zirconia (110) Surfaces

et al. 2017

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Spontaneous nanoripple formation on (110) surfaces of yttria-stabilized zirconia, YSZ-(110), is achieved by diffusional surface doping with rare-earth oxides. Periodic arrays of parallel nanobars separated by channels (period ∼100 nm) grow out of the dopant sources, covering relatively wide areas of the surface (∼10 μm). The nanobars mound up on the surface by diffusion, exhibiting morphological uniformity and alignment, with their long axis lying parallel to the [11̅0] direction in the YSZ-(110) surface. The process for forming these nanobar arrays can be as simple as sprinkling of rare-earth oxide powder (dopant source) on YSZ-(110) surface and annealing in a high temperature air furnace. However, higher control on dopant dispersion on the surface is demonstrated with other techniques, including photolithography and inkjet printing. The ripple arrays extend anisotropically on the (110) surface, obeying the parabolic growth law, and showing principal values of the rate constant along [11̅0] (maximum) and [001] (minimum), as expected from the symmetry of the (110) surface. The self-patterned ceramic substrates are well-suited for pattern transfer by replica molding, as illustrated by single-step molding with polydimethylsiloxane (PDMS), which is a widely used biomaterial in cell-culture studies.

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Human fetal osteoblast cell response to self-assembled nanostructures on YSZ-(110) single crystal substrates

Cited by 8 publications

References 49 publications

Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds

Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds

Production of Antibacterial Activity and Bone Cell Proliferation by Surface Engineering of Ga‐ or Mn‐Doped Ceria‐Coated Biomedical Titanium Alloy

Spontaneous Rippling and Subsequent Polymer Molding on Yttria-Stabilized Zirconia (110) Surfaces

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