Human Exosomes Accelerate Cutaneous Wound Healing by Promoting Collagen Synthesis in a Diabetic Mouse Model

Zhao, Bo; Zhang, Xingliao; Zhang, Yuanlin; Lu, Yijun; Zhang, Wanting; Lu, Shoutao; Fu, Yu; Zhou, Yang; Zhang, Jun; Zhang, Jing

doi:10.1089/scd.2021.0100

Cited by 41 publications

(26 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent meta-analysis found lower amputation rates and increased wound healing in autologous stem cell treatment randomized controlled trials [ 241 ]. Cell-free therapies using adipose MSC conditioned media [ 182 ] and exosomes [ 207 , 208 , 209 ] to treat wounds are also in development. Some current clinical trials in progress utilize MSCs derived from the umbilical cord (NCT04104451), human placenta (NCT04464213), and adipose tissue (NCT03865394, NCT03916211).…”

Section: Treatment Strategiesmentioning

confidence: 99%

Diabetic Wound-Healing Science

et al. 2021

View full text Add to dashboard Cite

Diabetes mellitus is an increasingly prevalent chronic metabolic disease characterized by prolonged hyperglycemia that leads to long-term health consequences. It is estimated that impaired healing of diabetic wounds affects approximately 25% of all patients with diabetes mellitus, often resulting in lower limb amputation, with subsequent high economic and psychosocial costs. The hyperglycemic environment promotes the formation of biofilms and makes diabetic wounds difficult to treat. In this review, we present updates regarding recent advances in our understanding of the pathophysiology of diabetic wounds focusing on impaired angiogenesis, neuropathy, sub-optimal chronic inflammatory response, barrier disruption, and subsequent polymicrobial infection, followed by current and future treatment strategies designed to tackle the various pathologies associated with diabetic wounds. Given the alarming increase in the prevalence of diabetes, and subsequently diabetic wounds, it is imperative that future treatment strategies target multiple causes of impaired healing in diabetic wounds.

show abstract

Section: Treatment Strategiesmentioning

confidence: 99%

Diabetic Wound-Healing Science

et al. 2021

View full text Add to dashboard Cite

show abstract

“…ASCs EVs delivered functional molecules for non-diabetic/diabetic wound healing via enhancing skin collagen production/angiogenesis/cell proliferation/migration/expression of wound healing-related growth factors, inhibiting apoptosis, promoting skin barrier function repair, reducing inflammation and scar formation, as well as regulating extracellular matrix remodeling [ 12 , 14 , 15 , 32 , 40 , 51 , 53 , 73 , 87 , 88 , 108 , 119 , 138 – 149 , 166 , 175 ]. The underlying mechanisms of action were shown as follows.…”

Section: Resultsmentioning

confidence: 99%

Adipose stem cells-released extracellular vesicles as a next-generation cargo delivery vehicles: a survey of minimal information implementation, mass production and functional modification

et al. 2022

View full text Add to dashboard Cite

Objectives To investigate current situation of minimal information implementation highlighted by minimal information for studies of extracellular vesicles 2018 (MISEV2018) guidelines, and explore technological advances towards mass production and functional modification in aesthetic, plastic and reconstructive surgery. Methods Original articles on extracellular vesicles (EVs) of adipose stem cells (ASCs) were identified. Statistics upon minimal information for EVs research, such as species, cell types, culture conditions, conditioned media harvesting parameters, EVs isolation/storage/identification/quantification, functional uptake and working concentration, were analyzed. Results The items of cell culture conditions such as passage number, seeding density, conditioned media harvesting time, functional uptake and working concentration were poorly documented, with a reporting percentage of 47.13%, 54.02%, 29.89%, 62.07% and 36.21%, respectively. However, there were some studies not reporting information of ASCs origin, culture medium, serum, EVs isolation methods, quantification and identification of EVs, accounting for 3.45%, 10.34%, 6.90%, 3.45%, 18.39% and 4.02%, respectively. Serum deprivation and trophic factors stimuli were attempted for EVs mass production. Several technological advances towards functional modification included hypoxia pre-condition, engineering EVs and controlled release. Presently, ASCs EVs have been applied in multiple fields, including diabetic/non-diabetic wound healing, angiogenesis, inflammation modulation, fat grafting, hair regeneration, antiaging, and healing and regeneration of cartilage/bone/peripheral nerve/tendon. Conclusion Our results highlight normative reporting of ASCs EVs in functional studies to increase reliability and reproducibility of scientific publications. The advances towards mass production and functional modification of ASCs EVs are also recommended to enhance therapeutic effects.

show abstract

“…The disorder of re-epithelialization is closely related to the delayed healing and the occurrence of chronic refractory wounds ( Caedo-Dorantes and Caedo-Ayala, 2019 ). At the same time, a large amount of collagen will be generated during the remodeling period of wound healing with regularly and orderly arrangement, reducing the possibility of scar repair and improving the quality of tissue remodeling ( Liu et al, 2010 ; Zhao et al, 2021 ). Our results showed that compared with the control group, RHC and rFN could significantly accelerate the speed of wound healing, especially in the combined treatment group.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Acute Wounds With Recombinant Human-Like Collagen and Recombinant Human-Like Fibronectin in C57BL/6 Mice Individually or in Combination

Dong

Zhu

Lei

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Wound repair is accomplished by the interaction between the cells involved in the repair and the extracellular matrix (ECM). Collagen is the main component of ECM, which is involved in transduction of signal, transportation of growth factors and cytokines. Fibronectin (FN) is also an important ECM, which participates in the initiation of fibroblast cell (FC) and promotes adhesion, migration, proliferation and differentiation of target cells. Compared with natural protein, the recombinant protein prepared by artificial method has the advantages of poor immunogenicity, wide range of sources, low cost and high activity. In this study, we used recombinant human-like collagen (RHC) and recombinant human-like fibronectin (rhFN) to treat acute wounds in C57BL/6 mice individually or in combination, and explored their effects on wound healing. Our study confirmed that these two recombinant proteins could effectively promote the proliferation, migration and adhesion of FCs. Meanwhile, it could positively regulate the healing speed and quality of acute wounds, re-epithelialization, collagen deposition, inflammation and angiogenesis. Moreover, we proved that the combination of the two was better than the treatment alone. Consequently, it has a good prospect as a new tissue material in the field of skin repair.

show abstract

Human Exosomes Accelerate Cutaneous Wound Healing by Promoting Collagen Synthesis in a Diabetic Mouse Model

Cited by 41 publications

References 50 publications

Diabetic Wound-Healing Science

Diabetic Wound-Healing Science

Adipose stem cells-released extracellular vesicles as a next-generation cargo delivery vehicles: a survey of minimal information implementation, mass production and functional modification

Treatment of Acute Wounds With Recombinant Human-Like Collagen and Recombinant Human-Like Fibronectin in C57BL/6 Mice Individually or in Combination

Contact Info

Product

Resources

About